Factors driving the distribution and role of AOA and AOB in <i>Phragmites communis</i> rhizosphere in riparian zone

Wang, Xiaomin; Wang, Shanyun; Shi, Guoshuai; Wang, Weidong; Zhu, Guibing

doi:10.1002/jobm.201800581

Cited by 14 publications

(6 citation statements)

References 63 publications

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“…In this study, the NH 4 + ‐N concentration of the sediment has a negative correlation with comammox amoA abundance, which was similar to the findings of a study of tidal flat sediments in the Yangtze River estuary (Jiang et al ). Moreover, the NH 4 + ‐N concentration of the sediment has no significant correlation with AOA amoA or AOB amoA gene, which was similar to the study of rhizosphere sediments of riparian zone (Wang et al ). This was likely due to the relatively high NH 4 + ‐N concentration in lake sediments (19·12–44·13 mg kg −1 ) in this study, compared to that in soils (7–20 mg kg −1 ) (Shi et al ).…”

Section: Discussionsupporting

confidence: 83%

Diversity and abundance of comammox bacteria in the sediments of an urban lake

Xu¹,

Lü

Liu³

et al. 2020

J Appl Microbiol

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Aims Although comammox have been discovered in a variety of ecosystems, there are few studies in urban lakes. This paper attempted to confirm whether this ammonia‐oxidizing microbe exists in urban lakes and to determine the factors influencing its existence. Methods and Results This study investigated the diversity and abundance of comammox bacteria in sediments of a typical urban lake in China, and their ecological relationship with other ammonia‐oxidizing micro‐organisms. The phylogenetic analysis indicated that comammox clade A existed in the sediment of Lake Donghu, and the comammox bacteria co‐existed with ammonia‐oxidizing archaea (AOA), ammonia‐oxidizing bacteria (AOB) and anaerobic ammonia‐oxidizing (anammox) bacteria in the sediment of this lake. The abundances of the ammonia monooxygenase subunit A (amoA) genes for comammox, AOA, AOB and anammox 16S rRNA were 2·43 × 108, 1·07 × 108, 3·24 × 107 and 3·21 × 1011 copies per gram dry sediment respectively. Moreover, the amoA gene abundance of comammox was positively correlated with that of AOA and AOB. The redundancy analysis showed that the abundance of the comammox amoA gene was negatively correlated with the concentration of main indicators for nitrogen status in both the sediment and the water column, indicating that eutrophication may inhibit the growth of comammox bacteria. Conclusions Comammox bacteria play an important ecological role in the nitrogen cycle of urban lake sediments. Significance and Impact of the Study Our results indicated comammox bacteria were widespread in urban lakes and eutrophication may inhibit their growth.

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Section: Discussionsupporting

confidence: 83%

Diversity and abundance of comammox bacteria in the sediments of an urban lake

Xu¹,

Lü

Liu³

et al. 2020

J Appl Microbiol

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“…There were also significant and negative correlations between AOA abundance and sediment metals (Table 1), suggesting that higher metal levels in vegetated sediments maybe toxic to AOA (Liu et al 2014). In line with our results, significantly higher AOA and AOB abundances in the bulk soils than in the Phragmites communis rhizosphere soils were also found in the riparian zone (Wang et al 2019).…”

Section: Abundances Of Aob and Aoa In The Seagrass Systemsupporting

confidence: 89%

“…It was interesting that in the N-limited (NH 4 + ≤ 660 μmol g −1 ) Z. marina sands of Tanoura Bay, Nitrosospira dominated in the in situ AOB community, but after the enrichment incubation with 4 mM NH 4 + , the dominant AOB population shift to Nitrosomonas (Ando et al 2009). Furthermore, from the view of habitat selection, Nitrosomonas mainly occurs in marine sediments (Zhang et al 2015b;Ling et al 2018), while Nitrosospira was more adaptable in soils and freshwater sediments (Jia and Conrad 2009;Wang et al 2019) (Table 3).…”

Section: Seagrass Colonization Selected Specific Lineages Of Ammonia Oxidizersmentioning

confidence: 99%

Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments

et al. 2021

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Purpose Seagrasses accelerate sedimentation, release oxygen and organic matter through their roots, and compete with ammonia oxidizers for ammonia/ammonium in surface sediments and overlying water, all of which can influence benthic aerobic nitrification. To understand the effects of seagrass vegetation on benthic nitrification, the heterogeneity of nitrification activities and functional microbial communities between seagrass-vegetated and adjacent bare sediments was investigated. Materials and methods Surface (0-5 cm) sediments were sampled from a Zostera marina-colonized coastal lagoon in northern China. The potential nitrification rates (PNR) and relative contributions of ammonia-oxidizing bacteria (PNR aob ) and ammoniaoxidizing archaea (PNR aoa ) were determined based on the sediment slurry incubation with kanamycin inhibition method. The abundances and community compositions of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were determined using qPCR, clone library, and high-throughput sequencing. Results and discussionThe total PNR (PNR total ) in the seagrass-colonized sediments were significant higher than those in the bare sediments (P < 0.05), to which PNR aob contributed 59.7% and 55.8% in vegetated and unvegetated sediments, respectively. The concentrations of chlorophyll a and dissolved inorganic nitrogen were key factors determining benthic PNR. AOB outnumbered AOA by one order of magnitude by targeting amoA gene abundance, and no significant difference (P > 0.05) in amoA gene abundance and diversity was observed between the two habitats. Most AOB genotypes were affiliated to Nitrosomonas, of which NL7 was selectively enriched in the vegetated region, while N. cryotolerans prevailed in the bare. Two distinct AOA groups Crenarchaeota 1.1b and Crenarchaeota 1.1a dominated in vegetated and unvegetated sediments, respectively. Dissolved oxygen of overlying water and TOC:TN of sediment significantly influenced AOB community, while AOA community was strongly driven by nitrate and metal iron in sediments. Conclusions Seagrass vegetation substantially enhances nitrification potential and selects specific ammonia oxidizers in coastal sediments.

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“…The dominant genera of AOB, Nitrosospira , accounted for about 99% of all AOB sequences, which may be due to the low NH 4 + level in the riparian zone (Table 1), where Nitrosospira has a stronger competitive survival ability than Nitrosomonas . These results showed that Nitrosospira may be essential for the ammonia oxidation of bacteria in the riparian zone [48].…”

Section: Discussionmentioning

confidence: 99%

Distribution of ammonia oxidizers and their role in N₂O emissions in the reservoir riparian zone

Wang

Huang

et al. 2022

J Basic Microbiol

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As a transitional boundary between terrestrial and aquatic ecosystems, the riparian zone is considered a hotspot for N 2 O production because of the active nitrogen processes. Ammoxidation is an important microbial pathway for N 2 O production, but the distribution of ammonia oxidizers under different land-use types in the reservoir riparian zone and what role they played in N 2 O emissions are still not clear. We investigated spatiotemporal distributions of ammonia-oxidizing archaea (AOA) and bacteria (AOB) and their role in N 2 O emissions in different land-use types along the riparian zone of Miyun Reservoir: grassland, sparse woods, and woodland. We found significant differences in both AOA abundance and AOB diversity indices among land-use types. AOA and AOB communities were significantly separated by different land-use types. The main drivers to determine the distribution of ammonia-oxidizing microbial community were soil water content, NH 4 + , NO 3 − , and total organic carbon (TOC). In situ N 2 O flux was highest in woodland with a mean value of 12.28 μg/m 2 •h, and it was substantially decreased by 121% and 123% in sparse woods and grassland. TOC content was decreased by 20% and 40% in sparse woods and grassland compared with woodland, and it was significantly positively correlated with in situ N 2 O flux. Meanwhile, AOB diversity indices were significantly correlated with in situ N 2 O flux. These results showed that the heterogeneity of physicochemical properties among different land-use types affected the community of AOA and AOB in riparian zones. AOB not AOA, and community diversity rather than abundance, played a role in N 2 O emissions.

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Factors driving the distribution and role of AOA and AOB in Phragmites communis rhizosphere in riparian zone

Cited by 14 publications

References 63 publications

Diversity and abundance of comammox bacteria in the sediments of an urban lake

Diversity and abundance of comammox bacteria in the sediments of an urban lake

Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments

Distribution of ammonia oxidizers and their role in N₂O emissions in the reservoir riparian zone

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Factors driving the distribution and role of AOA and AOB in Phragmites communis rhizosphere in riparian zone

Cited by 14 publications

References 63 publications

Diversity and abundance of comammox bacteria in the sediments of an urban lake

Diversity and abundance of comammox bacteria in the sediments of an urban lake

Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments

Distribution of ammonia oxidizers and their role in N2O emissions in the reservoir riparian zone

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Distribution of ammonia oxidizers and their role in N₂O emissions in the reservoir riparian zone