The emergence of the new generation video coding standard, Versatile Video Coding (VVC), has brought along novel features rendering the new standard more efficient and flexible than its predecessors. Aside from efficient compression of 8 k or higher camera-captured content, VVC also supports a wide range of applications, including computer-generated content, high dynamic range (HDR) content, multilayer and multi-view coding, video region extraction, as well as 360° video. One of the newly introduced coding tools in VVC, offering extraction and independent coding of rectangular sub-areas within a frame, is called Subpicture. In this work, we turn our attention to frame partitioning using Subpictures in VVC, and more particularly, a content-aware partitioning is considered. To achieve that, we make use of image segmentation algorithms and properly modify them to operate on a per Coding Tree Unit (CTU) basis in order to render them compliant with the standard’s restrictions. Additionally, since subpicture boundaries need to comply with slice boundaries, we propose two methods for properly partitioning a frame using tiles/slices aiming to avoid over-partitioning of a frame. The proposed algorithms are evaluated regarding both compression efficiency and image segmentation effectiveness. Our evaluation results indicate that the proposed partitioning schemes have a negligible impact on compression efficiency and video quality