Fate of Adipose-Derived Stromal Vascular Fraction Cells after Co-implantation with Fat Grafts

Abstract

The authors show convincing evidence for dynamic changes of stromal vascular fraction cells after co-implantation with fat grafts. The results prove the principle that implanted stromal vascular fraction cells can survive in the ischemic microenvironment of fat grafts and participate in the process of adipogenesis and angiogenesis.

Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts at the Early Stage and Improve Long-Term Retention in Immunocompetent Rats

“…In this study, the protein of Perilipin A coating lipid droplets in adipocytes was applied to assess the injury degree of adipocytes. Only the healthy adipocytes were Perilipin A-positive, and therefore we were able to easily discriminated living and dead adipocytes timely and accurately [29]. As a result, the number of dead adipocytes in the ALLG group was less than in the AFT group at 7 and 14 days.…”

“…ASCs can promote vascularization by its paracrine functions and multipotential differentiation [27] .They have the property of secreting various proangiogenic cytokines, such as vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), insulin growth factor (IGF), and leptin [28]. Moreover, ASCs can also directly differentiate into endothelial cells to promote neovascularization besides the paracrine mechanism [29]. In this study, as shown by CD34 immunofluorescence, syngeneic ASCs increased the capillary density in agreement with previous studies [10,26].…”

Improvement of Fat Graft Survival with Autologous Bone Marrow Aspirate and Bone Marrow Concentrate: A One-Step Method

“…Stromal vascular fraction cells can survive in the local region of fat injection for a long time, differentiate into adipocytes, and participate in vascular regeneration. 24 However, the mechanism for the sharp decrease of cells we observed is still obscure. The implanted fat tissues suffer from ischemia and hypoxia during the early stages after implantation.…”

In Vivo Bioimaging Analysis of Stromal Vascular Fraction-Assisted Fat Grafting

“…The result from this study showed that, compared to the fat grafts without SVF, fat tissues transplanted with SVF had higher survival rate since 14 days after injection (P=0.001). Moreover, stem or progenitor cells in SVFs are believed to be helpful in tissue regeneration by differentiation (34). At the end of 63 days of follow-up, SVF group had a significant advantage in volume of survived fat tissue (PG0.001).…”