Factors influencing osteoblast maturation on microgrooved titanium substrata

Surface functionalization of microgrooved titanium with dual growth factor-releasing nanoparticles for synergistic osteogenic differentiation of human mesenchymal stem cells

“…5). In accordance with the ALP activity assay results from our previous study [27], we show here that sustained release of BMP-2 by catechol-functionalized Ti adhesive polymer nanoparticles enhanced osteogenic activity of MSCs. However, MSCs cultured on E60/10IGF1 consistently exceeded those grown on E60/10BMP2 in ALP activity and osteoblastic differentiation at all time points in the culture.…”

“…Cells were then washed with Dulbecco's Modified Eagle Medium (DMEM; WelGene, Daegu, Korea) and cultured in osteogenic medium [DMEM (WelGene) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 50 g/ml ␣-ascorbic acid (Sigma-Aldrich, St. Louis, MO, USA), 10 mM ␤-glycerophosphate (Sigma-Aldrich), and 100 mM dexamethasone (Sigma-Aldrich)] at 37 • C under 5% CO 2 . After 10 and 20 days of culture in the osteogenic medium, we performed the ALP activity assay as previously described [27]. The reaction products were transferred to 96-well plates and monitored using a microplate reader (Bio-Rad) at 405 nm.…”

Cell architecture–cell function dependencies on titanium arrays with regular geometry

“…In vitro studies on cellular behavior of osteoblast-like cells on titanium surfaces with different roughness profiles have been shown to affect different cell reactions like cell attachment, proliferation and differentiation (e.g. alkaline phosphatase) in a roughness-dependent matter [24,25,28,31,32]. Luethen et al [24] and Anselme et al [31] were able to establish correlations between the roughness of stochastically structured titanium surfaces and the cell function of MG-63 osteoblasts, such as cell growth and integrin expression.…”

An accelerated buoyancy adhesion assay combined with 3-D morphometric analysis for assessing osteoblast adhesion on microgrooved substrata

“…Nevertheless, various cell types, such as mesenchymal stem cells (Gerecht et al, 2007), osteoblasts (Kenar et al, 2006;Erdoğan et al, 2011), fibroblasts (Wang et al, 2010) and epithelial cells (den Braber et al, 1996;Khang et al, 2008) have shown to increase their proliferation rates on micro-grooved surfaces. Similarly, regarding osteogenic differentiation, some studies reported increased osteogenic differentiation markers on grooved surfaces (Mata et al, 2009;Lee et al, 2010), while others suggest no significant effects or reduction of cell differentiation (Bruinink and Wintermantel, 2001;Kenar et al, 2006;Holthaus et al, 2012). While the wide range of materials, cell types and experimental conditions again makes it difficult to draw clear universal conclusions, these studies do suggest that the presence of grooves can affect cell morphology, gene expression, proliferation and the strength of adhesion.…”