Faint electric treatment-induced rapid and efficient delivery of extraneous hydrophilic molecules into the cytoplasm

Hasan, Mahadi; Nishimoto, Akinori; Ohgita, Takashi; Hama, Susumu; Kashida, Hiromu; Asanuma, Hiroyuki; Kogure, Kentaro

doi:10.1016/j.jconrel.2016.02.048

Cited by 24 publications

(24 citation statements)

References 25 publications

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“…The skin has a barrier function that protects the body from invasion of environmental substances and microorganisms; therefore, skin patches use various systems to enhance physical or chemical permeation to disrupt the barrier . Representative physical enhancers are iontophoresis , sonophoresis , electroporation , and microneedles . Chemical enhancers include some fatty acids , terpenes , esters , alcohols , peptides , and nanosized carriers such as lipid vesicles , and solid‐in‐oil (S/O) nanodispersions .…”

Section: Introductionmentioning

confidence: 99%

Solid‐in‐oil nanodispersions for transdermal drug delivery systems

Kitaoka

Wakabayashi

Kamiya

et al. 2016

Biotechnology Journal

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Transdermal administration of drugs has advantages over conventional oral administration or administration using injection equipment. The route of administration reduces the opportunity for drug evacuation before systemic circulation, and enables long‐lasting drug administration at a modest body concentration. In addition, the skin is an attractive route for vaccination, because there are many immune cells in the skin. Recently, solid‐in‐oil nanodisperison (S/O) technique has demonstrated to deliver cosmetic and pharmaceutical bioactives efficiently through the skin. S/O nanodispersions are nanosized drug carriers designed to overcome the skin barrier. This review discusses the rationale for preparation of efficient and stable S/O nanodispersions, as well as application examples in cosmetic and pharmaceutical materials including vaccines. Drug administration using a patch is user‐friendly, and may improve patient compliance. The technique is a potent transcutaneous immunization method without needles.

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Section: Introductionmentioning

confidence: 99%

Solid‐in‐oil nanodispersions for transdermal drug delivery systems

Kitaoka

Wakabayashi

Kamiya

et al. 2016

Biotechnology Journal

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“…[4] Previously, we reported the RNAi effects of typical luciferase-siRNA by fET and lipofectamine 2000(LFN)/luciferase-siRNA lipoplex on luciferase activity of B16-F1 cells. [12] The silencing effect of dSC iRed/LFN lipoplex was almost the same as that of LFN/luciferase-siRNA lipoplex. Although LFN/luciferase-siRNA lipoplex is known as the potent siRNA vector, the silencing effect was not so potent.…”

Section: Resultsmentioning

confidence: 92%

“…[12] The difference between siRNA/ISMB and dSC iRed was attributed to their molecular sizes. Molecular sizes of siRNA/ISMB and dSC iRed were c .14,000, 11,200 and 225,000, respectively.…”

Section: Resultsmentioning

confidence: 99%

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The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment

Hasan

Tarashima

Fujikawa

et al. 2016

Science and Technology of Advanced Materials

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An intelligent shRNA expression device (iRed) contains the minimum essential components needed for shRNA production in cells, and could be a novel tool to regulate target genes. However, general delivery carriers consisting of cationic polymers/lipids could impede function of a newly generated shRNA via electrostatic interaction in the cytoplasm. Recently, we found that faint electric treatment (fET) of cells enhanced delivery of siRNA and functional nucleic acids into the cytoplasm in the absence of delivery carriers. Here, we examined fET of cells stably expressing luciferase in the presence of iRed encoding anti-luciferase shRNA. Transfection of lipofectamine 2000 (LFN)/iRed lipoplexes showed an RNAi effect, but fET-mediated iRed transfection did not, likely because of the endosomal localization of iRed after delivery. However, fET in the presence of lysosomotropic agent chloroquine significantly improved the RNAi effect of iRed/fET to levels that were higher than those for the LFN/iRed lipoplexes. Furthermore, the amount of lipid droplets in adipocytes significantly decreased following fET with iRed against resistin in the presence of chloroquine. Thus, iRed could be a useful tool to regulate target genes following fET-mediated cytoplasmic delivery with endosomal escape devices.

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“…Liposomes with fusogenic properties [177] or that are pH sensitive have also been studied as drug carriers [178], given that they have the ability to destabilize endosomal membranes and release drugs into the cytoplasm [9]. The use of polyampholyte NPs and freeze concentration has been suggested to deliver proteins into the cytoplasm [174], as has the application of a faded electric treatment for siRNA delivery [179] via connectosomes and diffusion across TJs [180], bubble liposomes and ultrasound [181]. Enzyme-sensitive or redox-responsive linkers responsible for degradation of cell membrane have also been reported [7].…”

Section: Cytoplasmmentioning

confidence: 99%

Strategies for the enhanced intracellular delivery of nanomaterials

Azevedo

Macedo

Sarmento

2018

Drug Discovery Today

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The intracellular delivery of nanomaterials and drugs has been attracting increasing research interest, mainly because of their important effects and functions in several organelles. Targeting specific organelles can help treat or decrease the symptoms of diabetes, cancer, infectious, and autoimmune diseases. Tuning biological and chemical properties enables the creation of functionalized nanomaterials with enhanced intracellular uptake, ability to escape premature lysosome degradation, and to reach a specific target. Here, we provide an update of recent advances in the intracellular delivery mechanisms that could help drugs reach their target more efficiently.

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Faint electric treatment-induced rapid and efficient delivery of extraneous hydrophilic molecules into the cytoplasm

Cited by 24 publications

References 25 publications

Solid‐in‐oil nanodispersions for transdermal drug delivery systems

Solid‐in‐oil nanodispersions for transdermal drug delivery systems

The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment

Strategies for the enhanced intracellular delivery of nanomaterials

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