Factors Influencing the in Vitro Cultivation of Common Bean (Phaseolus Vulgaris L.) Leaf Petioles

Svetleva, D.; Velcheva, Margarita; Dimova, D.; Ivanova, K. P.; Petkova, S.

doi:10.1080/13102818.2001.10819101

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Adventitious shoot regeneration was also noticed in the culture of diploid I. indigotica although the number of shoots per explant was not given (Peng and Wang 1994). The percentage of hypocotyl explants that formed shoots represents the response capacity of the explant to the medium and the number of shoots per explant represents the capacity of the explants to produce shoots, which has been found to be influenced by many factors such as explant type (Pablo et al 2002), medium composition and hormone combination in the medium (Bischoff and Mahn 2000, Svetleva et al 2001, Yokoya and Handro 2002 and culture condition (Chen and Chang 2002). GB5 -Gamborg's B 5 medium.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Efficient Regeneration of Tetraploid Isatis indigotica Plants via Adventitious Organogenesis from Hypocotyl Explants

Zhang¹,

Kai²,

Xu³

et al. 2004

Biologia plant.

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An efficient in vitro plant regeneration system via hypocotyl segments of tetraploid Isatis indigotica Fort. was established. Murashige and Skoog's (MS) and Gamborg's (GB 5 ) media were found to be superior to White medium for promoting shoot regeneration. The highest shoot regeneration (92 %) was achieved from hypocotyls cultured on MS medium containing 8.9 µM benzyladenine (BA) and 2.7 µM naphthaleneacetic acid (NAA), with an average of 4.2 shoots developed per explant. Plant regeneration was also improved when the explants were cultured in MS basal medium containing 3 % (m/v) sucrose and grown under a 12-h photoperiod. The developed shoots were well rooted in a half-strength MS medium supplemented with 0.5 µM indole-3-butyric acid (IBA) and were morphologically normal after transfer to soil.

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Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Efficient Regeneration of Tetraploid Isatis indigotica Plants via Adventitious Organogenesis from Hypocotyl Explants

Zhang¹,

Kai²,

Xu³

et al. 2004

Biologia plant.

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“…It is known that the physiological status of initial plants (used for explant sources), is of importance for successful plant regeneration (15,23).…”

Section: Introductionmentioning

confidence: 99%

Seed Precultivation on Media Supplimented with Different Hormones and Its Influence on Callus Growth in Common Bean (Phaseolus Vulgaris L.)

Svetleva

Dimova

Irikova

et al. 2001

Biotechnology & Biotechnological Equipment

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Factors influencing shoot regeneration from cotyledons of tetraploid Isatis indigotica fort

Zhang

Sun

et al. 2003

In Vitro Cell. Dev. Biol. - Plant

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Factors Influencing the in Vitro Cultivation of Common Bean (Phaseolus Vulgaris L.) Leaf Petioles

Abstract: ABSTRACT

Cited by 3 publications

References 16 publications

Efficient Regeneration of Tetraploid Isatis indigotica Plants via Adventitious Organogenesis from Hypocotyl Explants

Efficient Regeneration of Tetraploid Isatis indigotica Plants via Adventitious Organogenesis from Hypocotyl Explants

Seed Precultivation on Media Supplimented with Different Hormones and Its Influence on Callus Growth in Common Bean (Phaseolus Vulgaris L.)

Factors influencing shoot regeneration from cotyledons of tetraploid Isatis indigotica fort

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