Fast detection of Southern tomato virus by one-step transcription loop-mediated isothermal amplification (RT-LAMP)

Elvira-González, Laura; Puchades, Alice; Carpino, C.; Alfaro-Fernández, A.; Ambrosio, María Isabel Font San; Rubio, Luís; Galipienso, Luis

doi:10.1016/j.jviromet.2016.12.004

Cited by 30 publications

(8 citation statements)

References 16 publications

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“…Others have reported that nucleic acid extraction may not be necessary with some pathogens if the temperature is high enough in the reaction to allow the virion to become permeable by primers and enzymes providing access to viral RNA. The enzymes utilized in LAMP are more resistant to inhibitory components in clinical specimens [ 44 , 53 – 56 ].…”

Section: Discussionmentioning

confidence: 99%

Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

et al. 2017

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Zika virus (ZIKV) has emerged as a major global public health concern in the last two years due to its link as a causative agent of human birth defects. Its rapid expansion into the Western Hemisphere as well as the ability to be transmitted from mother to fetus, through sexual transmission and possibly through blood transfusions has increased the need for a rapid and expansive public health response to this unprecedented epidemic. A non-invasive and rapid ZIKV diagnostic screening assay that can be performed in a clinical setting throughout pregnancy is vital for prenatal care of women living in areas of the world where exposure to the virus is possible. To meet this need we have developed a sensitive and specific reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay to detect ZIKV RNA in urine and serum with a simple visual detection. RT-LAMP results were shown to have a limit of detection 10-fold higher than qRT-PCR. As little as 1.2 RNA copies/μl was detected by RT-LAMP from a panel of 178 diagnostic specimens. The assay was shown to be highly specific for ZIKV RNA when tested with diagnostic specimens positive for dengue virus (DENV) and chikungunya virus (CHIKV). The assay described here illustrates the potential for a fast, reliable, sensitive and specific assay for the detection of ZIKV from urine or serum that can be performed in a clinical or field setting with minimal equipment and technological expertise.

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Section: Discussionmentioning

confidence: 99%

Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

et al. 2017

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“…Moreover, it also has additional advantage of direct observation of the results or by using some intercalating dyes. Various plant viruses like Barley yellow dwarf viruses [107], Cucumber mosaic virus [108], Potato leafroll virus [109], Southern tomato virus (STV) [110], Tomato brown rugose fruit virus (ToBRFV) [111], and Citrus leaf blotch virus in kiwifruit [112] have been detected using LAMP PCR and RT-PCR assays. Indian citrus ringspot virus (ICRSV) was analyzed using a novel, one-step RT-LAMP method by designing and testing four different primers that targets coat protein gene of ICRSV [113].…”

Section: Pcr-based Methodsmentioning

confidence: 99%

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Mehetre

Leo

Singh³

et al. 2021

Viruses

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Plant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective treatment and prevention of viral diseases, there is great demand for new techniques that can provide accurate identification on the causative agents. With the advancements in biochemical and molecular biology techniques, several diagnostic methods with improved sensitivity and specificity for the detection of prevalent and/or unknown plant viruses are being continuously developed. Currently, serological and nucleic acid methods are the most widely used for plant viral diagnosis. Nucleic acid-based techniques that amplify target DNA/RNA have been evolved with many variants. However, there is growing interest in developing techniques that can be based in real-time and thus facilitate in-field diagnosis. Next-generation sequencing (NGS)-based innovative methods have shown great potential to detect multiple viruses simultaneously; however, such techniques are in the preliminary stages in plant viral disease diagnostics. This review discusses the recent progress in the use of NGS-based techniques for the detection, diagnosis, and identification of plant viral diseases. New portable devices and technologies that could provide real-time analyses in a relatively short period of time are prime important for in-field diagnostics. Current development and application of such tools and techniques along with their potential limitations in plant virology are likewise discussed in detail.

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“…Thus, several protocols in plant pathology were developed, where crude plant saps were used as template. This implementation accelerated the time for the diagnosis, avoiding any previous step for samples preparation and DNA or RNA purification [26,[34][35][36][37][38][39]. In that way, diagnosis time was speeded up for 2-3 h, which was necessary to complete the extraction of DNA/RNA of target organism.…”

Section: Lamp Accelerationmentioning

confidence: 99%

Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology

Panno

Matić

Tiberini³

et al. 2020

Plants

156

111

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In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique.

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Fast detection of Southern tomato virus by one-step transcription loop-mediated isothermal amplification (RT-LAMP)

Cited by 30 publications

References 16 publications

Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology

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