Fasting and refeeding induces changes in the mouse hepatic lipid droplet proteome

Kramer, David A.; Quiroga, Ariel D.; Lian, Jihong; Fahlman, Richard P.; Lehner, Richard

doi:10.1016/j.jprot.2018.04.024

Cited by 38 publications

(21 citation statements)

References 59 publications

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“…In agreement with these observations, SNAP23 expression increases in rat livers during fasting [217]. Proteomic analysis of purified LDs from mouse livers revealed that PLIN5 and ACSL1 proteins are more abundant in fasted mice [218]. It is likely that the enhanced mitochondria-LD contacts favor fatty acid oxidation in mitochondria under conditions of nutrient deprivation or during physiological fasting.…”

Section: Mitochondria-ld Contacts Respond To Metabolic Alterationssupporting

confidence: 72%

Metabolic implications of organelle–mitochondria communication

2019

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Cellular organelles are not static but show dynamism—a property that is likely relevant for their function. In addition, they interact with other organelles in a highly dynamic manner. In this review, we analyze the proteins involved in the interaction between mitochondria and other cellular organelles, especially the endoplasmic reticulum, lipid droplets, and lysosomes. Recent results indicate that, on one hand, metabolic alterations perturb the interaction between mitochondria and other organelles, and, on the other hand, that deficiency in proteins involved in the tethering between mitochondria and the ER or in specific functions of the interaction leads to metabolic alterations in a variety of tissues. The interaction between organelles is an emerging field that will permit to identify key proteins, to delineate novel modulation pathways, and to elucidate their implications in human disease.

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Section: Mitochondria-ld Contacts Respond To Metabolic Alterationssupporting

confidence: 72%

Metabolic implications of organelle–mitochondria communication

2019

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“…Proteins harboring hydrophobic domains decorate lipid droplet and lipoprotein monolayers: the lipoprotein proteome mainly consists of apolipoproteins and lipoprotein-associated proteins that regulate lipid metabolism, whereas the lipid droplet proteome is highly diverse. Proteomic-and microscopy-based studies have identified proteins involved in cellular trafficking, key signaling proteins, transcription factors, and histones on the surface of lipid droplets, in addition to proteins regulating lipid metabolism and flux (9)(10)(11). These differences between lipid droplets and lipoproteins relate to their different functions in hepatic lipid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Hepatic lipid droplet homeostasis and fatty liver disease

Seebacher

Zeigerer²,

Kory³

et al. 2020

Seminars in Cell & Developmental Biology

127

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In cells, lipids are stored in lipid droplets, dynamic organelles that adapt their size, abundance, lipid and protein composition and organelle interactions to metabolic changes. Lipid droplet accumulation in the liver is the hallmark of non-alcoholic fatty liver disease (NAFLD). Due to the prevalence of obesity, the strongest risk factor for steatosis, NAFLD and its associated complications are currently affecting more than 1 billion people worldwide. Here, we review how triglyceride and phospholipid homeostasis are regulated in hepatocytes and how imbalances between lipid storage, degradation and lipoprotein secretion lead to NAFLD. We discuss how organelle interactions are altered in NAFLD and provide insights how NAFLD progression is associated with changes in hepatocellular signaling and organ-crosstalk. Finally, we highlight unsolved questions in hepatic LD and lipoprotein biology and give an outlook on therapeutic options counteracting hepatic lipid accumulation.

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“…During fasting, which also stimulates lipolysis but is not associated with vigorous muscle contraction, skeletal muscle actually accumulates TAG as seen in laboratory animals by biochemical analysis [47] and in human subjects by measuring intramyocellular lipid by MRS [48][49][50][51][52]. As with exercise, fasting leads to accumulation of TAG in the liver as observed in rodent studies [53][54][55][56] and accumulation of intrahepatocellular lipid (presumably mostly TAG) as observed by non-invasive MRS in human subjects research [57]. It is important to keep in mind that the acute response to each bout of a stressor is not necessarily qualitatively similar to the chronic stress response.…”

Section: Discussionmentioning

confidence: 99%

Acute exercise in mice transiently remodels the hepatic lipidome in an intensity-dependent manner

2020

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Background The content of triacylglycerol (TAG) in the liver is known to rapidly increase after a single bout of exercise followed by recovery to sedentary levels. The response of other hepatic lipids, and acyl chain composition of lipid classes, would provide a deeper understanding of the response of hepatic lipid metabolism to acute exercise. Methods Female mice performed a single bout of continuous exercise (CE), high-intensity interval exercise (HIIE), or no exercise (CON). The total content of various lipids in the liver, and fatty acids within lipid classes, were measured in tissues collected 3 h after exercise (Day 1) and the day following exercise (Day 2). Results The total concentration of TAG rose on Day 1 after exercise (P < 0.05), with a greater elevation in HIIE than CE (P < 0.05), followed by a decline toward CON levels on Day 2. The total concentration of other measured lipid classes was not significantly altered by exercise. However, n-6 polyunsaturated fatty acid relative abundance in diacylglycerol (DAG) was increased by HIIE (P < 0.05). In CON liver, TAG content was positively correlated with DAG and phosphatidylethanolamine (P < 0.05), while these statistical associations were disrupted in exercised mice on Day 1. Conclusions The response of lipid metabolism to exercise involves the coordination of metabolism between various tissues, and the lipid metabolism response to acute exercise places a metabolic burden upon the liver. The present findings describe how the liver copes with this metabolic challenge. The flexibility of the TAG pool size in the liver, and other remodeling of the hepatic lipidome, may be fundamental components of the physiological response to intense exercise.

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Fasting and refeeding induces changes in the mouse hepatic lipid droplet proteome

Cited by 38 publications

References 59 publications

Metabolic implications of organelle–mitochondria communication

Metabolic implications of organelle–mitochondria communication

Hepatic lipid droplet homeostasis and fatty liver disease

Acute exercise in mice transiently remodels the hepatic lipidome in an intensity-dependent manner

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