Fate of antibiotic resistance genes and metal resistance genes during the thermophilic fermentation of solid and liquid swine manures in an ectopic fermentation system

Shen, Qi; Tang, Jiangwu; Wang, Xin; Li, Yuancheng; Yao, Xin; Sun, Hong; Wu, Yingliang

doi:10.1016/j.ecoenv.2021.111981

Cited by 35 publications

(14 citation statements)

References 40 publications

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“…However, for the long-term period, the relative abundance of czcA in the Cd 5 mg L −1 + DC 1 mg L −1 treatment was relatively high, accompanied by higher relative abundances of tetA, tetG, and intI1 (Figure 3). We hypothesized that the relative abundance of czcA increase was principally due to co-resistance via ARGs or enhancement of horizontal transfer potential via intI1 (Shen et al, 2021). This speculation was confirmed by the results of RDA, which showed that czcA was significantly positively associated with tetA, tetG, and intI1 (Figure 5).…”

Section: Evolutions Of Antibiotic Resistance Genes Metal Resistance G...mentioning

confidence: 67%

Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Shu

et al. 2022

Front. Microbiol.

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Abuse of heavy metals and antibiotics results in the dissemination of metal resistance genes (MRGs) and antibiotic resistance genes (ARGs). Ditch wetlands are important sinks for heavy metals and antibiotics. The relationships between bacterial communities and MRG/ARG dissemination under dual stresses of heavy metals and antibiotics remain unclear. The responses of MRGs and ARGs to the co-selective pressure of cadmium (Cd) and doxycycline (DC) in ditch wetlands were investigated after 7-day and 84-day exposures. In ecological ditches, residual rates of Cd and DC varied from 0.4 to –5.73% and 0 to –0.61%, respectively. The greatest total relative abundance of ARGs was observed in the Cd 5 mg L–1 + DC 50 mg L–1 group. A significant level of DC (50 mg L–1) significantly reduced the total relative abundances of MRGs at a concentration of 5 mg L–1 Cd stress. Redundancy analysis indicated that Cd and DC had strong positive effects on most ARGs and MRGs after a 7-day exposure. Meanwhile, the class 1 integron gene (intI1) exhibited strong positive correlations with most ARGs and cadmium resistance genes (czcA) after an 84-day exposure. Network analysis showed that Acinetobacter and Pseudomonas were the potential dominant host genera for ARGs and MRGs, and tetracycline resistance genes (tetA), czcA, and intI1 shared the same potential host bacteria Trichococcus after an 84-day exposure.

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Section: Evolutions Of Antibiotic Resistance Genes Metal Resistance G...mentioning

confidence: 67%

Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Shu

et al. 2022

Front. Microbiol.

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“…Anaerobic digestion has been shown to reduce the tetracycline resistance genes in manure but failed to eliminate it or the tetracycline residues [80]. Ectopic fermentation systems were tested and reduced the abundance of tetracycline resistance genes and metal resistance genes in manure [81]. The use of microbial agents during composting can also reduce the number of ARGs and antibiotic residues [75,82].…”

Section: From Pig To Environmentmentioning

confidence: 99%

“…According to current data, one treatment cannot be used to reduce the ARGs of all classes of antibiotics, nor can it be used to completely remove ARGs of a given class. Composting manure before using it as a fertilizer might contribute to reduce the spread of ARGs from the farm to the environment [75,81,82]. However, more studies are required to properly assess the impact of composted manure on soils.…”

Section: From Pig To Environmentmentioning

confidence: 99%

Antibiotic Resistance: From Pig to Meat

et al. 2021

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Pork meat is in high demand worldwide and this is expected to increase. Pork is often raised in intensive conditions, which is conducive to the spread of infectious diseases. Vaccines, antibiotics, and other biosafety measures help mitigate the impact of infectious diseases. However, bacterial strains resistant to antibiotics are more and more frequently found in pig farms, animals, and the environment. It is now recognized that a holistic perspective is needed to sustainably fight antibiotic resistance, and that an integrated One Health approach is essential. With this in mind, this review tackles antibiotic resistance throughout the pork raising process, including their microbiome; many factors of their environment (agricultural workers, farms, rivers, etc.); and an overview of the impact of antibiotic resistance on pork meat, which is the end product available to consumers. Antibiotic resistance, while a natural process, is a public health concern. If we react, and act, collectively, it is expected to be, at least partially, reversible with judicious antibiotic usage and the development of innovative strategies and tools to foster animal health.

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“…In the last few years, an improved method for decomposition of poultry manure appeared: ectopic fermentation systems (EFS) 17,18 . Fermentation tanks, built near the breeding room, can be filled with padding materials and feces collected from dairy farms.…”

Section: Introductionmentioning

confidence: 99%

Treatment of piggery waste in an ectopic microbial fermentation system and safety evaluation of generated organic fertilizer

Liu

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND With the development of China’s livestock breeding industry, a large amount of waste was discharged and the local environment was seriously polluted. In this research, treatment performance of piggery waste in an ectopic fermentation system (EFS) using different padding materials and safety evaluation of generated organic fertilizer were studied in a pilot experiment. RESULTS During the whole fermentation process, temperature in the EFS fluctuated with addition of fresh feces and its consumption, and the pH value varied between 6.5 and 9.5. The absorption coefficient of pig breeding wastewater and feces per kilogram of padding (maize straw) at the end was 2.52 and 1.88, respectively. Bacteria played a major role in decomposing organic matter, and its number was basically maintained at 107–108 orders of magnitude. The C:N ratio in two EFS beds decreased gradually and was below 14:1 at the end, indicating the maturity of fermentation. Moreover, operation of the EFS with addition of rice husk/coconut shell mixture was more stable than that of EFS with maize straw. After fermentation, the heavy metal, total nutrient content, ascaris egg mortality and fecal coliform population of the generated organic fertilizer from two fermentation beds all met the limits of the Organic Fertilizer Standard of China, and the seed germination index of the product increased. CONCLUSIONS EFS employing maize straw or rice husk/coconut shell mixture as padding material can be used to treat both solid and liquid waste from pig farms. EFS technology reduced the biotoxicity of piggery waste. © 2022 Society of Chemical Industry (SCI).

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Fate of antibiotic resistance genes and metal resistance genes during the thermophilic fermentation of solid and liquid swine manures in an ectopic fermentation system

Cited by 35 publications

References 40 publications

Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Antibiotic Resistance: From Pig to Meat

Treatment of piggery waste in an ectopic microbial fermentation system and safety evaluation of generated organic fertilizer

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