Fast accurate quantification of salivary cortisol and cortisone in a large-scale clinical stress study by micro-UHPLC-ESI-MS/MS using a surrogate calibrant approach

Aydin, Ece; Drotleff, Bernhard; Noack, Hannes; Derntl, Birgit; Lämmerhofer, Michael

doi:10.1016/j.jchromb.2021.122939

Cited by 8 publications

(2 citation statements)

References 56 publications

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“…Another way to investigate the RF is to compare the slope parallelism between the authentic analyte and the surrogate, both performed in the same pooled matrix. Aydin et al demonstrated that the slope ratio between cortisol/cortisol-d 4 and cortisone/cortisone-13 C 3 was sufficiently close to the unit value over different days in human saliva, covering a dynamic range between 0.062 and 75.5 ng mL − 1 for cortisol and cortisone quantification, respectively [112]. Additionally, if the RF is not constant over the tested dynamic range measurement corrections, such as LC gradient or MS/MS transitions (de)-optimization, can be evaluated to obtain a balanced response [113][114][115].…”

Section: Surrogate Analyte(s) In Authentic Matrixmentioning

confidence: 92%

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

Visconti

Bernard

Feinberg

et al. 2023

Analytica Chimica Acta

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Section: Surrogate Analyte(s) In Authentic Matrixmentioning

confidence: 92%

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

Visconti

Bernard

Feinberg

et al. 2023

Analytica Chimica Acta

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“…Cortisol is commonly monitored by classical methods of analysis such as chromatography coupled to mass spectrometry, radioimmunoassay, chemiluminescent immunoassay, enzyme-linked immunosorbent assay (ELI-SA), surface plasmon resonance (SPR), quartz crystal microbalance (QCM) and ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) [6][7][8][9][10]. These methods are well-established and provide high sensitivity and reliability of the data generated.…”

Section: Introductionmentioning

confidence: 99%

A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

2023

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Cortisol is a steroid hormone naturally produced by the adrenal glands. It participates in and controls several processes in the body and is considered an important physiological biomarker. Due to its very low concentrations in body fluids, its detection requires high sensitivity and specificity. Here, we present a simple electrochemical biosensor based on reduced graphene oxide (rGO) modified with gold nanoparticles (AuNPs) for the immobilization of the cortisol‐specific aptamer (Ap) (MCH‐Ap‐AurGO/GCE). Important analytical parameters for identifying the target analyte were optimized, such as conditions and amount of immobilized Ap, the influence of the concentration and nature of the supporting electrolyte, pH of the medium, and incubation time. The optimized conditions for the aptasensor were: concentration of Ap 1.0 × 10−6 mol L−1, support electrolyte Tris/HCl 50 mmol L−1, MgCl2 10 mmol L−1, and NaCl 10 mmol L−1, at pH 5.0 and incubation time of 15 min. A linear response range was obtained from 1 × 10−18 up to 1 × 10−11 mol L−1 of cortisol with a detection limit (LOD) of 1.0 × 10−18 mol L−1. A curve adjusted for operational purposes in the saliva sample was fitted for the concentration range between 0.5 × 10−14 and 1 × 10−11 mol L−1, with a linear regression equation ΔRtc/Rtc1 = 2.70 + 0.17 × log([Cortisol]). The aptasensor demonstrated a great potential for detecting cortisol in a simple, fast, and highly sensitive way, opening its path for application in real samples, which present levels below the concentration in which cortisol is commonly found in body fluids.

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Hyphenation of microflow chromatography with electrospray ionization mass spectrometry for bioanalytical applications focusing on low molecular weight compounds: A tutorial review

Girel,

Meister,

Glauser

et al. 2024

Mass Spectrometry Reviews

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Benefits of miniaturized chromatography with various detection modes, such as increased sensitivity, chromatographic efficiency, and speed, were recognized nearly 50 years ago. Over the past two decades, this approach has experienced rapid growth, driven by the emergence of mass spectrometry applications serving ‐omics sciences and the need for analyzing minute volumes of precious samples with ever higher sensitivity. While nanoscale liquid chromatography (flow rates <1 μL/min) has gained widespread recognition in proteomics, the adoption of microscale setups (flow rates ranging from 1 to 100 μL/min) for low molecular weight compound applications, including metabolomics, has been surprisingly slow, despite the inherent advantages of the approach. Highly heterogeneous matrices and chemical structures accompanied by a relative lack of options for both selective sample preparation and user‐friendly equipment are usually reported as major hindrances. To facilitate the wider implementation of microscale analyses, we present here a comprehensive tutorial encompassing important theoretical and practical considerations. We provide fundamental principles in micro‐chromatography and guide the reader through the main elements of a microflow workflow, from LC pumps to ionization devices. Finally, based on both our literature overview and experience, illustrated by some in‐house data, we highlight the critical importance of the ionization source design and its careful optimization to achieve significant sensitivity improvement.

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Fast accurate quantification of salivary cortisol and cortisone in a large-scale clinical stress study by micro-UHPLC-ESI-MS/MS using a surrogate calibrant approach

Cited by 8 publications

References 56 publications

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

Hyphenation of microflow chromatography with electrospray ionization mass spectrometry for bioanalytical applications focusing on low molecular weight compounds: A tutorial review

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