Fast determination of paraquat in plasma and urine samples by solid-phase microextraction and gas chromatography–mass spectrometry

Gao, Lina; Liu, Junting; Wang, Chun-Yuan; Liu, Guojie; Niu, Xiaodong; Shu, C.-M.; Zhu, Juan

doi:10.1016/j.jchromb.2013.10.028

Cited by 52 publications

(24 citation statements)

References 26 publications

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“…Percentage of eosinophils PE F 5 Percentage of basophilic granulocyte PBG F 6 Percentage of monocyte PMC F 7 Percentage of leukomonocyte PLC F 8 Absolute value of eosinophils AVE F 9 Absolute value of neutrophilic granulocyte AVNG F 10 Absolute value of monocyte AVM F 11 Absolute value of leukomonocyte AVLC F 12 Absolute value of basophilic granulocyte AVBG F 13 Red blood cell RBC F 14 Haemoglobin HB F 15 Haematocrit HCT F 16 Mean corpuscular volume MCV F 17…”

Section: Preliminary Descriptionmentioning

confidence: 99%

“…Studies have shown that the mortality rate of PQ poisoning is highly correlated with ingested PQ amount or PQ concentrations in plasma and urine (within 4-24 hr elapsed from ingestion) [4,5]. Hsu et al [6] found that early haemoperfusion (HP) after PQ exposure may reduce the mortality specifically and that HP <4.0 or <5.0 hr can reduce the relative risk of mortality by approximately 81% or 51%, respectively.Although PQ concentration has proven effective in terms of PQ poisoning diagnosis and prognosis, it is seldom applied in clinics [7,8] because it must be determined by HPLC or LC-MS, which requires highly accurate equipment [9,10].Although sodium dithionite, sodium hydroxide and spectrophotometry can be used to determine PQ concentration, they are simple qualitative assays and cannot provide the accurate level of PQ concentration. Consequently, PQ concentration is generally not available as a routine test option in hospitals.…”

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An Effective Machine Learning Approach for Prognosis of Paraquat Poisoning Patients Using Blood Routine Indexes

Chen

et al. 2016

Basic Clin Pharma Tox

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The early identification of toxic paraquat (PQ) poisoning in patients is critical to ensure timely and accurate prognosis. Although plasma PQ concentration has been reported as a clinical indicator of PQ poisoning, it is not commonly applied in practice due to the inconvenient necessary instruments and operation. In this study, we explored the use of blood routine indexes to identify the degree of PQ toxicity and/or diagnose PQ poisoning in patients via machine learning approach. Specifically, we developed a method based on support vector machine combined with the feature selection technique to accurately predict PQ poisoning risk status, then tested the method on 79 (42 male and 37 female; 41 living and 38 deceased) patients. The detection method was rigorously evaluated against a real-world data set to determine its accuracy, sensitivity and specificity. Feature selection was also applied to identify the factors correlated with risk status, and the results showed that there are significant differences in blood routine indexes between dead and living PQ-poisoned individuals (p-value < 0.01). Feature selection also showed that the most important correlated indexes are white blood cell and neutrophils. In conclusion, the toxicity or prognosis of PQ poisoning can be preliminarily ascertained by blood routine testing without PQ concentration data, representing an additional tool and innovative approach to assess the prognosis of PQ poisoning.of the most commonly used herbicides in the world, is considerably more toxic to human beings than other herbicidal agents. Acute ingestion of 7-8 mL PQ can cause severe liver, lung and kidney issues and even heart failure leading to death [1]; the mortality of PQ poisoning is 60-70% [2]. Many people have been poisoned by PQ throughout many years and across the world, although the problem is especially serious in developing countries. Early prognosis of PQ poisoning is significant for both patients and doctors [3]. Studies have shown that the mortality rate of PQ poisoning is highly correlated with ingested PQ amount or PQ concentrations in plasma and urine (within 4-24 hr elapsed from ingestion) [4,5]. Hsu et al. [6] found that early haemoperfusion (HP) after PQ exposure may reduce the mortality specifically and that HP <4.0 or <5.0 hr can reduce the relative risk of mortality by approximately 81% or 51%, respectively.Although PQ concentration has proven effective in terms of PQ poisoning diagnosis and prognosis, it is seldom applied in clinics [7,8] because it must be determined by HPLC or LC-MS, which requires highly accurate equipment [9,10].Although sodium dithionite, sodium hydroxide and spectrophotometry can be used to determine PQ concentration, they are simple qualitative assays and cannot provide the accurate level of PQ concentration. Consequently, PQ concentration is generally not available as a routine test option in hospitals.The blood routine test (BRT), also named complete blood count, which includes many indexes such as white blood cell (WBC), red blo...

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Section: Preliminary Descriptionmentioning

confidence: 99%

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confidence: 99%

An Effective Machine Learning Approach for Prognosis of Paraquat Poisoning Patients Using Blood Routine Indexes

Chen

et al. 2016

Basic Clin Pharma Tox

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“…SPE is also employed for the clean-up of hair extracts. Recently, Duca et al [83] evaluated several solid-phase extraction cartridges for the purification of 67 metabolites and pesticides of different chemical classes extracted from hair. They concluded that a dual-layer cartridge of graphitized carbon black (GCB)/primary and secondary amines (PSA) was clearly the best suited to non-polar compounds such as organochlorines, pyrethroids and organophosphates,).…”

Section: Extraction and Clean-up Strategiesmentioning

confidence: 99%

Analytical methods for human biomonitoring of pesticides. A review

Yusà

Millet

Coscollà

et al. 2015

Analytica Chimica Acta

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“…Derivatization is essential for GC analysis of less volatile components and the volatile 6 derivatives are usually prepared before SPME [22,23]. Hollow fibers (HF), with crossed fibroid microporous structure, has been used as carriers of extraction solvent and sorbent for its high specific surface area and large molecules exclusion effects.…”

Section: Introductionmentioning

confidence: 99%

Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry

Sun

Chen

et al. 2015

Journal of Chromatography B

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Fast determination of paraquat in plasma and urine samples by solid-phase microextraction and gas chromatography–mass spectrometry

Cited by 52 publications

References 26 publications

An Effective Machine Learning Approach for Prognosis of Paraquat Poisoning Patients Using Blood Routine Indexes

An Effective Machine Learning Approach for Prognosis of Paraquat Poisoning Patients Using Blood Routine Indexes

Analytical methods for human biomonitoring of pesticides. A review

Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry

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