Utilization of carbon substrates by heterotrophic bacteria through vertical sediment profiles in coastal and estuarine seagrass meadows

Säwström, Christin; Serrano, Óscar; Rozaimi, Mohammad; Lavery, Paul S.

doi:10.1111/1758-2229.12406

Cited by 16 publications

(13 citation statements)

References 32 publications

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“…A similar suggestion was proposed by Oszust et al ( 2014 ). Despite the potential limitations of using Biolog EcoPlates™ connected with the fact that this method concerns microorganisms that are cultured on plates and may fail in the case of those uncultured in laboratory conditions (Zak et al 1994 ), our results may be supported by the findings reported by Säwström et al ( 2016 ) regarding subsurface seagrass sediments in the Mediterranean Sea. They noted faster carbon substrate utilization in a sandy coastal meadow than in a muddy one.…”

Section: Discussionsupporting

confidence: 80%

Microbial biodiversity of meadows under different modes of land use: catabolic and genetic fingerprinting

Wolińska

Frąc

Oszust

et al. 2017

World J Microbiol Biotechnol

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The main goal of the study was to find differences in the bacterial community structure resulting from different ways of meadow management in order to get the first insight into microbial biodiversity in meadow samples. The next generation sequencing technique (454-pyrosequencing) was accompanied with the community level physiological profiling (CLPP) method in order to acquire combined knowledge of both genetic and catabolic bacterial fingerprinting of two studied meadows (hayland and pasture). Soil samples (FAO: Mollic Gleysol) were taken in April 2015 from the surface layer (0–20 cm). Significant differences of the bacterial community structure between the two analyzed meadows resulted from different land mode were evidenced by pyrosequencing and CLPP techniques. It was found that Alpha- and Gammaproteobacteria dominated in the hayland, whereas Delta- and Betaproteobacteria prevailed in the pasture. Additionally, the hayland displayed lower Firmicutes diversity than the pasture. Predominant bacterial taxa: Acidobacteria, together with Chloroflexi and Bacteroidetes seemed to be insensitive to the mode of land use, because their abundance remained at a similar level in the both studied meadows. The CLPP analysis confirmed much faster degradation of the carbon sources by microorganisms from the hayland rather than from the pasture. Amino acids were the most favoured carbon source groups utilized by microorganisms in contrast to carbohydrates, which were utilized to the lowest extent. The study clearly proved that the consequences of even moderate anthropogenic management are always changes in bacterial community structure and their metabolic activity. Bacterial taxa that are sensitive and resistant on modes of land use were determined.Electronic supplementary materialThe online version of this article (doi:10.1007/s11274-017-2318-2) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 80%

Microbial biodiversity of meadows under different modes of land use: catabolic and genetic fingerprinting

Wolińska

Frąc

Oszust

et al. 2017

World J Microbiol Biotechnol

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“…The H' index was high and similar for both metabolisms along the depth of the core, suggesting a diverse microbial community, similar to previous findings in P. oceanica meadows (García-Martínez et al 2009, Säwström et al 2016 and over terrestrial soils (Liao et al 2016, Nurulita et al 2016, Thomas et al 2016.…”

Section: Stratificationsupporting

confidence: 88%

“…Similar studies, under aerobic conditions, found no consumption of one of the carboxylic acids (E3, γhydroxybutyric acid) and very low consumption of a miscellaneous compound (H2, D,L-α-glycerol phosphate) in peat samples (Pérez Rodríguez & Martínez Cortizas 2014); minimum consumption of amines, carboxylic acids and phenolic compounds (C3 and D3, here as carboxylic acids) in seagrass sediment cores from estuarine and coastal environments (Säwström et al 2016); and very low consumption of G3 and C3 (both carboxylic acids) and, again, no consumption of E3, in a large set of soil samples (Rutgers et al 2016), suggesting limited consumption of these carbon sources in soils.…”

Section: Substrate Consumptionmentioning

confidence: 72%

“…EcoPlates have been used to characterize microbial activity in Posi-donia spp. mats, in the water column above them (Richir et al 2012) and in the soil (Säwström et al 2016), but only under aerobic conditions. As the mat is a highly reduced environment, experiments comparing both aerobic and anaerobic activity may provide important information on the role of the 2 metabolisms.…”

Section: Introductionmentioning

confidence: 99%

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Potential microbial functional activity along a Posidonia oceanica soil profile

Piñeiro‐Juncal¹,

Holmer

Cortizas³

2018

Aquat. Microb. Ecol.

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Among the ecosystem services provided by the seagrass Posidonia oceanica, its role as a carbon sink has received growing attention during the last decade. The sequestration and stabilization of carbon in soils are tightly linked to the activity of its microbial community. EcoPlates (Biolog) comprise a semi-quantitative tool for the assessment of microbial functional activity that generates profiles of microbial carbon source utilization. In this study, the aerobic and anaerobic carbon consumption in a healthy P. oceanica meadow was compared throughout depths down to 130 cm. After 11 d, the average well color development, Shannon-Wiener diversity index (H'), and kinetics of carbon consumption were assessed. Both the aerobic and anaerobic metabolisms showed carbon consumption at all depths. Color observations indicated that carbohydrates, amino acids and polymers were mainly consumed, while carboxylic acids, amines and miscellaneous compounds presented low or no consumption at all. Carbon consumption kinetics were similar for both metabolisms. The results suggest a pronounced stratification of the microbial community controlled by oxygen availability. Despite the higher aerobic metabolism in the top 40 cm, the anaerobic metabolism was dominant, supporting high sequestration of carbon in P. oceanica meadows. KEY WORDS: Biolog ® EcoPlates TM • Microbial activity • Aerobic metabolism • Anaerobic metabolism • Long-term carbon sink • Posidonia oceanica Resale or republication not permitted without written consent of the publisher This authors' personal copy may not be publicly or systematically copied or distributed, or posted on the Open Web, except with written permission of the copyright holder(s). It may be distributed to interested individuals on request.

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“…Seagrass exudation is known to change with environmental conditions (e.g. light restriction) (43), and can act as an important resource for sediment microbes (26, 44). Our concomitant observations of belowground biomass loss in washed Z. marina plants and large-scale changes in the microbiome structure within the first week after transplantation may be related to changes in root exudation, and would suggest a rapid and coordinated response by both the microbiome and plant to disturbance.…”

Section: Discussionmentioning

confidence: 99%

Recovery and Community Succession of theZostera marinaRhizobiome After Transplantation

Wang

English

Tomàs

et al. 2020

Preprint

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18Seagrasses can form mutualisms with their microbiomes that facilitate the exchange of 19 energy sources, nutrients, and hormones, and ultimately impact plant stress resistance. Little is 20 known about community succession within the belowground seagrass microbiome after 21 disturbance and its potential role in the plant's recovery after transplantation. We transplanted 22 Zostera marina shoots with and without an intact rhizosphere and cultivated plants for four 23 weeks while characterizing microbiome recovery and effects on plant traits. Rhizosphere and 24 root microbiomes were compositionally distinct, likely representing discrete microbial niches. 25 Furthermore, microbiomes of washed transplants were initially different from those of sod 26 transplants, and recovered to resemble an undisturbed state within fourteen days. Conspicuously, 27 changes in microbial communities of washed transplants corresponded with changes in 28 rhizosphere sediment mass and root biomass, highlighting the strength and responsive nature of 29 the relationship between plants, their microbiome, and the environment. Potential mutualistic 30 microbes that were enriched over time include those that function in the cycling and turnover of 31 sulfur, nitrogen, and plant-derived carbon in the rhizosphere environment. These findings 32 highlight the importance and resiliency of the seagrass microbiome after disturbance. 33 Consideration of the microbiome will have meaningful implications on habitat restoration 34 practices. 35Importance 37 Seagrasses are important coastal species that are declining globally, and transplantation 38 can be used to combat these declines. However, the bacterial communities associated with 39 seagrass rhizospheres and roots (the microbiome) are often disturbed or removed completely 40 prior to transplantation. The seagrass microbiome benefits seagrasses through metabolite, 41 nutrient, and phytohormone exchange, and contributes to the ecosystem services of seagrass 42 meadows by cycling sulfur, nitrogen, and carbon. This experiment aimed to characterize the 43 importance and resilience of the seagrass belowground microbiome by transplanting Zostera 44 marina with and without intact rhizospheres and tracking microbiome and plant morphological 45 recovery over four weeks. We found the seagrass microbiome to be resilient to transplantation 46 disturbance, recovering after fourteen days. Additionally, microbiome recovery was linked with 47 seagrass morphology, coinciding with increases in rhizosphere sediment mass and root biomass. 48Results of this study can be used to include microbiome responses in informing future restoration 49 work. 52The rhizobiome has long been recognized to have important impacts on plant growth and 53 health (1). The microbes of the rhizobiome, which directly interact with and are influenced by 54 the roots (2), can benefit their plant hosts through recycling and producing bioavailable nutrients 55 (3-5), increasing disease resistance through competition with or in...

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Utilization of carbon substrates by heterotrophic bacteria through vertical sediment profiles in coastal and estuarine seagrass meadows

Cited by 16 publications

References 32 publications

Microbial biodiversity of meadows under different modes of land use: catabolic and genetic fingerprinting

Microbial biodiversity of meadows under different modes of land use: catabolic and genetic fingerprinting

Potential microbial functional activity along a Posidonia oceanica soil profile

Recovery and Community Succession of theZostera marinaRhizobiome After Transplantation

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