Fast weight recovery, metabolic rate adjustment and gene-expression regulation define responses of cold-stressed honey bee brood

Ramírez, Leonor; Luna, Facundo; Mucci, Claudio Andoni; Lamattina, Lorenzo

doi:10.1016/j.jinsphys.2020.104178

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…In A. m. carnica , most of significant GO terms seems to be linked with stress tolerance, notably response to wounding, reactive oxygen species (ROS) metabolism, and larval midgut programmed cell death ( Figure 6 ). More precisely, several significant GO terms are likely involved in cold resistance in honeybees, such as developmental growth [ 69 ], ROS metabolism [ 70 ], and hippo signaling [ 13 ]. Interestingly, the gene encoding the protein Dachsous, which was under positive selection in A. m. carnica and found in several significant GO terms, plays a key role in the adaptation to temperate climate in the A. m. sinisxinyuan [ 13 ].…”

Section: Resultsmentioning

confidence: 99%

Signatures of Positive Selection in the Genome of Apis mellifera carnica: A Subspecies of European Honeybees

Huang

Zhu

Fouks

et al. 2022

Life

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The technology of long reads substantially improved the contingency of the genome assembly, particularly resolving contiguity of the repetitive regions. By integrating the interactive fragment using Hi-C, and the HiFi technique, a solid genome of the honeybee Apis mellifera carnica was assembled at the chromosomal level. A distinctive pattern of genes involved in social evolution was found by comparing it with social and solitary bees. A positive selection was identified in genes involved with cold tolerance, which likely underlies the adaptation of this European honeybee subspecies in the north hemisphere. The availability of this new high-quality genome will foster further studies and advances on genome variation during subspeciation, honeybee breeding and comparative genomics.

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Section: Resultsmentioning

confidence: 99%

Signatures of Positive Selection in the Genome of Apis mellifera carnica: A Subspecies of European Honeybees

Huang

Zhu

Fouks

et al. 2022

Life

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“…This suggests that the level of disturbance due to monitoring is not affected by the methodology per se, but on the handling by the observer. Unexperienced observers spend more time on each assessment, increasing the colony stress by preventing the colony from a faster return to their original state (before the stress), and by increasing the brood temperature fluctuations, which, above certain levels, cannot allow the brood to recover from the stressor (Ramirez et al, 2021). Also, the gentleness used in frame handling and brushing the bees can play a role in decreasing these stressors.…”

Section: Discussionmentioning

confidence: 99%

High accuracy monitoring of honey bee colony development by a quantitative method

Capela

Dupont

Rortais

et al. 2022

Journal of Apicultural Research

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Honey bees are key insect pollinators, providing important economic and ecological value for human beings and ecosystems. This has triggered the development of several monitoring methods for assessing the temporal development of colony size, food storage, brood and pathogens. Nonetheless, most of these methods are based on visual assessments that are observer-dependent and prone to bias. Furthermore, the impact on colony development (invasiveness), as well as accuracy, were rarely considered when implementing new methods. In this study, we present and test a novel accurate and observer-independent method for honey bee colony assessment, capable of being fully standardized. Honey bee colony size is quantified by assessing the weight of adult bees, while brood and provision are assessed by taking photos and conducting image analysis of the combs with the image analysis software DeepbeeV R . The invasiveness and accuracy of the method were investigated using field data from two experimental apiaries in Portugal, comparing results from test and control colonies. At the end of each field experiment, most of the tested colonies had the same colony size, brood levels and honey production as the control colonies. Nonetheless, continuous weight data indicated some disturbance in tested colonies in the first year of monitoring. The overall accuracy of the image analysis software was improved by training, indicating that it is possible to adapt the software to local conditions. We conclude that the use of this fully quantitative method offers a more accurate alternative to classic visual colony assessments, with negligible impact on colony development.

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“…Adipokinetic hormone (AKH) is one of the primary neuroendocrine regulators of lipid metabolism in insects, and while a direct role for this hormone in cold stress has not been identified, genes encoding its receptor (AkhR) are activated by low temperatures. AkhR transcripts are upregulated in both D. suzukii after 9 d of cold acclimation at 10°C (Enriquez and Colinet, 2019b) and after three days of decreased temperatures (from 34 to 25°C) in brood of Apis melifera (Ramirez et al, 2021), suggesting increased sensitivity to AKH may be required to maintain lipid metabolism at low temperatures. AKH is also involved in regulating supercooling capacity in some insects.…”

Section: -Molecular Regulation Of Lipid Metabolism During Cold Exposurementioning

confidence: 99%

Lipid Metabolism in Response to Cold

Enriquez¹,

Teets²

2023

Preprint

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Temperature directly shapes insect physiology and has a preponderant effect on life history traits. Winter conditions in temperate and polar regions are especially challenging for insects. Extremely low temperatures can indeed compromise insect survival by promoting freezing of body fluids, but mild cold temperatures above 0 °C (i.e. chilling) can also lead to complex and severe physiological dysregulations. Among physiological damages due to freezing and chilling, insect lipids are one of the primary targets. As low temperatures tend to rigidify phospholipid bilayers, membranes functions are compromised at cold. Lipid rigidification due to cold also decreases the accessibility of fat stores for metabolic enzymes, and therefore their availability for basal metabolism. These deleterious effects, combined with low food availability in winter, result in a substantial nutritional challenge for overwintering insects. Consequently, lipid modifications such as homeoviscous adaptation of cell membranes, fluidity maintenance of fat reserves, cuticular lipid accumulation, and production of antifreeze glyclolipids are essential components of the physiological response to cold stress. The aim of the present chapter is to present the physiological challenges caused by low temperatures, the lipid modifications linked with cold tolerance in insects, and the molecular regulation of lipid metabolism during cold exposure.

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Fast weight recovery, metabolic rate adjustment and gene-expression regulation define responses of cold-stressed honey bee brood

Cited by 8 publications

References 32 publications

Signatures of Positive Selection in the Genome of Apis mellifera carnica: A Subspecies of European Honeybees

Signatures of Positive Selection in the Genome of Apis mellifera carnica: A Subspecies of European Honeybees

High accuracy monitoring of honey bee colony development by a quantitative method

Lipid Metabolism in Response to Cold

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