Factors effecting chitinase activity of Rhizobium sp. from Sesbania sesban

Abstract

Twenty six Rhizobium strains isolated from root nodules of Sesbania sesban were studied for chitinase activity on chitin agar plates. Among them, only 12 strains showed chitinase activity. The strain showing the highest chitinase activity was selected based on maximum clear zone/colony size ratio on chitin agar plates and chitinase activity in culture filtrate. The strain was identified as Rhizobium sp. which showed a high degree of similarity with Rhizobium radiobacter (=Agrobacterium radiobacter). The cultural and nutritional conditions were optimized for maximum chitinase activity. The Rhizobium sp. exhibited maximum chitinase activity after 36 h of incubation, at neutral pH. Among the different nutritional sources, arabinose and yeast extract were found to be good inducers for chitinase activity. Rhizobium sp. could degrade and utilize dead mycelia of Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum and Fusarium udum.

Characterization of Lysinibacillus fusiformis strain S4C11: In vitro, in planta, and in silico analyses reveal a plant-beneficial microbe

“…The following biochemical in vitro assays were carried out to determine the presence of some common plant-growth promotion or plant-associated traits: (i) siderophore production, using CAS agar medium as described by Alexander and Zuberer (1991); (ii) catalase activity, tested by dripping 3% hydrogen peroxide directly on the bacterial colonies; (iii) production of indole acetic acid, using the colorimetric method described by Pilet and Chollet (1970); (iv) chitinase production, using the medium reported by Sridevi et al (2008).…”

Chitinolytic Microorganisms and Their Possible Application in Environmental Protection

“…NRG4 Fusarium moniliforme , Aspergillus niger , Mucor rouxi , Rhizopus nigricans [17] Alcaligenes xylosoxydans Fusarium sp. Rhizoctonia bataticola [113] Stenotrohomonas maltophila Fusarium solani , F. oxysporum , R. solani , A. alternata [46, 100] Rhizobium sp Aspergillus flavus , Aspergillus niger , Curvularia lunata , F. oxysporum , Fusarium udum [97] Serratia marcescens strain B2 Botrytis cinerea [95] Vibrio pacini Mucor racemosus , Trichoderma viride , Zygorhynchus heterognmus , Candida albicans [3] Streptomyces halstedii AJ-7 Alternaria alternata , B. cinerea , S. lycopersici , F. oxysporum [49] Streptomyces hygroscopicus Colletotrichum gloeosporioides , Sclerotium rolfsii [80] Streptomyces tendae TK-VL_333 Aspergillus niger , F. oxysporum [51] Streptomyces sp. DA11 Aspergillus niger , Candida albicans [37] Streptomyces roseolus DH Aspergillus spp., Rhizopus chinensis , Penicillium spp., Mucor spp.[47] Streptomyces sporovirgulis Alternaria alternata[104] Streptomyces rimosus Alternaria alternata , F. solani [105]…”

Chitinases As The Key To The Interaction Between Plants And Microorganisms

“…Jeden z tych szczepów sprawdzono pod kątem aktywności przeciwgrzybiczej przeciwko Aspergillus flavus, Aspergillus niger, Curvu laria lunata, Fusarium oxysporum i Fusarium udum. Wyniki sugerują rolę ochronną enzymów podczas symbiozy [88]. W brodawkach korzeniowych innych roślin w tym Vigna trilobata stwierdzono występowanie endofitycznych, niesymbiotycznych bakterii jak Bacil lus altitudinis, Paenibacillus sp., Ensifer xinjiangense i Agrobacterium tumefaciens, u których potwierdzono produkcję chitynazy [47].…”