Fast Response Fluorescent Probe with a Large Stokes Shift for Thiophenol Detection in Water Samples and Cell Imaging

You, Jinjie; Liu, Hua; Pan, Qiao-Fen; Sun, Ailing; Zhang, Zeming; Shi, Xizhi

doi:10.1007/s41664-022-00247-7

Cited by 10 publications

(6 citation statements)

References 30 publications

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“…To address this issue, a fluorescent probe-based assay has emerged as a promising alternative for sensing and imaging cholinesterases, due to its high sensitivity and selectivity, simple operation, fast response speed, and good stability. − Some excellent works about the detection of the AChE − and BChE − and the discovery of cholinesterase inhibitors − have been reported. However, designing specific probes for distinguishing between AChE and BChE is very challenging.…”

Section: Introductionmentioning

confidence: 99%

Development of Cholinesterase-Activatable Fluorescent Probes for Pesticide Residue Detection

Li,

Zhang,

et al. 2024

ACS Agric. Sci. Technol.

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The two main types of cholinesterases, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), share similar structures and functions and are commonly present in biological environments. The activity of cholinesterase is closely related to pesticide residues; it is crucial to develop enzyme-inhibition-based fluorescent probes for pesticide residue detection. However, the discovery of a fluorescent probe that selectively targets one of these is consistently a formidable challenge. In this study, two kinds of fluorescent probes, HBT-A and HBT-B, were developed with 2-(2-hydroxyphenyl) benzothiazole (HBT) as the fluorophore, which can selectively distinguish AChE and BChE, respectively. Our probes (HBT-A for AChE and HBT-B for BChE) exhibited high sensitivity and specificity toward their respective analytes in cells and zebrafish. Finally, we used HBT-B as an example and demonstrated that it can be an effective tool for visualizing pesticide residues in living organisms, highlighting the potential application of the developed probes in environmental monitoring and food safety.

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Section: Introductionmentioning

confidence: 99%

Development of Cholinesterase-Activatable Fluorescent Probes for Pesticide Residue Detection

Li,

Zhang,

et al. 2024

ACS Agric. Sci. Technol.

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“…Up to now, a large number of organic small molecules have been developed by decorating functional recognition groups onto various fluorophores and acted as fluorescent probes for this purpose. [11][12][13][14][15][16][17][18][19] In combination with fluorescent microscopy, small organic fluorescent molecules exhibit indispensable advantages for insight into biological events, especially in the aspects of non-destructive imaging and visualization monitoring. [20][21][22][23][24][25][26][27] However, the crucial factor in probe design is to discover chemical reactions with high specificity toward the target and reasonably tune the optical performances.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, discovering new chemical reactions that occurred under mild conditions and can be applied in living systems to monitor cellular events is the important breakthrough. Up to now, a large number of organic small molecules have been developed by decorating functional recognition groups onto various fluorophores and acted as fluorescent probes for this purpose [11–19] . In combination with fluorescent microscopy, small organic fluorescent molecules exhibit indispensable advantages for insight into biological events, especially in the aspects of non‐destructive imaging and visualization monitoring [20–27] .…”

Section: Introductionmentioning

confidence: 99%

Electron‐withdrawing inductive effects enhanced strategy for protein thiol sensing and blocking agent design

Zhang,

Liu,

Zhang

et al. 2024

Smart Molecules

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It is a great challenge to discover novel chemical reactions suitable for biological analysis in a living system. The development of novel protein thiol blocking agents is a crucial need for exploring protein thiol functions in protein refolding, signal transduction, and redox regulation. We are always keen on seeking novel chemical reactions applied to endogenous biological macromolecules or protein thiol sensing, blocking, and labeling. In the present work, we have successfully developed a novel agent to block protein thiol by enhanced electron‐withdrawing inductive effects. This sensing and blocking process was detailedly monitored by UV‐vis, fluorescent spectra, and SDS‐Page gel separation. The spectral studies demonstrated that the agent could react ultrafastly with thiol within seconds at μM level. Furthermore, fluorescent imaging in cells and in vivo was further used for the validation of its ability to sensing and blocking thiol, providing evidence of downregulated protein thiols in Parkinson's disease. The enhanced electron‐withdrawing inductive effect strategy in this work may provide a general guideline for designing protein thiol agent.

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“…Moreover, none of these methods can identify the specific type of DNA damage and fail to clarifying the molecular mechanism of the damage caused by pollutant exposure. Recently, a fluorescent probe has been rapidly developed, being a sensitive and efficient approach to reveal spatial and temporal molecular information in living cells upon environmental stimulation. − In addition, the association between the repair enzyme and the DNA damage has drawn increasing attention, and the species and concentrations of repair enzymes serve as important information to identify the types and degrees of DNA damage in cells . When DNA damage occurs in cells, a large number of repair enzymes are generated to oppose the damage, and the extent of damage is indicated by the expression level of the repair enzymes.…”

mentioning

confidence: 99%

Development of a Repair Enzyme Fluorescent Probe to Reveal the Intracellular DNA Damage Induced by Benzo[a]pyrene in Living Cells

et al. 2023

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Pollutant exposure causes a series of DNA damage in cells, resulting in the initiation and progression of diseases and even cancers. An investigation of the DNA damage induced by pollutants in living cells is significant to evaluate the cytotoxicity, genotoxicity, and carcinogenicity of environmental exposure, providing critical insight in the exploration of the etiologies of diseases. In this study, we develop a repair enzyme fluorescent probe to reveal the DNA damage caused by an environmental pollutant in living cells by single-cell fluorescent imaging of the most common base damage repair enzyme named human apurinic/apyrimidinic endonuclease 1 (APE1). The repair enzyme fluorescent probe is fabricated by conjugation of an APE1 high affinity DNA substrate on a ZnO2 nanoparticle surface to form a ZnO2@DNA nanoprobe. The ZnO2 nanoparticle serves as both a probe carrier and a cofactor supplier, releasing Zn2+ to activate APE1 generated by pollutant exposure. The AP-site in the DNA substrate of the fluorescent probe is cleaved by the activated APE1, releasing fluorophore and generating fluorescent signals to indicate the position and degree of APE1-related DNA base damage in living cells. Subsequently, the developed ZnO2@DNA fluorescent probe is applied to investigate the APE1-related DNA base damage induced by benzo[a]pyrene (BaP) in living human hepatocytes. Significant DNA base damage by BaP exposure is revealed, with a positive correlation of the damage degree with exposure time in 2–24 h and the concentration in 5–150 μM, respectively. The experimental results demonstrate that BaP has a significant effect on the AP-site damage, and the degree of DNA base damage is time-dependent and concentration-dependent.

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Fast Response Fluorescent Probe with a Large Stokes Shift for Thiophenol Detection in Water Samples and Cell Imaging

Cited by 10 publications

References 30 publications

Development of Cholinesterase-Activatable Fluorescent Probes for Pesticide Residue Detection

Development of Cholinesterase-Activatable Fluorescent Probes for Pesticide Residue Detection

Electron‐withdrawing inductive effects enhanced strategy for protein thiol sensing and blocking agent design

Development of a Repair Enzyme Fluorescent Probe to Reveal the Intracellular DNA Damage Induced by Benzo[a]pyrene in Living Cells

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