Fast acting hemostatic agent based on self-assembled hybrid nanofibers from chitosan and casein

Mishra, Balaram; Hossain, Sabir; Mohanty, Sibanwita; Gupta, Mukesh Kumar; Verma, Devendra

doi:10.1016/j.ijbiomac.2021.06.116

Cited by 19 publications

(7 citation statements)

References 45 publications

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“…The test results of hemostasis in vitro and in vivo indicated that due to a better hemostatic effect, the hemostasis time of the composite was significantly reduced compared with that of the medical gauze on the market. This was probably because of the high density of positive charge of CTS that can interact with the receptors containing cytosine residues on the surface of red blood cells, thus causing the red blood cells to coagulate on the material surface to form a thrombus [ 32 , 33 ]. Furthermore, CTS can also adsorb negatively charged plasma proteins and adhere to and activate platelets through electrostatic force, which allows platelets and other blood substances to gather on the surface to form a thrombus, thus playing a blood coagulation role [ 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

et al. 2023

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Considering the great harm to the human body caused by severe and massive bleeding, in this study, chitosan-grafted norfloxacin (CTS-NF) composites were prepared with chitosan (CTS) and norfloxacin (NF) as raw materials by a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-mediated coupling method to solve the limitations of slow hemostatic and poor anti-infective effects of current dressings on the market. The effects of the mass ratio of CTS to NF (MCTS/MNF), reaction temperature T and reaction time t on the grafting rate (η%) of the products were investigated through single factor tests. The preparation process was optimized with the η% as an evaluation index by means of the Box–Behnken test design and response surface analysis. The antimicrobial activity was evaluated by inhibition zone assay, and the hemostatic activity of the prepared composites was evaluated in vitro and in vivo. The results suggested that the optimum preparation conditions were the mass ratio of CTS to NF( MCTS/MNF) 5:3, reaction temperature 65 °C, and reaction time 4 h. Under this condition, the η% of CTS-NF was 45.5%. The CTS-NF composites displayed significant antimicrobial activities. Moreover, in vitro hemostasis results revealed that the CTS-NF composite had a lower blood clotting index and absorbed red blood cells to promote aggregation. In vivo ear and live hemostasis, the CTS-NF groups showed short hemostatic time (49.75 ± 3.32 s and 50.00 ± 7.21 s) and more blood loss(0.07 ± 0.010 g and 0.075 ± 0.013 g). The results showed that CTS-NF reduced the bleeding time and volume, exhibiting a significant coagulation effect. Therefore, the CTS-NF sponge is expected to be a new, effective hemostatic and antibacterial material in the future.

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Section: Resultsmentioning

confidence: 99%

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

et al. 2023

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“…Preparation of the nanofibers followed the procedure from a previous report. 24 , 29 Briefly, PVA, IKVAV, or RGD was added to a mixture of 0.1 M NaOH and distilled water. The mixture was then incubated at 37 °C for 30 min and stirred vigorously until a clear liquid was formed.…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo Evaluation of the Bioactive Nanofibers-Encapsulated Benzalkonium Bromide for Accelerating Wound Repair with MRSA Skin Infection

Ran¹,

Peng²,

Wang³

et al. 2022

IJN

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Purpose Developing the ideal drug or dressing is a serious challenge to controlling the occurrence of antibacterial infection during wound healing. Thus, it is important to prepare novel nanofibers for a wound dressing that can control bacterial infections. In our study, the novel self-assembled nanofibers of benzalkonium bromide with bioactive peptide materials of IKVAV and RGD were designed and fabricated. Methods Different drug concentration effects of encapsulation efficacy, swelling ratio and strength were determined. Its release profile in simulated wound fluid and its cytotoxicity were studied in vitro. Importantly, the antibacterial efficacy, inhibition of biofilm formation effect and wound healing against MRSA infections in vitro and in vivo were performed after observing the tissue toxicity in vivo. Results It was found that the optimized drug load (0.8%) was affected by the encapsulation efficacy, swelling ratio, and strength. In addition, the novel nanofibers with average diameter (222.0 nm) and stabile zeta potential (−11.2 mV) have good morphology and characteristics. It has a delayed released profile in the simulated wound fluid and good biocompatibility with L929 cells and most tissues. Importantly, the nanofibers were shown to improve antibacterial efficacy, inhibit biofilm formation, and lead to accelerated wound healing following infection with methicillin-resistant Staphylococcus aureus . Conclusion These data suggest that novel nanofibers could effectively shorten the wound-healing time by inhibiting biofilm formation, which make it promising candidates for treatment of MRSA-induced wound infections.

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“…Carrageenan can be combined with lignin and other materials for application in drug delivery and food packaging (Numata et al, 2021;Rukmanikrishnan et al, 2020). Casein can be combined with alginate and chitosan to form nano-fibers and scaffold for applications in tissue engineering and drug delivery (Du et al, 2020;Mishra et al, 2021). Sodium carboxy-methyl xanthan gum can be combined with N-trimethyl chitosan to form hydrogel for drug delivery applications (Hanna and Saad, 2019).…”

Section: Biopolymer Blends and Compositesmentioning

confidence: 99%

“…A combination of Chit amine group and casein phosphate group was used to form a hybrid fiber. The produced material exhibited good properties such as hemostatic and enhanced mechanical properties (Mishra et al, 2021).…”

Section: Biopolymer Hybrid Systemsmentioning

confidence: 99%

Current state of the art biotechnological strategies for conversion of watermelon wastes residues to biopolymers production: A review

et al. 2022

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Fast acting hemostatic agent based on self-assembled hybrid nanofibers from chitosan and casein

Cited by 19 publications

References 45 publications

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

In vitro and in vivo Evaluation of the Bioactive Nanofibers-Encapsulated Benzalkonium Bromide for Accelerating Wound Repair with MRSA Skin Infection

Current state of the art biotechnological strategies for conversion of watermelon wastes residues to biopolymers production: A review

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