Fast scanning coaxial optoacoustic microscopy

Ma, Rui; Sontges, Sebastian; Shoham, Sharon; Ntziachristos, Vasilis; Razansky, Daniel

doi:10.1364/boe.3.001724

Cited by 69 publications

(58 citation statements)

References 15 publications

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“…The emitted photoacoustic waves are then detected to reconstruct the optical absorption properties of the biological tissue, which correlate with many important physiological parameters, including the total concentration, the oxygen saturation, and the oxygen metabolism of hemoglobin. Optical-resolution photoacoustic microscopy (OR-PAM) is a specific form of PAT that offers optical-diffraction limited transverse spatial resolution, which can be as fine as micrometers or even sub-micrometers [2][3][4][5]. OR-PAM uses a tightly focused laser beam for photoacoustic excitation and a focused single-element ultrasonic transducer to record depth-resolved 1D photoacoustic images (A-lines).…”

Section: Introductionmentioning

confidence: 99%

Multi-parametric quantitative microvascular imaging with optical-resolution photoacoustic microscopy in vivo

Yang¹,

Chen²,

Yao³

et al. 2014

Opt. Express

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Many diseases involve either the formation of new blood vessels (e.g., tumor angiogenesis) or the damage of existing ones (e.g., diabetic retinopathy) at the microcirculation level. Optical-resolution photoacoustic microscopy (OR-PAM), capable of imaging microvessels in 3D in vivo down to individual capillaries using endogenous contrast, has the potential to reveal microvascular information critical to the diagnosis and staging of microcirculation-related diseases. In this study, we have developed a dedicated microvascular quantification (MQ) algorithm for OR-PAM to automatically quantify multiple microvascular morphological parameters in parallel, including the vessel diameter distribution, the microvessel density, the vascular tortuosity, and the fractal dimension. The algorithm has been tested on in vivo OR-PAM images of a healthy mouse, demonstrating high accuracy for microvascular segmentation and quantification. The developed MQ algorithm for OR-PAM may greatly facilitate quantitative imaging of tumor angiogenesis and many other microcirculation related diseases in vivo. M. Arbeit, "VEGF is essential for hypoxia-inducible factor-mediated neovascularization but dispensable for endothelial sprouting," Proc. Natl. Acad. Sci. U. S. A. 108(32), 13264-13269 (2011). 9. J. Folkman, "Angiogenesis in cancer, vascular, rheumatoid and other disease," Nat. Med. 1(1), 27-30 (1995 1182-1186 (1971). 11. J. W. Baish and R. K. Jain, "Cancer, angiogenesis and fractals," Nat. Med. 4(9), 984 (1998). 12. R. K. Jain, "Normalizing tumor vasculature with anti-angiogenic therapy: A new paradigm for combination therapy," Nat. Med. 7(9), 987-989 (2001)

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Section: Introductionmentioning

confidence: 99%

Multi-parametric quantitative microvascular imaging with optical-resolution photoacoustic microscopy in vivo

Yang¹,

Chen²,

Yao³

et al. 2014

Opt. Express

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“…Raster scanning of spherically focused detectors with central frequencies up to 50 MHz were used to obtain images of absorbers located close to the focus of the detector [8], and outside of the focus using synthetic aperture focusing techniques, based on the use of virtual detectors [9][10][11], or model-based algorithms [12]. Nonfocused detectors with 39 MHz low-pass cutoff frequency have been considered [13], but with low detection sensitivity due to the small detector size.…”

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confidence: 99%

Raster-scan optoacoustic mesoscopy in the 25–125 MHz range

2013

Self Cite

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We developed a raster-scan acoustic resolution broadband optoacoustic mesoscopy system and investigated the imaging performance using ultrasonic frequencies up to 125 MHz. The developed system achieves 7 μm axial resolution and transverse resolution of 30 μm reaching depths of at least 5 mm. This unprecedented performance is achieved by operating at out-of-focus ultrasonic detection and tomographic reconstruction. We demonstrate the limits reached due to the width of the laser pulse employed and showcase the technique on drosophila fly and drosophila pupae ex vivo.

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“…High spatial resolution is achieved using an ultrasonic transducer with a high central frequency, a wide bandwidth, and a high numerical aperture (NA) [10,11,19]. High-NA transducers have a wider angular coverage, which can be utilized in synthetic aperture focusing technique (SAFT) to improve the lateral resolution for out-of-focus regions in ARPAM systems [19][20][21][22][23][24]. However, high resolution still faces challenges in ARPAM.…”

Section: Introductionmentioning

confidence: 99%

In vivo deconvolution acoustic-resolution photoacoustic microscopy in three dimensions

Cai¹,

Li²,

Chen³

2016

Biomed. Opt. Express

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Acoustic-resolution photoacoustic microscopy (ARPAM) provides a spatial resolution on the order of tens of micrometers, and is becoming an essential tool for imaging fine structures, such as the subcutaneous microvasculature. High lateral resolution of ARPAM is achieved using high numerical aperture (NA) of acoustic transducer; however, the depth of focus and working distance will be deteriorated correspondingly, thus sacrificing the imaging range and accessible depth. The axial resolution of ARPAM is limited by the transducer's bandwidth. In this work, we develop deconvolution ARPAM (D-ARPAM) in three dimensions that can improve the lateral resolution by 1.8 and 3.7 times and the axial resolution by 1.7 and 2.7 times, depending on the adopted criteria, using a 20-MHz focused transducer without physically increasing its NA and bandwidth. The resolution enhancement in three dimensions by D-ARPAM is also demonstrated by in vivo imaging of the microvasculature of a chick embryo. The proposed D-ARPAM has potential for biomedical imaging that simultaneously requires high spatial resolution, extended imaging range, and long accessible depth.

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Fast scanning coaxial optoacoustic microscopy

Cited by 69 publications

References 15 publications

Multi-parametric quantitative microvascular imaging with optical-resolution photoacoustic microscopy in vivo

Multi-parametric quantitative microvascular imaging with optical-resolution photoacoustic microscopy in vivo

Raster-scan optoacoustic mesoscopy in the 25–125 MHz range

In vivo deconvolution acoustic-resolution photoacoustic microscopy in three dimensions

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