Factors influencing survival and growth of mammalian cells exposed to hypothermia. I. Effects of temperature and membrane lipid perturbers

Kruuv, J.; Glofcheski, D.J.; Cheng, Kwan Hon; Campbell, Shelagh D.; Al-Qysi, Hisham; Nolan, William; Lepock, James R.

doi:10.1002/jcp.1041150212

Cited by 47 publications

(19 citation statements)

References 32 publications

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“…The slope of the Arrhenius plot changes to an apparent 'negative activation energy' in the temperature range of 8-08C, which is indicative of a transition near 88C. A break in the Arrhenius plot for killing has been seen in several mammalian cell lines between 5-108C 8 . The Arrhenius curve in the hyperthermic region illustrates the normal properties of hyperthermic killing.…”

Section: Arrhenius Plots Of Growth and Killingmentioning

confidence: 99%

“…Evidence regarding the nature of thermal damage and the temperature range over which it occurs can be deduced from Arrhenius plots of killing and growth over the hyperthermic, normothermic, and hypothermic temperature ranges 8 . As shown in figure 2, growth increases uniformly from 30-408C for V79 cells with an activation energy of 18 kcal/mole, implying that the rate of growth and division is limited by metabolic processes.…”

Section: Arrhenius Plots Of Growth and Killingmentioning

confidence: 99%

“…Hypothermic killing below 88C and hyperthermic killing above 418C are induced by transitions. The critical temperature at 88C is thought to be due to a lipid transition 8 , but nearly all the evidence indicates that the transition causing hyperthermic killing is due to protein denaturation 1 . The Arrhenius analysis is based on killing being controlled by a molecular process with the same activation energy.…”

Section: Arrhenius Plots Of Growth and Killingmentioning

confidence: 99%

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Cellular effects of hyperthermia: relevance to the minimum dose for thermal damage

Lepock

2003

International Journal of Hyperthermia

323

224

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The specific mechanism of cell killing by hyperthermia is unknown, but the high activation energy of cell killing and other responses to hyperthermia suggest that protein denaturation is the rate-limiting step. Protein denaturation can be directly monitored by differential scanning calorimetry and in general there is a good correlation between protein denaturation and cellular response. Approximately 5% denaturation is necessary for detectable killing. Protein denaturation leads to the aggregation of both denatured and native protein with multiple effects on cellular function.

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Section: Arrhenius Plots Of Growth and Killingmentioning

confidence: 99%

Section: Arrhenius Plots Of Growth and Killingmentioning

confidence: 99%

Section: Arrhenius Plots Of Growth and Killingmentioning

confidence: 99%

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Cellular effects of hyperthermia: relevance to the minimum dose for thermal damage

Lepock

2003

International Journal of Hyperthermia

323

224

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“…However, the involving between hypothermia and hypoxia was studied by many physiologists who studied on cardiac metabolism of hibernating mammals [9,10,11] . It was presumed that eosinophilic cells stained by HE result from hypoxic changes of cardiac cells, since the red or orange color cells were detected around the infarction sit in the cardiac infarction cases in this study.…”

Section: Discussionmentioning

confidence: 99%

Histopathological Characteristics of Human Cardiac Tissues in Accidental Hypothermia Using Conventional Staining Techniques

Wingenfeld¹,

Sakaguchi²,

Nakagawa³

2013

LSMR

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Abstract-We examined characteristics or cardiac cell damage in the myocardium obtained from individuals who died due to hypothermia or coldwater immersion. We could find four kinds of histological changes in the heart tissues obtained from hypothermia and coldwater immersion death. Although the red or orange colored cardiac cells, vacuolar cardiac cells and contraction band of the cardiac cells were observed in the heart from individuals with cardiac infarction, the frequency and intensity of the finding was remarkable in hypothermic death, and closely adhered cardiac cells were detected only in the myocardium from hypothermic or coldwater immersion death. We additionally discussed the mechanisms of development of these findings by comparing the results obtained in this examination with some reports in the literatures. Accidental hypothermia is defined as unintentional fall in core temperature under 35℃, and sever hypothermia is usually defined as under 28℃. Without through review of circumstances, the diagnosis of environmentally induced hypothermia is difficult at an autopsy, however hypothermia effects the cardiovascular, hematological, neurological, respiratory, renal, metabolic, and gastrointestinal systems [1] . Keywords-HypothermicIn this study we examined histo-pathological phenomena of the cardiac muscle obtained from individuals who died due to accidental hypothermia or immersion in cold fresh water. We found several changes occurred in the cardiac tissues in them, and are able to say that Azan staining may give the easily detected characteristics in the cardiac tissues from individuals who died due to hypothermia and/or immersion in cold water as a routine staining compared with HE staining. II MATERIAL AND METHODSWe performed 16 autopsies of hypothermic death at Department of Legal Medicine, Shiga University of Medical Science and Osaka Medical Examiner's Office during the last 4 years. The myocardium from 13 of 16 cases were examined in this study. Thirteen cases contain two old women who lived alone and died from hypothermia together with head injury after stumbling or fall from a stairs in their houses, and one child who died from abuse. The other 10 victims were found their death inside or around their houses and no remarkable injury was found on their body except the erythema on their knees in some cases. The eliminated three cases in which the victims showed prolonged postmortem interval were not suitable for the histological research. We also examined the myocardium from three cases as the comparative cases in which individuals died from drowning after immersion in a lake or canals in winter, that is, a young man was forced to go into a lake in winter, and in the last two cases young woman and man separately fell into canals with a motorcycle or a bicycle. In the last two cases, climbing traces were remained on the canal banks. Additionally we used the myocardium from traffic accidents cases with or without brain and/or cardiac injury, and cardiac infarction cases with bleeding and leukocyte i...

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“…Hypothermic condition per se induces losses of ionic homeostasis, membrane integrity, and membrane associated transporter and enzyme functions, resulting in metabolic, structural and functional cell injury (92–94). In particular, hypothermia has been shown to induce apoptosis in various types of cultured cells by increasing the opening of the permeability transition pores and subsequent mitochondrial swelling, and generation of ROS and direct DNA damage, as well as by an activation of proteasomes and serine proteases (95–98).…”

Section: Co Delivery During Cold Storage and Inhibition Of Apoptosismentioning

confidence: 99%

Use of carbon monoxide in minimizing ischemia/reperfusion injury in transplantation

Ozaki

Kimura

Murase

2012

Transplantation Reviews

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Although carbon monoxide (CO) is known to be toxic due to its ability to interfere with oxygen delivery at high concentrations, mammalian cells endogenously generate CO primarily via the catalysis of heme by heme oxygenases (HO). Recent findings have indicated that HO and generation of CO serve as a key mechanism to maintain the integrity of the physiological function of organs, and supported the development of a new paradigm that CO, at low concentrations, functions as a signaling molecule in the body and exerts significant cytoprotection. Consequently, exogenously delivered CO has been shown to mediate potent protection in various injury models through its anti-inflammatory, vasodilating, and anti-apoptotic functions. Ischemia/reperfusion (I/R) injury associated with organ transplantation is one of the major deleterious factors limiting the success of transplantation. I/R injury is a complex cascade of interconnected events involving cell damage, apoptosis, vigorous inflammatory responses, microcirculation disturbance, and thrombogenesis. CO has a great potential in minimizing I/R injury. This review will provide an overview of the basic physiology of CO, preclinical studies examining efficacy of CO in I/R injury models, and possible protective mechanisms. CO could be developed to be a valuable therapeutic molecule in minimizing I/R injury in transplantation.

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Factors influencing survival and growth of mammalian cells exposed to hypothermia. I. Effects of temperature and membrane lipid perturbers

Cited by 47 publications

References 32 publications

Cellular effects of hyperthermia: relevance to the minimum dose for thermal damage

Cellular effects of hyperthermia: relevance to the minimum dose for thermal damage

Histopathological Characteristics of Human Cardiac Tissues in Accidental Hypothermia Using Conventional Staining Techniques

Use of carbon monoxide in minimizing ischemia/reperfusion injury in transplantation

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