Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant

Zhang, Chao; Fu, Shaoping; Tang, Gaijuan; Hu, Xinwen; Guo, Jiao

doi:10.1007/s11627-013-9530-z

Cited by 22 publications

(9 citation statements)

References 47 publications

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“…A concentration of 1.0 mg/L TDZ gave the highest number of shoot buds (p < 0.01), compared to other concentrations of TDZ. These results are in agreement with the study by Zhang et al [15], where the same concentration of 1.0 mg/L of TDZ is also reported to give highest shoot bud formation ( Table 2). All the primers (Table 3) applied in this study were selected according to Grativol et al [28] and Kumar et al [29].…”

Section: Resultssupporting

confidence: 93%

“…For example, a high concentration of cytokinin (6 mg/L BAP) applied to the initial culture can lead to somaclonal variation in J. curcas using axillary buds as explant material [6]. Some studies have reported that the application of TDZ at high concentrations leads to phenotypic abnormalities and to the inhibition of shoot regeneration and root development in J. curcas [15], as well as in other plants [36]. This is probably related to the somaclonal variation (genetic instability) that was observed following exposure to a high TDZ concentration of 4.0 mg/L, at which genetic variation occurred in this study (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

“…The in vitro propagation of J. curcas using thidiazuron (TDZ) as the basic plant growth regulator has been reported by some researchers [14][15][16]. TDZ is a substituted phenylurea compound that has been shown to possess both auxin-and cytokinin-like effects, although it is structurally different from either auxins or purinebased cytokinins [17].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Nasir

Anuar

Zainuddin

et al. 2016

Biotechnology & Biotechnological Equipment

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Genetic homogeneity is known to be the most important prerequisite in the micropropagation of Jatropha curcas L. to produce true-to-type plants. The detection of genetic homogeneity in clonal micropropagation for elite plants at an early stage is required, to avoid any increase in variation in the next stage of micropropagation. The genetic homogeneity was assessed during shoot bud formation from petiole explants of J.curcas (P1 £ P3) hybrid with different concentrations of thidiazuron (TDZ) in a range of 0.5-4.0 mg/L using inter simple sequence repeat (ISSR) markers. Out of 23 ISSR primers, 16 primers produced clear, distinct and reproducible bands. A total of 96 bands, ranging in size from 100 to 1013 bp were generated. Based on the band data, a total of 94 bands were monomorphic (98%) and two bands were polymorphic (2%). All banding patterns from the shoot buds induced by 0.5, 1.0 and 2.0 mg/L TDZ were monomorphic, but 4.0 mg/L gave 2% polymorphism. These findings indicated that concentrations of 0.5, 1.0 and 2.0 mg/L TDZ did not trigger any somaclonal variation and could, therefore, be considered suitable for application in clonal micropropagation of J. curcas hybrid using petioles as explant material.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Nasir

Anuar

Zainuddin

et al. 2016

Biotechnology & Biotechnological Equipment

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“…In this season collection of explants were achieved an average of 39.8, 28.9 and 10.9 shoots per explants for the genotypes G1, G2 and G3, respectively (Figure 2). These averages were higher than those reported by other authors (3.5 to 10 shoots per explants) (Shukla et al, 2013;Zhang et al, 2013). For all genotypes, the control treatments without growth regulators (treatment 1) did not regenerated none adventitious shoot.…”

Section: Foliar Explants Organogenesiscontrasting

confidence: 60%

Micropropagation of Jatropha curcas superior genotypes and evaluation of clonal fidelity by target region amplification polymorphism (TRAP) molecular marker and flow cytometry

Franco¹,

Marques²,

Siqueira³

et al. 2014

Afr. J. Biotechnol.

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“…There are other reports of adventitious shoot morphogenesis producing seven, six and eleven shoots from petiole, stem and leaf explants, respectively (Verma and Verma, 2015). In other studies, direct organogenesis of petiole resulted in seven adventitious shoots (Liu et al, 2015), and mature leaves produced up to 2.98 shoots per explant (Zhang et al, 2013).…”

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confidence: 77%

Optimization of in Vitro Adventitious Shoot Induction in Jatropha Curcas by Response Surface Methodology.

Jose¹

2019

IJAR

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Jatropha curcas is a second generation biofuel crop that is also an important biological resource. Meeting increasing biofuel demand requires wider cultivation of specialized varieties, highlighting the need for massive culture techniques such as in vitro micropropagation. Key stages in plant tissue culture processes, such as adventitious shoot induction, can be optimized to increase micropropagation protocol efficiency. Response surface methodology, a statistical tool for this purpose, was applied to optimize in vitro induction of adventitious shoots in the J. curcas micropropagation process. A preliminary assay was done in which 6-(γ, γ-dimethylallylamino) purine and indolacetic acid were selected as the factors to optimize. A 2 2 central composite design with four central points was then applied to fit it to a second grade model and a rotatable design applied to produce the final model and surface graphics. Focused on maximum point, the surface model design identified maximum adventitious shoot induction to be 21 shoots per explant in Murashige and Skoog medium at 75% ionic strength and containing 83.3 µM 6-(γ, γ-dimethylallylamino) purine and 1.3 µM indolacetic acid. This is the first report in which response surface methodology was found to be an effective statistical technique for optimizing adventitious shoot induction in Jatropha curcas.

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Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant

Cited by 22 publications

References 47 publications

Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Evaluation of genetic homogeneity of Jatropha curcas L. hybrid at an early stage of shoot bud formation from petioles using ISSR marker

Micropropagation of Jatropha curcas superior genotypes and evaluation of clonal fidelity by target region amplification polymorphism (TRAP) molecular marker and flow cytometry

Optimization of in Vitro Adventitious Shoot Induction in Jatropha Curcas by Response Surface Methodology.

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