False positives in Biolog EcoPlatesTM and MT2 MicroPlatesTM caused by calcium

Pierce, Melissa L.; Ward, J. Evan; Dobbs, Fred C.

doi:10.1016/j.mimet.2013.12.002

Cited by 22 publications

(12 citation statements)

References 11 publications

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“…2 for sample distribution). Because Ca +2 concentrations greater than 100 ppm can induce false positives in EcoPlates (Pierce et al 2014), 1 sample was taken from each half core to determine the Ca content (see below).…”

Section: Ecoplate Aerobic Incubationsmentioning

confidence: 99%

Potential microbial functional activity along a Posidonia oceanica soil profile

Piñeiro‐Juncal¹,

Holmer

Cortizas³

2018

Aquat. Microb. Ecol.

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Among the ecosystem services provided by the seagrass Posidonia oceanica, its role as a carbon sink has received growing attention during the last decade. The sequestration and stabilization of carbon in soils are tightly linked to the activity of its microbial community. EcoPlates (Biolog) comprise a semi-quantitative tool for the assessment of microbial functional activity that generates profiles of microbial carbon source utilization. In this study, the aerobic and anaerobic carbon consumption in a healthy P. oceanica meadow was compared throughout depths down to 130 cm. After 11 d, the average well color development, Shannon-Wiener diversity index (H'), and kinetics of carbon consumption were assessed. Both the aerobic and anaerobic metabolisms showed carbon consumption at all depths. Color observations indicated that carbohydrates, amino acids and polymers were mainly consumed, while carboxylic acids, amines and miscellaneous compounds presented low or no consumption at all. Carbon consumption kinetics were similar for both metabolisms. The results suggest a pronounced stratification of the microbial community controlled by oxygen availability. Despite the higher aerobic metabolism in the top 40 cm, the anaerobic metabolism was dominant, supporting high sequestration of carbon in P. oceanica meadows. KEY WORDS: Biolog ® EcoPlates TM • Microbial activity • Aerobic metabolism • Anaerobic metabolism • Long-term carbon sink • Posidonia oceanica Resale or republication not permitted without written consent of the publisher This authors' personal copy may not be publicly or systematically copied or distributed, or posted on the Open Web, except with written permission of the copyright holder(s). It may be distributed to interested individuals on request.

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Section: Ecoplate Aerobic Incubationsmentioning

confidence: 99%

Potential microbial functional activity along a Posidonia oceanica soil profile

Piñeiro‐Juncal¹,

Holmer

Cortizas³

2018

Aquat. Microb. Ecol.

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“…It might be argued that the differences in substrate group utilization rates between sites might be due to the differences in cations such as Ca 21 , as calcium concentrations greater than 100 ppm can interfere with the microplate chemistry (i.e. presence of undissolved material and/or precipitation of the redox dye) and cause false positive readings (Pierce et al, 2014). The coastal site had significantly higher IC content and our sediment slurries were prepared with filtered seawater, however, we noticed no undissolved material or precipitation of the redox dye in the wells of our plates and there was no significant AWCD increase in our control wells over time.…”

Section: Bacterial Clpps Using Biolog Ecoplates Tmmentioning

confidence: 99%

“…However, little is known about carbon substrate utilization by heterotrophic bacteria inhabiting seagrass meadows or the role of these bacteria in carbon accumulation (Luna et al, 2013). In this study, we investigate carbon utilization patterns in heterotrophic bacterial communities in seagrass sediment cores from two sites in Western Australia using Biolog EcoPlates, which contains 31 carbon sources in triplicate (Garland and Mills, 1991) and has provided information on the functional diversity of heterotrophic bacteria in environmental samples (Garland and Mills, 1991;1996;Zak et al, 1994;Preston-Mafham et al, 2002;Sala et al, 2008;Pierce et al, 2014). Our two study sites, a coastal ocean site (Cockburn Sound) and an estuarine site (Oyster Harbour), had different sediment characteristics (e.g., percentage organic and inorganic carbon (IC), nitrogen and grain size fractions) thus we hypothesized that the differences in the carbon pool and sediment characteristics would select for distinct bacterial communities at the sites.…”

Section: Introductionmentioning

confidence: 99%

Utilization of carbon substrates by heterotrophic bacteria through vertical sediment profiles in coastal and estuarine seagrass meadows

Säwström

Serrano

Rozaimi

et al. 2016

Environ Microbiol Rep

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Coastal vegetated ecosystems play an important role in carbon cycling and bacterial communities inhabiting coastal sediments are responsible for the remineralization and processing of organic carbon (OC). We collected 1 m-long sediment cores in Posidonia seagrass meadows from coastal and estuarine sites in Australia that differed in their sedimentary organic and inorganic carbon, nitrogen and mud contents. The metabolic diversity of sediment heterotrophic bacterial communities was characterized at different sediment depths, based on the utilization pattern of 31 individual carbon substrates using Biolog EcoPlates . High metabolic diversity was recorded at both sites, but the carbon substrate utilization rates and the use of carbohydrates were higher at the coastal site compared to the estuarine site. The heterotrophic bacterial community in the coastal sediment appeared to metabolize a more diverse OC pool compared to the estuarine site, which might partly explain the differences in OC storage among the seagrass habitats studied. The Biolog EcoPlates provided a useful tool for characterising the sediment heterotrophic bacterial communities in the meadows and sediment characteristics and biochemical composition of the organic matter played an important role in shaping heterotrophic bacterial communities and their carbon utilization rates, potentially affecting carbon accumulation and preservation within seagrass sediments.

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“…of the soil microbial community [12][13][14]. It relies on the microbial inoculation extracted from the soil into microplates containing three replicate wells of 31 of the most useful carbon sources.…”

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confidence: 99%

Soil Microbial Functional Diversity Responses to Different Revegetation Types in Baishilazi Nature Reserve

Deng¹,

Zhou²,

Bai³

et al. 2019

Pol. J. Environ. Stud.

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Soil microorganisms play important roles in the dynamic regulation of organic matter in the forest ecosystem and are affected by different revegetation types. To reveal the influence of different revegetation types on soil microorganisms, we examined soil properties, soil microbial activity and diversity in Baishilazi Nature Reserve, including two planted coniferous forests (LG: Larix gmelinii, PK: Pinus koraiensis), two natural secondary broadleaf forests (JM: Juglans mandshurica, QM: Quercus mongolica), and one conifer-broadleaf forest (CB). Biolog-Eco plates were used to study soil microbial functional diversity. We found that the content of soil total C and total N existed higher under the broadleaf forests (JM, QM) than conifer-broadleaf forest (CB) and coniferous forests (LG, PK). Carbon source utilization capacity and soil microbial activity showed significant variations among different revegetation types. Soil microbial activity of natural secondary forests was significantly higher than planted coniferous forests, and JM created the highest soil microbial activity. Heatmap and PCA plot clearly differentiated among the different samples. The broadleaf forests, conifer-broadleaf forest and coniferous forests were well separated from each other, especially along the PC1, and the position of conifer-broadleaf forest was intermediate. The findings of canonical correspondence analysis (CCA) suggested that soil total C and total N were the main factors affecting soil microbial functional diversity. This study investigated how shifts in soil microbial functional diversity affected by different revegetation types were operational indicators of soil quality

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False positives in Biolog EcoPlatesTM and MT2 MicroPlatesTM caused by calcium

Cited by 22 publications

References 11 publications

Potential microbial functional activity along a Posidonia oceanica soil profile

Potential microbial functional activity along a Posidonia oceanica soil profile

Utilization of carbon substrates by heterotrophic bacteria through vertical sediment profiles in coastal and estuarine seagrass meadows

Soil Microbial Functional Diversity Responses to Different Revegetation Types in Baishilazi Nature Reserve

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