“…In an alternate analysis approach to the study of potential 2Ј-deoxycytidine deamination, 2Ј-deoxycytidinecontaining ODNs (10#5, 5#1, 5#6-5#14, Table 1) prepared by microwave-assisted deprotection were enzymatically hydrolyzed to mononucleosides by a combination of snake venom phosphodiesterase I (Crotalus adamanteus) (Williams et al, 1961;Bowman et al, 2001) and alkaline phosphatase (E. coli) (Reid and Wilson, 1971;McComb et al, 1979;Chandrasegaran et al, 1985;Marguet and Forterre, 2001;Gasan et al, 2002;Guerrero-Sanchez et al, 2006). Differential elution of the resultant mononucleosides by RP-HPLC was observed by UV-detection.…”