Fast nanomechanical spectroscopy of soft matter

Herruzo, Elena T.; Perrino, Alma P.; García, Ricardo García

doi:10.1038/ncomms4126

Cited by 217 publications

(243 citation statements)

References 52 publications

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“…In terms of the observable / 6 this implies that / 6 > 90 throughout (Fig. 2(c)) in accordance with the prediction of (12). According to the simulations here, these results should be standard in bimodal AFM in the attractive regime where F AV < 0.…”

supporting

confidence: 89%

“…Multifrequency atomic force microscopy (AFM) is an emerging 1,2 branch of AFM where two or more frequencies 3,4 are externally excited in order to map material composition, [5][6][7] enhance resolution 8 and sensitivity, [9][10][11] and quantify material properties [12][13][14] with gentle forces. The theory that controls the response of the cantilever while simultaneously exciting several frequencies and modes however is still emerging 12,15,16 and might result complex. 17,18 Here, possible mechanisms responsible for multiple regimes of operation in multifrequency AFM are discussed from the point of view of energy transfer in the presence of conservative and dissipative interactions.…”

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confidence: 99%

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Phase contrast and operation regimes in multifrequency atomic force microscopy

Santos

2014

Applied Physics Letters

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In amplitude modulation atomic force microscopy the attractive and the repulsive force regimes induce phase shifts above and below 90°, respectively. In the more recent multifrequency approach, however, multiple operation regimes have been reported and the theory should be revisited. Here, a theory of phase contrast in multifrequency atomic force microscopy is developed and discussed in terms of energy transfer between modes, energy dissipation and the kinetic energy and energy transfer associated with externally driven harmonics. The single frequency virial that controls the phase shift might undergo transitions in sign while the average force (modal virial) remains positive (negative). © 2014 AIP Publishing LLC.Peer ReviewedPostprint (published version

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supporting

confidence: 89%

mentioning

confidence: 99%

Phase contrast and operation regimes in multifrequency atomic force microscopy

Santos

2014

Applied Physics Letters

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“…Overcoming these challenges will require the efforts of researchers from different disciplines (eg, biology, engineering, physics, automation). Nonetheless, it is gratifying to see that in recent years important progress has been made to enhance the functions of AFM, such as high-speed imaging (to improve the scan speed) [41] , multiparametric mode (to simultaneously obtain multiple parameters) [23] , high-speed force spectroscopy (to improve the speed of obtaining force curves) [103] , nanofluidics AFM (to allow the injection of chemical molecules or drugs into a single cell) [120] , tomographic contact resonance AFM (to generate cross-sectional stiff images of samples) [121] and fast nanomechanical spectroscopy (to improve the speed of mapping the mechanical properties) [122] . Moreover, AFM can also be combined with other techniques to obtain complementary information, and this will be an important aspect for future AFM studies.…”

Section: Challenge and Outlookmentioning

confidence: 99%

Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy

Liu

et al. 2015

Acta Pharmacol Sin

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Knowledge of the nanoscale changes that take place in individual cells in response to a drug is useful for understanding the drug action. However, due to the lack of adequate techniques, such knowledge was scarce until the advent of atomic force microscopy (AFM), which is a multifunctional tool for investigating cellular behavior with nanometer resolution under near-physiological conditions. In the past decade, researchers have applied AFM to monitor the morphological and mechanical dynamics of individual cells following drug stimulation, yielding considerable novel insight into how the drug molecules affect an individual cell at the nanoscale. In this article we summarize the representative applications of AFM in characterization of drug actions on cell membrane, including topographic imaging, elasticity measurements, molecular interaction quantification, native membrane protein imaging and manipulation, etc. The challenges that are hampering the further development of AFM for studies of cellular activities are aslo discussed.

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“…20 In order to obtain sample sizes in the order of 10−30 data points, this would imply acquiring at least 2000−9000 data points per experiment. Since force measurements require at least fractions of a second, 11,13,14,21,22 the associated time-cost would be considerable for standard experimentation.…”

Section: Introductionmentioning

confidence: 99%

Establishing Nanoscale Heterogeneity with Nanoscale Force Measurements

et al. 2015

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Establishing the presence or absence of nanoscale compositional heterogeneity with nanoscale resolution is becoming instrumental for the development of many fields of science. Force versus distance measurements and parameters directly or indirectly derived from these profiles can be potentially employed for this purpose with sophisticated instruments such as the atomic force microscope (AFM). On the other hand, standards are necessary to reproducibly and conclusively support hypothesis from experimental data and these standards are still emerging. Here, we define a set of standards for providing data originating from atomic force measurements to be employed to compare between sample properties, parameters, or, more generally, compositional heterogeneity. We show that reporting the mean and standard deviation only might lead to inconsistent conclusions. The fundamental principle behind our investigation deals with the very definition of reproducibility and repeatability in terms of accuracy and precision, and we establish general criteria to ensure that these hold without the need of restricting assumptions.

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Fast nanomechanical spectroscopy of soft matter

Cited by 217 publications

References 52 publications

Phase contrast and operation regimes in multifrequency atomic force microscopy

Phase contrast and operation regimes in multifrequency atomic force microscopy

Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy

Establishing Nanoscale Heterogeneity with Nanoscale Force Measurements

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