Fast-Track and Integration-Free Method of Genome Editing by CRISPR/Cas9 in Murine Pluripotent Stem Cells

Abstract

Graphical Abstract

Editorial: Complexity of tumor microenvironment: A major culprit in cancer development, volume II

“…Towards better functionality, genome editing tools like CRISPR/Cas9 and TALENs have made it possible to create additional genetic modifications in immune cells ( 36 , 38 , 39 ). Such methods have also been modified for better efficiency and safety in the therapeutic applications ( 40 , 41 ) although off-target and other hidden effects of genome editing are major concerns for their widespread routine use in clinics. Thus, more comprehensive studies related to the genetic modifications of immune and cancer cells are required to gain sufficient confidence for application as standard cancer therapy.…”