Fast profiling of anthocyanins in wine by desorption nano-electrospray ionization mass spectrometry

Hartmanová, Lucie; Ranc, Václav; Papoušková, Barbora; Bednář, Petr; Havlı́ček, Vladimı́r; Lemr, Karel

doi:10.1016/j.chroma.2010.03.018

Cited by 42 publications

(20 citation statements)

References 36 publications

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“…The same technique was also used in other studies [14][15][16] for the determination of anthocyanins in wines, grape extracts, and red and black currant extracts. HPLC-DAD-MS has been widely used for the analysis of anthocyanins in various samples, such as purple corn cob [17][18][19][20][21][22], while the LC-nano-DESI-MS has been used in red wine analysis [23]. Last but not least, the use of LC-MS/MS has been applied in order to analyze wine grape skins [24], berry skins [22,25], human saliva [26], elderberry extracts [27] and red wine vinegar [28].…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, the separation technique which is commonly applied is liquid chromatography using mainly C18 reversed-phase columns. This technique combined with UV-Vis (PDA (Photodiode array)) and/or mass spectrometry detectors has been used for the determination of anthocyanin content in various matrices [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

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A Sensitive LC-MS Method for Anthocyanins and Comparison of Byproducts and Equivalent Wine Content

et al. 2016

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Abstract:Anthocyanins are a group of phenolic compounds with great importance, not only because they play a crucial role in a wine's quality, but also due to the fact that they can have beneficial effects on human health. In this work, a method was developed for the detection and identification of these compounds in solid wastes of the wine-making industry (red grape skins and pomace), using liquid-liquid extraction (LLE) prior to the liquid chromatography-mass spectrometry technique (LC-MS). The complete process was investigated and optimized, starting from the extraction conditions (extraction solution selection, dried matter-to-solvent volume ratio, water bath extraction duration, and necessary consecutive extraction rounds) and continuing to the mobile phase selection. The extraction solution chosen was a methanol/phosphoric acid solution (95/5, v/v), while three rounds of consecutive extraction were necessary in order to extract the maximum amount of anthocyanins from the byproducts. During the LC-MS analysis, acetonitrile was selected as the organic solvent since, compared with methanol, not only did it exhibit increased elution strength, but it also produced significantly narrower peaks. To enable accurate identification of the analytes and optimization of the developed method, kuromanin chloride and myrtillin chloride were used as standards. Furthermore, the wine variety (Syrah) from which the specific byproducts were produced was analyzed for its anthocyanin content, leading to interesting conclusions about which anthocyanins are transferred from grapes to wine during the vinification procedure, and to what extent. The results of this study showed that the total concentration of anthocyanins estimated in wine byproducts exceeded almost 12 times the equivalent concentration in Syrah wine, while the four categories of detected anthocyanins, simple glucosides, acetyl glucosides, cinnamoyl glucosides, and pyroanthocyanins, were present in different ratios among the two samples, ranging from 18.20 to 1, to 5.83 to 1. These results not only confirmed the potential value of these byproducts, but also indicated the complexity of the anthocyanins' transfer mechanism between a wine and its byproducts.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Sensitive LC-MS Method for Anthocyanins and Comparison of Byproducts and Equivalent Wine Content

et al. 2016

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“…A variety of anthocyanins could be detected in red wine samples from different vintages and cultivars, as well as in berries. However, some isobaric compounds remained undistinguished, demonstrating the need for high mass accuracy measurements to improve the applicability of DESI in anthocyanin analysis [91]. Analysis of less polar compounds is also feasible by DESI-MS.…”

Section: Desi-hrmsmentioning

confidence: 98%

“…By using this approach, they confirmed decreased concentrations of the lachrymatory factor 3 and higher concentrations of sulfenic acid condensates in a tearless onion variety. Anthocyanin profile evaluation, used for detection of illicit wine coloring, was also carried out by DESI-TOF MS [91]. A variety of anthocyanins could be detected in red wine samples from different vintages and cultivars, as well as in berries.…”

Section: Desi-hrmsmentioning

confidence: 99%

The role of direct high-resolution mass spectrometry in foodomics

et al. 2015

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Foodomics has been defined as a global discipline in which advanced analytical techniques and bioinformatics are combined to address different questions in food science and nutrition. There is a growing number of works on the development and application of non-targeted omics methods in foodomics, which reflects that this emerging discipline is already considered by the scientific community to be a valuable approach to assess food safety, quality, and traceability as well as for the study of the links between food and health. As a result, there is a clear need for more rapid, high-throughput MS approaches for developing and applying non-targeted studies. Nowadays, direct MS analysis is one of the main choices to achieve high throughput, generating a set of information from the largest possible number of samples in a fast and straightforward way. The use of high- and ultrahigh-resolution MS greatly improves the analytical performance and offers a good combination of selectivity and sensitivity. By using a range of methods for direct sample introduction/desorption/ionization, high-throughput and non-target analysis of a variety of samples can be obtained in a few seconds by HRMS analysis. In this review, a general overview is presented of the main characteristics of direct HRMS-based approaches and their principal applications in foodomics.

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“…This allows the same colony to be sampled more than once using nanoDESI, gaining a time-lapse view of metabolites production and the roles of those metabolites in the colony. NanoDESI can be used to rapidly profile the metabolites from samples [97], assist in the characterization of specialized metabolites from microbial colonies [90] and to capture metabolites production involved in interspecies interactions [98]. However, nanoDESI has some drawbacks.…”

Section: Real-time Mass Spectrometrymentioning

confidence: 99%

Emerging mass spectrometry techniques for the direct analysis of microbial colonies

Fang

Dorrestein

2014

Current Opinion in Microbiology

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One of the emerging areas in microbiology is detecting specialized metabolites produced by microbial colonies and communities with mass spectrometry. In this review/perspective, we illustrate the emerging mass spectrometry methodologies that enable the interrogation of specialized metabolites directly from microbial colonies. Mass spectrometry techniques such as imaging mass spectrometry and real-time mass spectrometry allow two and three dimensional visualization of the distribution of metabolites, often with minimal sample pretreatment. The speed in which molecules are captured using these methods requires the development of new molecular visualization tools such as molecular networking. Together, these tools are beginning to provide unprecedented insight into the chemical world that microbes experience.

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Fast profiling of anthocyanins in wine by desorption nano-electrospray ionization mass spectrometry

Cited by 42 publications

References 36 publications

A Sensitive LC-MS Method for Anthocyanins and Comparison of Byproducts and Equivalent Wine Content

A Sensitive LC-MS Method for Anthocyanins and Comparison of Byproducts and Equivalent Wine Content

The role of direct high-resolution mass spectrometry in foodomics

Emerging mass spectrometry techniques for the direct analysis of microbial colonies

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