False positive results in cytotoxicity testing due to unexpectedly volatile compounds

Ciapetti, G.; Granchi, Donatella; Verri, E.; Savarino, L.; Stea, Susanna; Savioli, Franca; Gori, Alessandra; Pizzoferrato, Alberto

doi:10.1002/(sici)1097-4636(199802)39:2<286::aid-jbm16>3.0.co;2-b

Cited by 14 publications

(6 citation statements)

References 12 publications

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“…To investigate potential cytotoxic effects of larval presence, release of LDH into the culture medium was employed in brain slice and CNS cell co-cultures. Additionally, trypan blue staining were performed on CNS cell co-cultures as usage of more than one assay method has been recommended in cytotoxicity testing to avoid experimental artefacts (Ciapetti et al 1998). Trypan blue exclusion stain was chosen in addition to the LDH assay as sometimes simple, inexpensive manual techniques are less prone to interferences than enzymatic kits (Weyermann et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of Toxocara larvae on brain cell survival by in vitro model assessment

Heuer

Haendel

Beineke³

et al. 2015

Parasitology

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Neuroinvasive larvae of the common dog and cat roundworms, Toxocara canis and Toxocara cati, may cause severe neurological and neuropsychological disturbances in humans. Despite their pathogenic potential and high prevalence worldwide, little is known about their cell-specific influences and cerebral host-pathogen interactions in neurotoxocarosis. To address this discrepancy, a co-culture system of viable larvae with murine neuronal (CAD), oligodendrocytal (BO-1) and microglial (BV-2) cell lines has been established. Additionally, murine adult brain slices have been co-cultured with Toxocara larvae to consider complex organotypic cell-cell interplay. Cytotoxicity of larval presence was measured enzymatically and microscopically. Microscopic evaluation using trypan blue exclusion assay revealed to be less reliable and sensitive than the lactate dehydrogenase activity assay. Ultimately, even low numbers of both T. canis and T. cati larvae have impaired survival of differentiated CAD cells, which morphologically resemble primary neurons. In contrast, viability of oligodendrocytal and microglial cells as well as brain slices was not impaired by larval presence. Therefore, immune-mediated mechanisms or trauma by migrating larvae presumably induce the in vivo pathology rather than acute cytotoxic effects. Conclusively, the helminthic larvae co-culture system presented here is a valuable in vitro tool to study cell-specific effects of parasitic larvae and their products.

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Section: Discussionmentioning

confidence: 99%

Effects of Toxocara larvae on brain cell survival by in vitro model assessment

Heuer

Haendel

Beineke³

et al. 2015

Parasitology

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“…The potency of PCBs that are hydrophobic and have relatively high vapor pressure is difficult to assess in multiwell cell culture plates, such as those used in the E-SCREEN assay. This is due to volatilization that may induce false positive responses in nearby control wells (40). However, we were able to avoid this problem by using uncontaminated controls from concurrent estradiol control plates when potential control contamination on the PCB plates were identified.…”

Section: Discussionmentioning

confidence: 99%

Estrogenic Activity of Polychlorinated Biphenyls Present in Human Tissue and the Environment

deCastro

Korrick

Spengler

et al. 2006

Environ. Sci. Technol.

102

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This study evaluated the estrogenicity of polychlorinated biphenyls (PCBs) present in environmental media and human tissue and assessed exposure pathways for PCB-derived estrogenic potency in air, soil, and dust from New Bedford, MA, an area with a PCB-contaminated Superfund site. Thirty-four PCB congeners were assayed for estrogenic potency using E-SCREEN, an assay based on the estrogen-dependent proliferation of MCF-7 cells in vitro. Childhood exposure to estradiol-equivalents via PCBs in environmental media was estimated byweighting previously reported New Bedford congener-specific concentrations by their relative estrogenic potency and published inhalation and soil ingestion rates. Thirteen congeners were weakly estrogenic in E-SCREEN: PCBs 17, 18, 30, 44, 49, 66, 74, 82, 99, 103, 110, 128, and 179. These PCBs were typically 6 orders of magnitude less potent than 17beta-estradiol, with proliferative potencies ranging from 0.0007% to 0.0040%. Of the environmental media assessed, air (inhalation) had the highest PCB-derived estradiol-equivalent exposure. PCB estrogenic potency information from this study provides an important resource both for preliminary estimation of routes of human exposure to xenoestrogens and for application to human health studies focused on estrogen-responsive health outcomes, such as reproductive development and related malignancies.

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“…Its purpose is to act as a reliable, convenient, and reproducible screening method to detect, at an early stage in the testing process, cell death or other serious negative effects on cellular functions. [22][23][24][25][26][27][28][29] Cytotoxicity deals mainly with the substances that leach out of biomaterials. 30,31 For example, polymers often have low molecular weight 'leachables' (additives, low molecular weight components, initiator fragments, etc.)…”

Section: In Vitro Biocompatibility Screening: Types Of Test and Inter...mentioning

confidence: 99%

Biodegradable polymers and composites in biomedical applications: from catgut to tissue engineering. Part 1 Available systems and their properties

Gomes¹,

Reis²

2004

International Materials Reviews

106

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Biodegradable polymers form a unique class of materials that created an entirely new concept when originally proposed as biomaterials. That is, for the first time, a material performing a structural application was designed to be completely resorbed and to become weaker over time. This concept was first applied successfully with catgut sutures and later, with more arguable results, on bone fixation plates and pins. Current research on new and improved biodegradable polymers is focused on more sophisticated biomedical applications to solve patients' problems with higher efficacy and the least possible pain. One example is tissue engineering, in which a biodegradable scaffold seeded with an appropriate cell type provides a substitute for damaged human tissue while the natural process of regeneration is completed. An overview is given of the degradation properties and mechanisms of biodegradable polymers, their processability and biocompatibility, focusing on the aspects most relevant to biomedical applications. The main families of biodegradable polymeric systems are described and the systems that are commercially available or that are currently being studied and proposed for specific medical applications are reviewed.

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False positive results in cytotoxicity testing due to unexpectedly volatile compounds

Cited by 14 publications

References 12 publications

Effects of Toxocara larvae on brain cell survival by in vitro model assessment

Effects of Toxocara larvae on brain cell survival by in vitro model assessment

Estrogenic Activity of Polychlorinated Biphenyls Present in Human Tissue and the Environment

Biodegradable polymers and composites in biomedical applications: from catgut to tissue engineering. Part 1 Available systems and their properties

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