Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy

McConnell, Gail; Poland, Simon P.; Girkin, John M.

doi:10.1063/1.2148996

Cited by 14 publications

(13 citation statements)

References 21 publications

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“…We used a 0.7" XGA format DMD (Texas Instruments Ltd) mounted in a Discovery 1100 controller board (Vialux GmbH), with a mirror edge length of 13.68 µm. Several previous groups have used a DMD to produce custom spectra, by focussing a dispersed spectrum onto a DMD and then collecting the reflected light [5,8,9,13,14]. However the mirror tilt direction causes significant practical issues during system design, as a ray at normal incidence to the plane of the DMD is reflected through an angle of twice the mirror tilt out of the plane.…”

Section: System Design and Constructionmentioning

confidence: 99%

“…McConnell et al [8] attempted to use a DMD with a supercontinuum but did not use a second prism and only produced three distinct spectral bands. Chuang and Lo designed a system that could produce arbitrary profiles using an erbium-doped fibre amplifier with a central wavelength of 1550 nm and bandwidth of 37 nm [13].…”

Section: System Design and Constructionmentioning

confidence: 99%

“…Other groups have detailed the use of a supercontinuum as the light source, but these also required complex optics or had limited spectral resolution in the visible range [8,9].…”

Section: Introductionmentioning

confidence: 99%

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A tunable supercontinuum laser using a digital micromirror device

Wood¹,

Elson²

2012

Meas. Sci. Technol.

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Abstract. Spectral filtration systems based around Digital Micromirror Devices (DMD) have made possible the creation of illumination light with custom spectral profiles for microscopy and endoscopy. However due to the out-of-plane deviation caused by the DMD these systems have previously required complex optics, and in addition the diffraction effects of the DMD have not been well understood. We present a system that incorporates a Total-Internal Reflection (TIR) prism to significantly simplify the optics and also a full two-dimensional analysis of the diffraction patterns from the DMD. Full spectral coverage between 430 and 720 nm was demonstrated with the output coupled into a liquid light guide for easy coupling into surgical devices such as laparoscopes.

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Section: System Design and Constructionmentioning

confidence: 99%

Section: System Design and Constructionmentioning

confidence: 99%

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A tunable supercontinuum laser using a digital micromirror device

Wood¹,

Elson²

2012

Meas. Sci. Technol.

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“…These sources have provided much greater freedom in laser scanning microscopy, removing the previous restriction to laser lines (Dunsby et al, 2004;Betz et al, 2005;McConnell et al, 2006;Frank et al, 2007;Owen et al, 2007;Booth et al, 2008). However, demonstrations of these sources in fluorescence microscopy have all employed some form of wavelength filtering, so were unable to access the complete excitation/emission spectra in a single exposure.…”

Section: Introductionmentioning

confidence: 99%

Full spectrum filterless fluorescence microscopy

Booth

Jesacher

Juškaitis

et al. 2009

Journal of Microscopy

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SummaryIn conventional microscopes, fluorescence emission is separated from the backscattered illumination using the Stokes shift, whereby the emission occurs at a longer wavelength to the excitation. Such separation is usually achieved through a combination of wavelength filters that divide the spectrum into mutually exclusive excitation and emission bands. It is therefore impossible in these microscopes to access the full excitation/emission spectrum of the specimen in a single image. We report on a microscope that acquired fluorescence images using illumination across the spectral range 450-680 nm; the full emission spectrum was detected simultaneously across the same range. The microscope was also combined with structured illumination optical sectioning to give three-dimensionally resolved images with improved background rejection. Full spectrum fluorescence images of biological specimens are demonstrated. As this system is more versatile than the standard fluorescence microscope, it could be of benefit in many fluorescence imaging applications.

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“…The DMD is used to divert subsets of the microscope image, corresponding to multiple points of interest selected from the full field-of-view, to a spectrometer. DMDs, consisting of hundreds of thousands of individually addressable moving micromirrors, were originally developed for the display industry [16] but have also found applications in other areas including wavelength multiplexing [17]. They offer a method of modulating light which is fast, highly efficient and works over a broad range of wavelengths.…”

Section: Introductionmentioning

confidence: 99%

A multi-object spectral imaging instrument

Gibson

Dienerowitz

Kelleher

et al. 2013

J. Opt.

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We have developed a snapshot spectral imaging system which fits onto the side camera port of a commercial inverted microscope. The system provides spectra, in real time, from multiple points randomly selected on the microscope image. Light from the selected points in the sample is directed from the side port imaging arm using a digital micromirror device to a spectrometer arm based on a dispersing prism and CCD camera. A multi-line laser source is used to calibrate the pixel positions on the CCD for wavelength. A CMOS camera on the front port of the microscope allows the full image of the sample to be displayed and can also be used for particle tracking, providing spectra of multiple particles moving in the sample. We demonstrate the system by recording the spectra of multiple fluorescent beads in aqueous solution and from multiple points along a microscope sample channel containing a mixture of red and blue dye.

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Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy

Cited by 14 publications

References 21 publications

A tunable supercontinuum laser using a digital micromirror device

A tunable supercontinuum laser using a digital micromirror device

Full spectrum filterless fluorescence microscopy

A multi-object spectral imaging instrument

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