Fast and effective photodynamic inactivation of 4-day-old biofilm of methicillin-resistant Staphylococcus aureus using methylene blue-conjugated gold nanoparticles

Darabpour, Esmaeil; Kashef, Nasim; Amini, Seyed Mohammad; Kharrazi, Sharmin; Djavid, Gholamreza Esmaeeli

doi:10.1016/j.jddst.2016.12.007

Cited by 50 publications

(30 citation statements)

References 30 publications

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“…Four day-old biofilms of MRSA were treated with MB-conjugated GNPs and subsequently exposed to red light. MB-conjugated GNPs showed significant photo-inactivation against 4-day-old biofilm of MRSA (>5 log 10 CFU reduction) while free MB-APDI resulted in less than 1 log10 CFU reduction [142]. …”

Section: Biofilm Resistance To Apdimentioning

confidence: 99%

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Kashef

Hamblin

2017

Drug Resistance Updates

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255

173

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Infections have been a major cause of disease throughout the history of humans on earth. With the introduction of antibiotics, it was thought that infections had been conquered. However, bacteria have been able to develop resistance to antibiotics at an exponentially increasing rate. The growing threat from multi-drug resistant organisms calls for intensive action to prevent the emergence of totally resistant and untreatable infections. Novel, non-invasive, non-antibiotic strategies are needed that act more efficiently and faster than current antibiotics. One promising alternative is antimicrobial photodynamic inactivation (APDI), an approach that produces reactive oxygen species when dyes and light are combined. So far, it has been questionable if bacteria can develop resistance against APDI. This review paper gives an overview of recent studies concerning the susceptibility of bacteria towards oxidative stress, and suggests possible mechanisms of the development of APDI-resistance that should at least be addressed. Some ways to potentiate APDI and also to overcome future resistance are suggested.

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Section: Biofilm Resistance To Apdimentioning

confidence: 99%

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Kashef

Hamblin

2017

Drug Resistance Updates

Self Cite

255

173

View full text Add to dashboard Cite

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“…Then, 0.5 ml solution of fresh and ice-cold sodium borohydride (0.1 M) was injected to the above solution and stirred vigorously for another 15 min. Synthesis of 10 nm citrate-coated GNPs has been carried out based on our previous reports [31][32][33].…”

Section: Synthesis Of Gnpsmentioning

confidence: 99%

Radiofrequency electric field hyperthermia with gold nanostructures: role of particle shape and surface chemistry

Amini

Rezayat

Gilani

2017

Artificial Cells, Nanomedicine, and Biotechnology

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Hyperthermia treatment of cancerous cells has been recently developed drastically with the help of nanostructures. Heating of gold nanoparticles in non-invasive radiofrequency electric field (RF-EF) is a promising and unique technique for cancer hyperthermia. However, because of differences between particles (i.e. their surface chemistry and dispersion medium) and between RF-EF sources, the research community has not reached a consensus yet. Here, we report the results of investigations on heating of gold nanoparticles and gold nanorods under RF-EF and feasibility of in-vitro cancer hyperthermia. The heating experiments were performed to investigate the role of particle shape and surface chemistry (CTAB, citrate and PEG molecules). In-vitro hyperthermia was performed on human pancreatic cancer cell (MIA Paca-2) with PEG-coated GNPs and GNRs at concentrations that were found non-toxic based on the results of cytotoxicity assay. Application of RF-EF on cells treated with PEG-GNPs and PEG-GNRs proved highly effective in killing cells.

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“…The biofilm‐forming ability of the S. aureus strains were classified according to Darabpour et al . (): strong biofilm producer (OD 570 >4), moderate biofilm producer (2 ≤ OD 570 ≤ 4) and weak biofilm producer (OD 570 <2). All strains were cultured in tryptic soy broth (TSB) (Merck, Darmstadt, Germany) at 37°C for 20 h and maintained at −80°C in 20% (v/v) glycerol (Merck) for long‐term preservation.…”

Section: Methodsmentioning

confidence: 99%

“…The strains that were used in this study were S. aureus ATCC 6538P (methicillin susceptible, strong biofilm former), S. aureus ATCC 43300 (methicillin susceptible, moderate-strong biofilm former), S. aureus ATCC 33591 (methicillin susceptible, weak-moderate biofilm former) and S. aureus ATCC 29213 (methicillin susceptible, weak-moderate biofilm former). The biofilm-forming ability of the S. aureus strains were classified according to Darabpour et al (2017): strong biofilm producer (OD 570 >4), moderate biofilm producer (2 ≤ OD 570 ≤ 4) and weak biofilm producer (OD 570 <2). All strains were cultured in tryptic soy broth (TSB) (Merck, Darmstadt, Germany) at 37°C for 20 h and maintained at À80°C in 20% (v/v) glycerol (Merck) for long-term preservation.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Dimethyl methylene blue assay Dimethyl methylene blue (DMMB) assay was performed to evaluate the effects of a-tocopherol on the biofilm matrix of S. aureus, as according to Darabpour et al (2017). DMMB forms a complex with the polysaccharides in the biofilm matrix, which can be measured spectrophotometrically to give an indirect amount of the biofilm matrix.…”

Section: Characterization Of the Biofilm Disruption Effect Of A-tocopmentioning

confidence: 99%

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Identification of α-tocopherol as a bioactive component ofDicranopteris lineariswith disrupting property against preformed biofilm ofStaphylococcus aureus

et al. 2017

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Fast and effective photodynamic inactivation of 4-day-old biofilm of methicillin-resistant Staphylococcus aureus using methylene blue-conjugated gold nanoparticles

Cited by 50 publications

References 30 publications

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Radiofrequency electric field hyperthermia with gold nanostructures: role of particle shape and surface chemistry

Identification of α-tocopherol as a bioactive component ofDicranopteris lineariswith disrupting property against preformed biofilm ofStaphylococcus aureus

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