Green Phytosynthesis of Silver Nanoparticles Using Echinochloa stagnina Extract with Reference to Their Antibacterial, Cytotoxic, and Larvicidal Activities

Shehabeldine, Amr M.; Elbahnasawy, Mostafa A.; Hasaballah, Ahmed I.

doi:10.1007/s12668-021-00846-1

Cited by 33 publications

(30 citation statements)

References 53 publications

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“…Using a Spectrum Two IR Spectrometer (PerkinElmer Inc., Shelton, WA, USA) and these techniques, the total internal reflectance/Fourier-transform Infrared (ATR-FTIR) spectra was used to semi-quantitatively measure the observable IR spectrum of the Chi/Ag-NPs by evaluating the transmittance over a spectral region of 4000 to 400 cm −1 . To achieve a suitable signal quality, all spectra were collected at a 4 cm −1 resolution by collecting 32 scans [ 34 ]. A Diano X-ray diffractometer (Philips) with a CuK radiation source (λ = 0.15418 nm) activated at 45 kV, as well as a generator (PW, 1930) and a goniometer (PW, 1820), was used to study the XRD pattern of the produced Chi/Ag-NPs.…”

Section: Methodsmentioning

confidence: 99%

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Shehabeldine

Salem

Ali

et al. 2022

JoF

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The purpose of this study is to create chitosan-stabilized silver nanoparticles (Chi/Ag-NPs) and determine whether they were cytotoxic and also to determine their characteristic antibacterial, antibiofilm, and wound healing activities. Recently, the development of an efficient and environmentally friendly method for synthesizing metal nanoparticles based on polysaccharides has attracted a lot of interest in the field of nanotechnology. Colloidal Chi/Ag-NPs are prepared by chemical reduction of silver ions in the presence of Chi, giving Chi/Ag-NPs. Physiochemical properties are determined by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) analyses. TEM pictures indicate that the generated Chi/Ag-NPs are nearly spherical in shape with a thin chitosan covering around the Ag core and had sizes in the range of 9–65 nm. In vitro antibacterial activity was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa by a resazurin-mediated microtiter plate assay. The highest activity was observed with the lowest concentration of Chi/Ag-NPs, which was 12.5 µg/mL for both bacterial strains. Additionally, Chi/Ag-NPs showed promising antifungal features against Candida albicans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger, where inhibition zones were 22, 29, 20, and 17 mm, respectively. Likewise, Chi/Ag-NPs revealed potential antioxidant activity is 92, 90, and 75% at concentrations of 4000, 2000, and 1000 µg/mL, where the IC50 of Chi/Ag-NPs was 261 µg/mL. Wound healing results illustrated that fibroblasts advanced toward the opening to close the scratch wound by roughly 50.5% after a 24-h exposure to Chi/Ag-NPs, greatly accelerating the wound healing process. In conclusion, a nanocomposite based on AgNPs and chitosan was successfully prepared and exhibited antibacterial, antibiofilm, antifungal, antioxidant, and wound healing activities that can be used in the medical field.

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Section: Methodsmentioning

confidence: 99%

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Shehabeldine

Salem

Ali

et al. 2022

JoF

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“…Plant products/by-products have become a promising alternative to synthetic chemical insecticides [ 4 , 5 , 6 ]. Plants contain phytochemicals that belong to different classes, such as steroids, alkaloids, terpenes, and phenolics.…”

Section: Introductionmentioning

confidence: 99%

Mosquitocidal Activity of the Methanolic Extract of Annickia chlorantha and Its Isolated Compounds against Culex pipiens, and Their Impact on the Non-Target Organism Zebrafish, Danio rerio

Selim

Rahman

Mahran

et al. 2022

Insects

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In this study, the crude extract and its isolated compounds from the stem bark of Annickiachlorantha were tested for their larvicidal, developmental, and repellent activity against the mosquito vector, Culex pipiens, besides their toxicity to the non-target aquatic organism, the zebrafish (Danio rerio). The acute larvicidal activity of isolated compounds; namely, palmatine, jatrorrhizine, columbamine, β-sitosterol, and Annickiachlorantha methanolic extract (AC), was observed. Developmentally, the larval duration was significantly prolonged when palmatine and β-sitosterol were applied, whereas the pupal duration was significantly prolonged for almost all treatments except palmatine and jatrorrhizine, where it shortened from those in the control. Acetylcholinesterase (AChE) enzyme showed different activity patterns, where it significantly increased in columbamine and β-sitosterol, and decreased in (AC), palmatine, and jatrorrhizine treatments, whereas glutathione S-transferase (GST) enzyme was significantly increased when AC methanolic extract/isolated compounds were applied, compared to the control. The adult emergence percentages were significantly decreased in all treatments, whereas tested compounds revealed non-significant (p > 0.05) changes in the sex ratio percentages, with a slight female-to-male preference presented in the AC-treated group. Additionally, the tested materials revealed repellence action; interestingly, palmatine and jatrorrhizine recorded higher levels of protection, followed by AC, columbamine, and β-sitosterol for 7 consecutive hours compared to the negative and positive control groups. The non-target assay confirms that the tested materials have very low toxic activity compared to the reported toxicity against mosquito larvae. A docking simulation was employed to better understand the interaction of the isolated compounds with the enzymes, AChE and GST. Additionally, DFT calculations revealed that the reported larvicidal activity may be due to the differing electron distributions among tested compounds. Overall, this study highlights the potential of A. chlorantha extract and its isolated compounds as effective mosquitocidal agents with a very low toxic effect on non-target organisms.

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“…Several plant extract mediated AgNPs against multi-drug resistant bacterial pathogens have been explored recently due to the widespread antibiotic resistance around the world. 48–50…”

Section: Resultsmentioning

confidence: 99%

“…Several plant extract mediated AgNPs against multi-drug resistant bacterial pathogens have been explored recently due to the widespread antibiotic resistance around the world. [48][49][50] It has been reported that AgNPs can attach to the bacterial cell wall, causing physical changes in the membrane and leading to cell leakage and bacterial cell death. The existing difference in the cell wall thickness due to the layer of peptidoglycan between Gram-positive and Gram-negative bacteria is responsible for the difference in antibacterial activity.…”

Section: Njc Papermentioning

confidence: 99%

Synergistic actions of phytonutrient capped nanosilver as a novel broad-spectrum antimicrobial agent: unveiling the antibacterial effectiveness and bactericidal mechanism

Moorthy

Chang

et al. 2022

New J. Chem.

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Green Phytosynthesis of Silver Nanoparticles Using Echinochloa stagnina Extract with Reference to Their Antibacterial, Cytotoxic, and Larvicidal Activities

Cited by 33 publications

References 53 publications

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Multifunctional Silver Nanoparticles Based on Chitosan: Antibacterial, Antibiofilm, Antifungal, Antioxidant, and Wound-Healing Activities

Mosquitocidal Activity of the Methanolic Extract of Annickia chlorantha and Its Isolated Compounds against Culex pipiens, and Their Impact on the Non-Target Organism Zebrafish, Danio rerio

Synergistic actions of phytonutrient capped nanosilver as a novel broad-spectrum antimicrobial agent: unveiling the antibacterial effectiveness and bactericidal mechanism

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