Fatal Nosocomial Spread of Crimean-Congo Hemorrhagic Fever with Very Short Incubation Period

Naderi, HamidReza; Sheybani, Fereshte; Bojdi, Amin; Khosravi, Nasim; Mostafavi, Irandokht

doi:10.4269/ajtmh.2012.12-0337

Cited by 38 publications

(19 citation statements)

References 11 publications

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“…Despite serological evidence of infection in Iran reported in 1975, CCHFV has recently received much more attention as a potential threat to public health by health authorities since an outbreak in 1999 [11,30]. The risk of CCHFV to the community is highlighted by a report of a nosocomial outbreak in Mashhad in northwestern Iran, which was found to be associated with inadequate self-care procedures of a physician during the handling of CCHF patients [31].…”

Section: Discussionmentioning

confidence: 98%

Expression and characterization of codon-optimized Crimean-Congo hemorrhagic fever virus Gn glycoprotein in insect cells

et al. 2017

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Crimean-Congo hemorrhagic fever virus (CCHFV) is a major cause of tick-borne viral hemorrhagic disease in the world. Despite of its importance as a deadly pathogen, there is currently no licensed vaccine against CCHF disease. The attachment glycoprotein of CCHFV (Gn) is a potentially important target for protective antiviral immune responses. To characterize the expression of recombinant CCHFV Gn in an insect-cell-based system, we developed a gene expression system expressing the full-length coding sequence under a polyhedron promoter in Sf9 cells using recombinant baculovirus. Recombinant Gn was purified by affinity chromatography, and the immunoreactivity of the protein was evaluated using sera from patients with confirmed CCHF infection. Codon-optimized Gn was successfully expressed, and the product had the expected molecular weight for CCHFV Gn glycoprotein of 37 kDa. In time course studies, the optimum expression of Gn occurred between 36 and 48 hours postinfection. The immunoreactivity of the recombinant protein in Western blot assay against human sera was positive and was similar to the results obtained with the anti-V5 tag antibody. Additionally, mice were subjected to subcutaneous injection with recombinant Gn, and the cellular and humoral immune response was monitored. The results showed that recombinant Gn protein was highly immunogenic and could elicit high titers of antigen-specific antibodies. Induction of the inflammatory cytokine interferon-gamma and the regulatory cytokine IL-10 was also detected. In conclusion, a recombinant baculovirus harboring CCHFV Gn was constructed and expressed in Sf9 host cells for the first time, and it was demonstrated that this approach is a suitable expression system for producing immunogenic CCHFV Gn protein without any biosafety concerns.

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Section: Discussionmentioning

confidence: 98%

Expression and characterization of codon-optimized Crimean-Congo hemorrhagic fever virus Gn glycoprotein in insect cells

et al. 2017

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“…Slične epidemije su opisane u Turskoj 2012. godine. U epidemiji u Iranu (2012) je oboleo student medicine koji se razboleo 20 sati nakon kontakta s obolelim od KKHG, a nakon 7 dana bolest se kod njega završila letalno [2,4,17,18,22].…”

Section: Diskusijaunclassified

“…Takođe je veoma značajna pravilna interpretacija seroloških testova jer se virus-specifična antitela javljaju sedmog do desetog dana bolesti, dok se neutrališuća antitela javljaju nakon četrnaest do šesnaest dana, te ukoliko je krv za analizu uzeta ranije, nalaz će biti lažno negativan [2,22,23]. U našoj porodičnoj epidemiji u kojoj je obolelo 6 članova, virus specifična antitela su dokazana kod dva bolesnika, dok kod ostalih nisu jer je krv testirana prvih dana bolesti kada se antitela još nisu pojavila.…”

Section: Diskusijaunclassified

Crimean Congo hemorrhagic fever - description of family epidemic

Katanić

Katanic

Mirić

et al. 2013

Biomedicinska istraž.

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Background. Crimean Congo hemorrhagic fever (CCHF) is an acute, viral, natural, focal zoonosis, which is under natural conditions transferred to man by the bite of certain species of ticks. There is also inter-human transmission of infection. A clinical picture is characterized by temperature and severe hemorrhages of different localization and different intensity. In this paper family epidemics of Crimean-Congo hemorrhagic fever are described. Epidemic description. Treating patients with Crimean-Congo hemorrhagic fever in Clinic of Infectious Diseases in Pristina we observed six family outbreaks. In four epidemics two family members were affected, in one four and in the other six members. In all patients the disease was manifested with temperature and bleeding. In both families in which four and six people were affected, one lethal outcome appeared. Diagnosis of Crimean-Congo hemorrhagic fever was made on the basis of epidemiological, clinical and serological data. Conclusion. Crimean-Congo hemorrhagic fever is characterized by fever and hemorrhages. It occurs sporadically or in small, often family outbreaks. Although it is a zoonosis, inter-human transmission occurs, which is of great epidemiological importance.

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“…A more recent detailed literature review of nosocomial CCHF transmission to HCWs listed 44 infections in 494 HCW contacts in 12 countries (Tarantola et al, 2007). Nosocomial infections were reported from South Africa Shepherd et al, 1985;Vandewal et al, 1985;Vaneeden et al, 1985a, b), Mauretania (Nabeth et al, 2004), Sudan (Elata et al, 2011), Albania (Harxhi et al, 2005;Papa et al, 2001), Kosovo (Papa et al, 2002), Bulgaria (Kunchev & Kojouharova, 2008;Papa et al, 2004), Turkey (Celikbas et al, 2014;Gürbüz et al, 2009), Iran (Chinikar et al, 2013;Mardani et al, 2009;Naderi et al, 2013), Dubai (Suleiman et al, 1980), Pakistan (Burney et al, 1980;Hasan et al, 2013), India , Tajikistan (ProMED-mail, 2009b), Kazakhstan (ProMED-mail, 2009a) and Germany (Conger et al, 2015).…”

Section: Nosocomial Cchf Infectionsmentioning

confidence: 99%