This study was designed to achieve the following three objectives. The first objective was to ascertain the current status of sport and exercise engagement among nurses. The second objective was to examine factors affecting nursesʼ sport and exercise activities, and the third objective was to examine the relationship between nursesʼ sport and exercise activities and their job satisfaction and intention to continue working intention to stay. Design: Cross-sectional design was used. Methods: An anonymous self-administered questionnaire survey was conducted targeting 1,091 nurses at two university hospitals. Results: After excluding male nurses and missing data, the data of 891 female nurses were used in the final analyses. The findings can be summarized into the following three points; the first finding was that more than half of the nurses have not engaged in sport and exercise in the past year and running, jogging, and marathon training were the most popular among those who have engaged, the second finding was that the sport and exercise engagement rate of nurses with pre-primary children tends to be lower, and the third finding was that nurses who engaged in sport and exercise 1-2 times a week tended to have higher levels of job satisfaction and intention to continue working in terms of their relationship with their superiors, overtime and workload, approval, and sense of fulfillment compared to nurses who did not engage in sport and exercise at all. Conclusions: This research suggests the importance for medical institutions such as hospitals to try and promote engagement in sport and exercise among nurses from the viewpoint of job satisfaction and retention of nurses.