“…Bench scale denitrification tests using glycerin as a carbon source have reported 1) nitrite accumulation (Onnis-Hayden, 2011;Uprety, 2012;Bilyk, 2012) and 2) glycerin-COD storage and increasing SDNR and/or COD:N requirements over time (Bilyk, 2011, Uprety, 2012 perhaps due to the development of specialized populations or adaptation of enzymatic activities based on the environment the microbial community is exposed to.…”