A multi-object spectral imaging instrument

Gibson, Graham M.; Dienerowitz, Maria; Kelleher, P. A.; Harvey, Andrew R.; Padgett, Miles J.

doi:10.1088/2040-8978/15/8/085302

Cited by 9 publications

(5 citation statements)

References 24 publications

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“…Increasing the excitation power results in an equivalent increase in background noise, even more than in the fluorescent case, since we are not filtering out the excitation laser. Alternative approaches include spatial filtering or using the nanoparticle's plasmon luminescence [49][50][51][52][53] . We investigated the effects of axial positioning and varying trapping area size by adjusting the diameter of the laser scan pattern (see Fig.…”

Section: Resultsmentioning

confidence: 99%

Confining Brownian motion of single nanoparticles in an ABELtrap

et al. 2017

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Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap (pioneered by A. E. Cohen and W. E. Moerner [1][2][3][4][5][6][7] ) to hold nanoparticles and individual FoF1-ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object's Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap.

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Section: Resultsmentioning

confidence: 99%

Confining Brownian motion of single nanoparticles in an ABELtrap

et al. 2017

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“…For Raman measurements, the samples were measured on either a MgF 2, CaF 2, or quartz coverslips (0.17 mm thick) mounted to a custom-built sample holder. The Raman back-scattered light was collected by the same objective and focused onto a DMD (Texas Instruments DLP Lightcrafter evaluation board, Farnell UK), modified to remove LEDs and projection optics (as described in [ 21 ]). A binary image displayed on the DMD display determines which direction the light is reflected at each pixel, either + 12° or −12° from normal.…”

Section: Methodsmentioning

confidence: 99%

“…In the first step, confocal AF imaging was used to generate sampling points for the multifocal RMS. While a LC-SLM was utilized for creating a power-shared excitation pattern for RMS (similar to [ 18 – 20 ]), the spectrometer slit was removed and a digital micro-mirror device (DMD) was added to behave as a software-reconfigurable reflective pseudo-“slit”, as demonstrated in [ 21 ] for fluorescence spectroscopy. While DMDs have been previously used in Raman micro-spectroscopy as spectral modulators replacing the CCD [ 22 ] or for spatially-offset Raman spectroscopy [ 23 ], here the DMD is used in a novel way as a software configurable multi-slit pattern.…”

Section: Introductionmentioning

confidence: 99%

Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging

Sinjab

Kong

Gibson

et al. 2016

Biomed. Opt. Express

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We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous “power-sharing” acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be rapidly updated in real-time via software control of a liquid-crystal spatial light modulator (LC-SLM), this technique is compatible with automated adaptive- and selective-sampling Raman spectroscopy techniques, the latter of which has previously been demonstrated for fast diagnosis of skin cancer tissue resections. We describe the performance of this instrument and show examples of multiplexed measurements on a range of test samples. Following this, we show the feasibility of reducing measurement time for power-shared multifocal Raman measurements combined with confocal auto-fluorescence imaging to provide guided diagnosis of tumours in human skin samples.

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“…Amplitude modulation with DMDs has been used for a variety of applications in optics, from single-pixel compressive sensing cameras 15,16 and spatially encoded fluorescence spectroscopic imaging, 17 to their use as computer-controlled reflective apertures. 18 Many of these optical applications have focused on bright-field and fluorescence microscopy, where DMDs can modify the light fields in some desirable way as shown in Fig. 1d-f, to improve aspects of measurement such as speed or spatial resolution.…”

Section: Introductionmentioning

confidence: 99%

Applications of Spatial Light Modulators in Raman Spectroscopy

2019

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Advances in consumer display screen technologies have historically been adapted by researchers across the fields of optics as they can be used as electronically controlled spatial light modulators (SLMs) for a variety of uses. The performance characteristics of such SLM devices based on liquid crystal (LC) and digital micromirror device (DMD) technologies, in particular, has developed to the point where they are compatible with increasingly sensitive instrumental applications, for example, Raman spectroscopy. Spatial light modulators provide additional flexibility, from modulation of the laser excitation (including multiple laser foci patterns), manipulation of microscopic samples (optical trapping), or selection of sampling volume (adaptive optics or spatially offset Raman spectroscopy), to modulation in the spectral domain for high-resolution spectral filtering or multiplexed/compressive fast detection. Here, we introduce the benefits of different SLM devices as a part of Raman instrumentation and provide a variety of recent example applications which have benefited from their incorporation into a Raman system.

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A multi-object spectral imaging instrument

Cited by 9 publications

References 24 publications

Confining Brownian motion of single nanoparticles in an ABELtrap

Confining Brownian motion of single nanoparticles in an ABELtrap

Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging

Applications of Spatial Light Modulators in Raman Spectroscopy

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