Fast detection of drug interaction in Mycobacterium tuberculosis by a checkerboard resazurin method

Caleffi-Ferracioli, Katiany Rizzieri; Maltempe, Flaviane Granero; Siqueira, Vera Lúcia Dias; Cardoso, Rosilene Fressatti

doi:10.1016/j.tube.2013.09.001

Cited by 65 publications

(54 citation statements)

References 27 publications

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“…The MIC was defined as the lowest concentration that resulted in resazurin color change from blue to pink, which indicates its reduction by bacterial growth. Isolates with MIC ≤0.25 µg/ml [20], 0.5 µg/ml [21] and 2 µg/ml [22] were considered susceptible to INH, RIF and EMB, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The interactions between the drugs were tested by 3 independent assays in triplicate using the resazurin drug combination microtiter assay [22], a modified checkerboard method. The assay was performed in 96-well microplates (Kartell, Milan, Italy), in which the combinations were tested two-dimensionally (INH combined with CasIIIia, CasIIIEa, and CasIIgly; RIF with CasIIIia, CasIIIEa, and CasIIgly; EMB with CasIIIia, CasIIIEa, and CasIIgly).…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate possible synergistic effects, the fractional inhibitory concentration index (FICI) was calculated: FICI = (MIC A + B/MIC A) + (MIC B + A/MIC B), in which MIC A + B is the MIC of drug A combined with drug B, MIC B + A is the MIC of drug B combined with drug A, MIC A is the MIC of drug A tested alone, and MIC B is the MIC of drug B tested alone. The effects of the combinations were classified as synergistic (FICI ≤0.5), additive or indifferent (FICI >0.5-4.0), and antagonistic (FICI >4.0) [22]. …”

Section: Methodsmentioning

confidence: 99%

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Potential of Casiopeínas® Copper Complexes and Antituberculosis Drug Combination against Mycobacterium tuberculosis

Barbosa¹,

Caleffi-Ferracioli

Leite

et al. 2016

Chemotherapy

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New compounds with antituberculosis activity and their combination with classic drugs have been evaluated to determine possible interactions and antagonism. The aim of this study was to evaluate the in vitro activity of Casiopeínas® copper-based compounds (CasIIIia, CasIIIEa, and CasIIgly) alone and combined with isoniazid (INH), rifampicin, or ethambutol (EMB) against resistant and susceptible Mycobacterium tuberculosis. Seventeen clinical M. tuberculosis isolates (5 multi-drug resistant and 2 resistant to INH and/or EMB) were subjected to determination of the minimal inhibitory concentration (MIC) by the resazurin microtiter assay and combination assessment by the resazurin drug combination microtiter assay. The Casiopeínas® alone showed a remarkable effect against resistant isolates with MIC values from 0.78 to 12.50 μg/ml. Furthermore, a synergistic effect mainly with EMB is shown for both resistant and susceptible clinical isolates. Casiopeínas® are promising candidates for future investigation into the development of antituberculosis drugs, being one of the first examples of essential metal-based drugs used in this field.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Potential of Casiopeínas® Copper Complexes and Antituberculosis Drug Combination against Mycobacterium tuberculosis

Barbosa¹,

Caleffi-Ferracioli

Leite

et al. 2016

Chemotherapy

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“…Synergy investigations using a modified checkerboard MABA were adopted from Bhusal et al, [33] and Caleffi-Ferracioli et al, [34]. Culture, antimicrobials, media and 96-well microtiter plates were prepared as described (2.3) except for certain used, and synergy calculations were conducted using ∑FIC [33,34], where the definition of synergy was; synergy < 0.5, indifference was 0.5-4 and antagonism was > 4.…”

Section: Synergy Activity Investigation Of Cms With Inh and Rifmentioning

confidence: 99%

“…Culture, antimicrobials, media and 96-well microtiter plates were prepared as described (2.3) except for certain used, and synergy calculations were conducted using ∑FIC [33,34], where the definition of synergy was; synergy < 0.5, indifference was 0.5-4 and antagonism was > 4. Synergy investigations were conducted using three independent experiments.…”

Section: Synergy Activity Investigation Of Cms With Inh and Rifmentioning

confidence: 99%

The antimicrobial effect of colistin methanesulfonate on Mycobacterium tuberculosis in vitro

Breda¹,

Buys²,

Apostolides³

et al. 2015

Tuberculosis

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Please cite this article in press as: van Breda SV, et al., The antimicrobial effect of colistin methanesulfonate on Mycobacterium tuberculosis in vitro, Tuberculosis (2015), http://dx.doi.org/10.1016/j.tube.2015.05.005 SUMMARYPolymyxins have previously been described to have activity against M. tuberculosis (MTB), but further research was abandoned due to systemic toxicity concerns to achieve the required MIC. Colistin methanesulfonate (CMS), a polymyxin, is well tolerated when inhaled directly into the lungs, resulting in high local concentrations.We report here for the first time, MIC and MBC data for CMS determined by the microtiter Alamar Blue assay (MABA). We also determined how the MIC would be

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Green Phytosynthesis of Silver Nanoparticles Using Echinochloa stagnina Extract with Reference to Their Antibacterial, Cytotoxic, and Larvicidal Activities

2021

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Fast detection of drug interaction in Mycobacterium tuberculosis by a checkerboard resazurin method

Cited by 65 publications

References 27 publications

Potential of Casiopeínas® Copper Complexes and Antituberculosis Drug Combination against <b><i>Mycobacterium tuberculosis</i></b>

Potential of Casiopeínas® Copper Complexes and Antituberculosis Drug Combination against <b><i>Mycobacterium tuberculosis</i></b>

The antimicrobial effect of colistin methanesulfonate on Mycobacterium tuberculosis in vitro

Green Phytosynthesis of Silver Nanoparticles Using Echinochloa stagnina Extract with Reference to Their Antibacterial, Cytotoxic, and Larvicidal Activities

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