Fast detection of Salmonella Infantis with carbon nanotube field effect transistors

Villamizar, Raquel A.; Maroto, Alicia; Rius, F. Xavier; Inza, Isabel; Figueras, María José

doi:10.1016/j.bios.2008.03.046

Cited by 172 publications

(89 citation statements)

References 23 publications

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“…As bacterial concentrations increased, the resistance differences also increased up to the bacterial concentration of 8 log CFU/mL. This is a typical characteristic of the functionalized CNT-based biosensor because the binding of the antibody with bacteria results in an increase in the resistance of the biosensor [21,26]. The linear regression of the resistance differences with selected concentrations of bacterial culture is presented in Figure 6.…”

Section: Limit Of Detection Of Swcnt-basedmentioning

confidence: 88%

“…When the biosensor was applied with S. aureus cell culture, the SEM image revealed that the S. aureus was captured onto the surface of the SWCNT-based biosensor ( Figure 5(b)). Villamizar et al [26] also reported that SEM confirmed the number of Salmonella cells attached to the CNTs was proportional to the concentration of Salmonella in solution.…”

Section: Sem Images Of S Aureus Cells Captured On the Swcntbasedmentioning

confidence: 94%

“…Other researchers reported similar results with functionalization of antibodies onto the CNTs by measuring electric current changes. Villamizar et al [26] reported that the functionalization steps of anti-Salmonella antibodies decreased the electrical current of a typical CNT field effect transistor. The electrical current of the device decreased after the absorption of anti-Salmonella due to the charge transfer process in which the antibodies provide electrons to the CNTs [27].…”

Section: Immobilization Of Antibody On Swcnt-based Biosensormentioning

confidence: 99%

“…Therefore, the limit of detection (LOD) of SWCNT-based biosensor was determined to be the concentration of 10 4 CFU/mL. Villamizar et al [26] reported that the biosensor response was a linear function of the logarithm of the bacterial concentrations between 3 and 7 log CFU/mL ( 2 = 0.93). Pyun et al [28] also reported that the limit of detection was determined to be 3.0 × 10 5 CFU/mL, when a flexural plate wave transducer was used for the detection of E. coli.…”

Section: Limit Of Detection Of Swcnt-basedmentioning

confidence: 99%

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Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Choi

Lee

Park

et al. 2017

Journal of Food Quality

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The goal of this research is to develop a single-walled carbon nanotube-(SWCNT-) based biosensor to detect Staphylococcus aureus. The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA. The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE). The resistance difference (Δ ) was calculated using a potentiostat. The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM). The optimum concentration of SWCNTs on the platform was determined to be 0.1 mg/mL. The binding of pAbs with S. aureus resulted in a significant increase in resistance value of the biosensor ( < 0.05). The SEM images confirmed the specific binding of S. aureus on the biosensor. The SWCNT-based biosensor was able to detect S. aureus with a limit of detection (LOD) of 4 log CFU/mL.

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Section: Limit Of Detection Of Swcnt-basedmentioning

confidence: 88%

Section: Sem Images Of S Aureus Cells Captured On the Swcntbasedmentioning

confidence: 94%

Section: Immobilization Of Antibody On Swcnt-based Biosensormentioning

confidence: 99%

Section: Limit Of Detection Of Swcnt-basedmentioning

confidence: 99%

See 2 more Smart Citations

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Choi

Lee

Park

et al. 2017

Journal of Food Quality

View full text Add to dashboard Cite

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“…This is probably due to the small number of examples studied and the small sensing surfaces of devices. Villamizar et al 64 reported a method of using anti-Salmonella antibodies to functionalize SWNT network-based FETs, which were subsequently protected by Tween 20 to prevent nonspecific binding (NSB) of other proteins, for the selective determination of S. infantis at low concentration (at least 100 c.f.u. ml À1 ).…”

Section: Swntàfunctionalization To Increase Sensitivitymentioning

confidence: 99%

Carbon nanomaterials field-effect-transistor-based biosensors

2012

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Carbon nanomaterials field-effect transistor (FET)-based electrical biosensors provide significant advantages over the current gold standards, holding great potential for realizing direct, label-free, real-time electrical detection of biomolecules in a multiplexed manner with ultrahigh sensitivity and excellent selectivity. The feasibility of integrating them with current complementary metal oxide semiconductor platform and a fluid handling module using standard microfabrication technology opens up new opportunities for the development of low-cost, low-noise, portable electrical biosensors for use in practical future devices. In this article, we review recent progress in the rapidly developing area of biomolecular interaction detection using FET-based biosensors based on the carbon nanomaterials single-walled carbon nanotubes (SWNTs) and graphene. Detection scenarios include DNA-DNA hybridization, DNA-protein interaction, protein function and cellular activity. In particular, we will highlight an amazing property of SWNT-or graphene-FETs in biosensing: their ability to detect biomolecules at the single-molecule level or at the single-cell level. This is due to the size comparability and the surface compatibility of the carbon nanomaterials with biological molecules. We also summarize some current challenges the scientific community is facing, including device-to-device heterogeneity and the lack of system integration for uniform device array mass production. Keywords: biosensor; carbon nanotube; field-effect transistor; graphene A biosensor is an analytical device that incorporates a biological recognition element in direct spatial contact with a transduction element. This integration ensures the rapid and convenient conversion of the biological events to detectable signals. 1 With the discovery of rich nanomaterials and the development of exquisite nanofabrication tools, such as electron beam lithography, focused ion beam and nanoimprint lithography, new avenues have been opened up in the field of biosensors in the last two decades. 2,3 In particular, researchers around the world have been tailor-making a multitude of nanomaterials-based electrical biosensors and developing new strategies to apply them in ultrasensitive biosensing. Examples of such nanomaterials include carbon nanotubes, 4-13 nanowires, 11,14-21 nanoparticles, 6,22-25 nanopores, 26,27 nanoclusters 28 and graphene. 5,[29][30][31][32] Compared with conventional optical, biochemical and biophysical methods, nanomaterial-based electronic biosensing offers significant advantages, such as high sensitivity and new sensing mechanisms, high spatial resolution for localized detection, facile integration with standard wafer-scale semiconductor processing and label-free, real-time detection in a nondestructive manner.Among diverse electrical biosensing architectures, devices based on field-effect transistors (FETs) have attracted great attention because they are an ideal biosensor that can directly translate the interactions between target biological mole...

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Prevention and Control of Biofilms in the Food Industry and Bio‐Nanotechnology Approaches

Birkenhauer

Neethirajan

2015

Biofilms in the Food Environment

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Fast detection of Salmonella Infantis with carbon nanotube field effect transistors

Cited by 172 publications

References 23 publications

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Carbon nanomaterials field-effect-transistor-based biosensors

Prevention and Control of Biofilms in the Food Industry and Bio‐Nanotechnology Approaches

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