Fasciola hepatica: Tegumental surface changes in adult and juvenile flukes following treatment in vitro with the sulphoxide metabolite of triclabendazole (Fasinex)

Stitt, Alan W.; Fairweather, Ian

doi:10.1007/bf00932235

Cited by 74 publications

(62 citation statements)

References 45 publications

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“…Similar observations were made for other anthelmintic drugs (Lee et al, 1987;Stitt & Fairweather, 1993;Apinhasmit & Sobhon, 1996;Meaney et al, 2002Meaney et al, , 2003Buchanan et al, 2003). Blebbing and swelling are early changes leading to loss of the tegument.…”

Section: Discussionsupporting

confidence: 74%

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

et al. 2007

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The effect of niclosamide on the tegument of adult Haplorchis taichui (Trematoda: Heterophyidae) exposed in vitro was observed by scanning electron microscope. Adult worms were incubated in Tyrode's solution containing 0.01, 0.1, 1.0, and 10 mg ml 21 of niclosamide for 30 min, 1, 6, 12 and 24 h. Control groups were incubated in Tyrode's solution without niclosamide and worms remained active until 24 h. In 0.01 mg ml 21 of niclosamide, worms showed slightly active movements up to 1 h after incubation, while in 0.1 mg ml 21 solution a few worms showed only slightly active movements after 30 min. Tegumental changes were determined by scanning electron microscopy. Swelling and blebbing of the tegument were observed on both ventral and dorsal sides. After longer periods, extensive swelling and blebbing of the tegument became more severe and there was a loss of the apical plasma membrane in some regions. Empty spine sockets occurred, and small perforations penetrated the basal lamina, followed by some lesions. Destruction of both surfaces was more pronounced on the posterior compared with the anterior regions.

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Section: Discussionsupporting

confidence: 74%

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

et al. 2007

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“…In Fasciola hepatica, a similar pattern of tegumental swelling had been described following incubation with a number of anthelmintics, as summarized by Hegazi et al [15]. This was a common feature of drug treated parasites and considered to be an indicator of a stress reaction by the parasite, in an attempt to replace damaged surface membrane [16].…”

Section: Discussionmentioning

confidence: 69%

Body Surface Changes in Gastrointestinal Helminthes Following in vitro Treatment with Allium sativum Oil

Shalaby¹

2014

J Veterinar Sci Technol

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A scanning electron microscopic study was undertaken to assess, for the first time, whether Allium sativum oil (ASO) extract had any effect on helminthes' body surface following 24 h incubation in vitro. Two species of gastrointestinal helminthes, of most economic significance in sheep, Haemonchus contortus and Moniezia expansa were collected from naturally infected sheep slaughtered in Cairo abattoir, then exposed to 10, 50, 100 µg/ml ASO. The body surface of these helminthes was observed to be affected and altered by ASO. These changes were concentration dependent, and consisted of destructive alterations and deformity in the cuticle of H. contortus and also in the tegumental architecture of M. expansa. The current study confirmed that an alternative, effective and natural anthelmintic could be developed using garlic. Body Surface Changes in Gastrointestinal Helminthes Following in vitro Treatment with Allium sativum OilHatem A Shalaby* and Tarek K Farag Department of Parasitology and Animal Diseases, National Research Center, Giza, Egypt Materials and Methods Preparation of A. sativum oil extractBulbs of fully grown A. sativum cultivated locally were used in the study. The dried bulbs were pounded and then steam distilled. The essential oil was extracted using solvent ether.

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“…Greater swelling of the basal infolds would lead to the sloughing of the tegument, as has been observed following treatment with other fasciolicides (e.g. Fairweather et al 1986 ;Skuce and Fairweather, 1990 ;Stitt and Fairweather, 1993 ;Anderson and Fairweather, 1995 ;Meaney et al 2004). Clearly, there is disruption of the tegumental osmoregulatory system, which spreads internally to affect the subtegumental tissues.…”

Section: Discussionmentioning

confidence: 93%

“…Fairweather et al 1987 ;Skuce and Fairweather, 1990 ;Stitt and Fairweather, 1993 ;McKinstry et al 2003 ; McConville et al 2006 ;Halferty et al 2009). More severe changes were seen in 2 of the flukes.…”

Section: Discussionmentioning

confidence: 99%

Electron microscopical study to assess thein vitroeffects of the synthetic trioxolane OZ78 against the liver fluke,Fasciola hepatica

et al. 2009

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Adult Fasciola hepatica were incubated for 48 h in vitro in the synthetic peroxide, OZ78 at a concentration of 100 mg/ml and then prepared for scanning and transmission electron microscopy. There was limited disruption to the external fluke surface, with only slight swelling and blebbing of the interspinal tegument in the midbody and ventral tail regions. By contrast, significant disruption was observed to the ultrastructure of the tegument and subtegumental tissues. There was severe swelling of the basal infolds in the tegumental syncytium and the flooding spread internally to affect the subtegumental tissues. In the tegumental system, there was swelling of the cisternae of granular endoplasmic reticulum and of the mitochondria, with the latter showing signs of breaking down. Autophagic vacuoles and lipid droplets were present and the synthesis of tegumental secretory bodies was much reduced. The gastrodermal cells were severely affected, with swelling and degeneration of the mitochondria and the presence of autophagic vacuoles and lipid droplets. The granular endoplasmic reticulum was swollen and vesiculated and the cells contained few secretory bodies. Both the vitelline and testis follicles showed evidence of extensive cellular disruption and degeneration. This study confirms previous data indicating the potential flukicidal activity of OZ78.

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Fasciola hepatica: Tegumental surface changes in adult and juvenile flukes following treatment in vitro with the sulphoxide metabolite of triclabendazole (Fasinex)

Cited by 74 publications

References 45 publications

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

Body Surface Changes in Gastrointestinal Helminthes Following in vitro Treatment with Allium sativum Oil

Electron microscopical study to assess thein vitroeffects of the synthetic trioxolane OZ78 against the liver fluke,Fasciola hepatica

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