Farbstofffreie Zweiphotonenmikroskopie der Augenhornhaut

Krüger, Alexander; Hovakimyan, Marina; Ramírez, D. F.; Stachs, Oliver; Lubatschowski, Holger; Wree, Andreas; Guthoff, Rudolf; Heisterkamp, Alexander

doi:10.1055/s-0028-1109918

Cited by 2 publications

(1 citation statement)

References 35 publications

(41 reference statements)

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“…Because it is difficult to fully distinguish dECM from PLGA using macroscopic imaging and even nano-to-micrometer scale scanning electron images (Figure 3B and Figure S2 (Supporting Information)), innate second harmonic autofluorescence generated by collagen [44] was utilized to volumetrically map the topography and structure of each tissue paper (Figure 3C). Figure 3C shows collagen, due to its autofluorescence, and does not depict the interconnected PLGA network, which does not substantially autofluoresce.…”

Section: Resultsmentioning

confidence: 99%

“Tissue Papers” from Organ‐Specific Decellularized Extracellular Matrices

Jakus

Laronda

Rashedi

et al. 2017

Adv Funct Materials

110

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Using an innovative, tissue-independent approach to decellularized tissue processing and biomaterial fabrication, the development of a series of “tissue papers” derived from native porcine tissues/organs (heart, kidney, liver, muscle), native bovine tissue/organ (ovary and uterus), and purified bovine Achilles tendon collagen as a control from decellularized extracellular matrix particle ink suspensions cast into molds is described. Each tissue paper type has distinct microstructural characteristics as well as physical and mechanical properties, is capable of absorbing up to 300% of its own weight in liquid, and remains mechanically robust (E = 1–18 MPa) when hydrated; permitting it to be cut, rolled, folded, and sutured, as needed. In vitro characterization with human mesenchymal stem cells reveals that all tissue paper types support cell adhesion, viability, and proliferation over four weeks. Ovarian tissue papers support mouse ovarian follicle adhesion, viability, and health in vitro, as well as support, and maintain the viability and hormonal function of nonhuman primate and human follicle-containing, live ovarian cortical tissues ex vivo for eight weeks postmortem. “Tissue papers” can be further augmented with additional synthetic and natural biomaterials, as well as integrated with recently developed, advanced 3D-printable biomaterials, providing a versatile platform for future multi-biomaterial construct manufacturing.

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Section: Resultsmentioning

confidence: 99%