Fatal Acinetobacter baumanii Pneumonia Diagnosed at Autopsy in a Patient with End-Stage Renal Disease

Mourtzinos, Nikki; Schwartz, Arnold M.; Orenstein, Jan M.

doi:10.1097/pcr.0b013e31805d0b6e

Cited by 2 publications

(1 citation statement)

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“…In addition, AbOmpA, the effector molecule inducing host cell death (Choi et al , 2005, 2008b), was mainly secreted from A. baumannii through OMVs. Recently, Mourtzinos et al (2007) reported that A. baumannii secreted pleomorphic vesicles in infected lung tissues, suggesting the possibility of secretion of OMVs in vivo infection.…”

Section: Resultsmentioning

confidence: 99%

Proteome analysis of outer membrane vesicles from a clinicalAcinetobacter baumanniiisolate

Kwon¹,

Gho²,

Lee³

et al. 2009

FEMS Microbiology Letters

149

139

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The secretion of outer membrane vesicles (OMVs) is one of the major mechanisms by which Gram-negative bacteria deliver effector molecules to host cells. Acinetobacter baumannii is an important opportunistic pathogen in hospital-acquired infections, but the secretion system for effector molecules to induce host cell damage has not been characterized. In the present study, we investigated the secretion of OMVs from a clinical A. baumannii isolate and analyzed the comprehensive proteome of A. baumannii-derived OMVs. Acinetobacter baumannii secreted OMVs into the extracellular milieu during in vitro growth. Using 1-DE and LC-MS/MS protein identification and assignment analysis, 132 different proteins associated with OMVs were identified. These proteins were derived from outer membranes (n=26), periplasmic space (n=6), inner membranes (n=8), cytoplasm (n=43), and unknown localization or multiple localization sites (n=49) according to the cell location prediction programs. Among the proteins associated with OMVs, a potent cytotoxic molecule, outer membrane protein A, was highly enriched and several putative virulence-associated proteins were also identified. These results suggest that OMVs from A. baumannii are an important vehicle designed to deliver effector molecules to host cells.

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Section: Resultsmentioning

confidence: 99%