In vitro and in vivo Evaluation of the Bioactive Nanofibers-Encapsulated Benzalkonium Bromide for Accelerating Wound Repair with MRSA Skin Infection

Abstract

Purpose Developing the ideal drug or dressing is a serious challenge to controlling the occurrence of antibacterial infection during wound healing. Thus, it is important to prepare novel nanofibers for a wound dressing that can control bacterial infections. In our study, the novel self-assembled nanofibers of benzalkonium bromide with bioactive peptide materials of IKVAV and RGD were designed and fabricated. Methods Different drug concentration effects of encapsulation efficacy, swelling ratio and strength were determined. Its release profile in simulated wound fluid and its cytotoxicity were studied in vitro. Importantly, the antibacterial efficacy, inhibition of biofilm formation effect and wound healing against MRSA infections in vitro and in vivo were performed after observing the tissue toxicity in vivo. Results It was found that the optimized drug load (0.8%) was affected by the encapsulation efficacy, swelling ratio, and strength. In addition, the novel nanofibers with average diameter (222.0 nm) and stabile zeta potential (−11.2 mV) have good morphology and characteristics. It has a delayed released profile in the simulated wound fluid and good biocompatibility with L929 cells and most tissues. Importantly, the nanofibers were shown to improve antibacterial efficacy, inhibit biofilm formation, and lead to accelerated wound healing following infection with methicillin-resistant Staphylococcus aureus . Conclusion These data suggest that novel nanofibers could effectively shorten the wound-healing time by inhibiting biofilm formation, which make it promising candidates for treatment of MRSA-induced wound infections.

Nanofiber Peptides for Bacterial Trapping: A Novel Approach to Antibiotic Alternatives in Wound Infections

“…Over the preceding decades, the healthcare sector has faced persistent challenges from drug-resistant bacterial infections, thereby constraining treatment options. [52] The skin represents the largest stratified organ within the body, [53] and MRSA emerges as a predominant pathogen inciting wound infections postsurgery. [54] Given the resistance of MRSA to several antibiotics, including vancomycin, innovative antimicrobials such as AMPs have emerged as the cornerstone for treating wound infections attributed to MRSA.…”

Antibacterial–Antioxidative Thiolated Gelatin/Methacrylated Silk Fibroin Hydrogels with Nitric Oxide Release Catalyzed by Metal–Polyphenol Nanoparticles for MRSA-Infected Wound Healing

“…To treat wound infections, a variety of wound dressings, antimicrobial agents, and their combinations are developed and made available. − However, the limitations of tissue integrity, bioactive factor concentration, and antibiotic-resistance microorganisms have led to the search for suitable wound dressings that control bacterial infections and promote wound healing. , A hydrogel dressing is a promising alternative candidate because it can isolate or inhibit external bacteria, absorb extradition tissue exudate, and act as a drug carrier. Although hydrogel dressings provide a more moist environment for the wound bed, which benefits tissue repair, biofilms form more easily because bacteria have high growth vitality and tend to colonize and breed in humid environments, especially in the case of multidrug-resistant bacteria. − Therefore, it is desired to prepare multifunctional hydrogel dressings with excellent inhibition of wound infection formation and simultaneously promote satisfactory wound healing.…”