Fascin 1 is transiently expressed in mouse melanoblasts during development and promotes migration and proliferation

Ma, Yafeng; Li, Ang; Faller, William J.; Libertini, Silvana; Fiorito, Florencia; Gillespie, D. A. F.; Sansom, Owen J.; Yamashiro, Shigeko; Machesky, Laura M.

doi:10.1242/jcs.135186

Cited by 12 publications

(19 citation statements)

References 47 publications

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“…By stage 28, the distribution of melanoblasts in embryos resembles that in adults [5]. Recently, a few signal molecules that can enhance or constrain melanoblast migration have been identified [6][7][8][9][10], but the molecular mechanisms responsible for the migration of melanoblasts requires further investigation.…”

Section: Introductionmentioning

confidence: 99%

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl

Koltes¹,

Deng²,

Li³

et al. 2014

Front. Agr. Sci. Eng.

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Melanoblasts originating from neural crest cells can migrate through the mesenchyme of the developed embryo and give rise to melanocytes. Unlike the melanocytes that are confined to the integument in other vertebrates, melanocytes in Silky Fowl can reach the ventral regions of the embryos owing to differences in gene expression in the process of melanoblasts migration. In this study, we used microarray profiling to identify differences in gene expression between White Leghorn and Silky Fowl. Differential expression of 2517 microarray probes (P < 0.01, Fold Change > 2) was observed in Silky Fowl compared to White Leghorn. After filtration by cluster analysis, functional annotation and pathway analysis, eight differentially expressed genes were identified to be closely related to the development of melanocytes. Moreover, differences in expression of immune genes were also detected between Silky Fowl and White Leghorn. The differentially expressed genes associated with melanocyte development were verified by q-PCR, and results were highly consistent with the microarray data. The genes with significantly altered expression involved in melanoblast migration and development suggested that different microenvironments resulted in the abnormal melanoblast migration in Silky Fowl, although there were no big differences in melanoblast development between these two breeds. The candidate genes discovered in this study are beneficial to understand the molecular mechanism of hyperpigmentation in Silky Fowl.

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Section: Introductionmentioning

confidence: 99%

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl

Koltes¹,

Deng²,

Li³

et al. 2014

Front. Agr. Sci. Eng.

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“…Fascin has three isoforms in mammals: fascin1, which is widely expressed, fascin2, which is expressed in retina and ear hair cells and fascin3 which is specific to testis . In adult mammals, fascin1 is expressed mainly in neuronal cells, endothelial cells, fibroblasts, smooth muscle cells, dendritic cells and some motile embryonic cells (neuroblasts and melanoblasts). In normal epithelial tissue, fascin1 is absent or present at low levels.…”

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confidence: 99%

Fascin1 in carcinomas: Its regulation and prognostic value

Machesky

2014

Intl Journal of Cancer

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Previous cell biological studies demonstrate that the actin bundling protein fascin1 regulates cell motility, migration and invasion. Human studies demonstrate that fascin1 is upregulated in many epithelial cancers. This review gives a brief overview of the role of fascin1 in cell migration and invasion, but focuses mainly on the regulation and clinical relevance of fascin1 in epithelial cancers. Here, we propose fascin1 as a potent prognostic biomarker for breast, colorectal, esophageal cancers and head and neck squamous cell carcinomas. Fascin1 may also be an attractive drug target against these carcinomas in the future, but more studies are needed.Epithelial cancers (carcinomas) comprise 80-90% cancer types. Although early tumor diagnosis and improved multimodality therapy have helped to reduce the mortality of cancer patients, prognosis remains poor if the cancer is invasive and metastatic. Fascin was first discovered as a 55 kDa actinassociated protein in the filopodia of coelomocytes in sea urchin. 1 Fascin has three isoforms in mammals: fascin1, which is widely expressed, fascin2, which is expressed in retina and ear hair cells and fascin3 which is specific to testis. 2 In adult mammals, fascin1 is expressed mainly in neuronal cells, 3,4 endothelial cells, 5 fibroblasts, 6 smooth muscle cells, 7 dendritic cells 8 and some motile embryonic cells (neuroblasts 9 and melanoblasts 10 ). In normal epithelial tissue, fascin1 is absent or present at low levels. However, fascin1 is frequently upregulated when epithelial tissues become malignant. [11][12][13] This review focuses on the recent advances in the regulation of fascin1 in cancer and summarizes the existing clinical studies in various types of cancers to reveal its prognostic significance. Recent literature suggests that the cell migration promoting protein fascin1 could serve as a prognostic marker in multiple types of carcinomas, including breast, colorectal, esophageal cancers and head and neck squamous cell carcinomas (HNSCC). Actin Bundling Protein Fascin1: A Key Player in Cell Migration and InvasionFascin exists in equilibrium between the cytoplasm and the cytoskeleton and its association with actin filaments is enhanced by interaction with specific matrix 14 or stimulation of cells with growth factors or cytokines. 15 Fascin1 has at least two binding sites for actin filaments, so it crosslinks them into tightly packed parallel bundles, oriented with their growing ends toward the plasma membrane. Migration is driven by a combination of actin polymerization dynamics underneath the plasma membrane, adhesion to the substratum and contractile forces generated by actin-myosin structures. Fascin is found in actin-rich cytoskeletal structures, such as stress fibers, 16,17 protrusive membrane structures (microspikes and filopodia 18 ) and degradative structures (invadopodia 19,20 and podosome 7 ) (Fig. 1a) which are all pro-migratory. Previous cell biological studies have revealed that fascin1 executes key roles in these structures and that cells expressi...

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“…Some of these genes are also associated with the neural crest and melanocyte disorders in humans. Example of these genes include the transcription factors: Mitf [16][17][18], Sox10 [19,20]; Receptor tyrosine kinase Kit and its ligand KitL [21][22][23][24]; G-protein coupled endothelin-3 receptor/ligand pair [25,26]; Notch signaling [27]; small Rho GTPase Rac1 [28] and its activator P-Rex1 [29]; and actin bundling protein Fascin1 [30][31][32] and secreted protease of the ADAM family of the metalloproteinase ADAMTS20 [33].…”

Section: Melanocyte Developmentmentioning

confidence: 99%

“…[30,31]. Interestingly, fascin expression is transiently upregulated in the melanoblast and unregulated in the melanocytes [32], suggesting that fascin is required for efficient melanoblast migration. Indeed, in fascin-deficient mice, the melanoblasts migrate slowly and form less longer pseudopods [32].…”

Section: Melanocyte Developmentmentioning

confidence: 99%

The biology of melanocyte and melanocyte stem cell

Li¹

2014

ABBS

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The melanocyte stem cells of the hair follicle provide an attractive system for the study of the stem cells. Successful regeneration of a functional organ relies on the organized and timely orchestration of molecular events among distinct stem/progenitor cell populations. The stem cells are regulated by communication with their specialized microenvironment known as the niche. Despite remarkable progress in understanding stem cell-intrinsic behavior, the molecular nature of the extrinsic factors provided to the stem cells by the niche microenvironment remains poorly understood. In this regard, the bulge niche of the mammalian hair follicle offers an excellent model for study. It holds two resident populations of SCs: epidermal stem cells and melanocyte stem cells. While their behavior is tightly coordinated, very little of the crosstalk involved is known. This review summarized the recent development in trying to understand the regulation of melanocyte and melanocyte stem cells. A better understanding of the normal regulation and behaviors of the melanocytes and the melanocyte stem cells will help to improve the clinical applications in regenerative medicine, cancer therapy, and aging.

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Fascin 1 is transiently expressed in mouse melanoblasts during development and promotes migration and proliferation

Cited by 12 publications

References 47 publications

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl

Fascin1 in carcinomas: Its regulation and prognostic value

The biology of melanocyte and melanocyte stem cell

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