Fate of petroleum hydrocarbons taken up from food and water by the blue crab Callinectes sapidus

Lee, R. F.; Ryan, C. W.; Neuhauser, M. L.

doi:10.1007/bf00387492

Cited by 114 publications

(27 citation statements)

References 17 publications

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“…ΣPAH in viscera were 5 to 20 times higher than in tails (Figure 1A). This is consistent with previous work, which observed that PAH levels are highest in the hepatopancreas and lowest in muscle tissue in crabs (Lee et al, 1976). In crayfish, it has been suggested that oxidative metabolism of PAHs and other xenobiotics occurs in the hepatopancreas and in the green gland (James and Boyle, 1998; Jewell et al, 1997).…”

Section: Resultssupporting

confidence: 94%

Passive samplers accurately predict PAH levels in resident crayfish

Paulik

Smith

Bergmann

et al. 2016

Science of The Total Environment

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Contamination of resident aquatic organisms is a major concern for environmental risk assessors. However, collecting organisms to estimate risk is often prohibitively time and resource-intensive. Passive sampling accurately estimates resident organism contamination, and it saves time and resources. This study used low density polyethylene (LDPE) passive water samplers to predict polycyclic aromatic hydrocarbon (PAH) levels in signal crayfish, Pacifastacus leniusculus. Resident crayfish were collected at 5 sites within and outside of the Portland Harbor Superfund Megasite (PHSM) in the Willamette River in Portland, Oregon. LDPE deployment was spatially and temporally paired with crayfish collection. Crayfish visceral and tail tissue, as well as water-deployed LDPE, were extracted and analyzed for 62 PAHs using GC-MS/MS. Freely-dissolved concentrations (Cfree) of PAHs in water were calculated from concentrations in LDPE. Carcinogenic risks were estimated for all crayfish tissues, using benzo[a]pyrene equivalent concentrations (BaPeq). ΣPAH were 5–20 times higher in viscera than in tails, and ΣBaPeq were 6–70 times higher in viscera than in tails. Eating only tail tissue of crayfish would therefore significantly reduce carcinogenic risk compared to also eating viscera. Additionally, PAH levels in crayfish were compared to levels in crayfish collected 10 years earlier. PAH levels in crayfish were higher upriver of the PHSM and unchanged within the PHSM after the 10-year period. Finally, a linear regression model predicted levels of 34 PAHs in crayfish viscera with an associated R-squared value of 0.52 (and a correlation coefficient of 0.72), using only the Cfree PAHs in water. On average, the model predicted PAH concentrations in crayfish tissue within a factor of 2.4 ± 1.8 of measured concentrations. This affirms that passive water sampling accurately estimates PAH contamination in crayfish. Furthermore, the strong predictive ability of this simple model suggests that it could be easily adapted to predict contamination in other shellfish of concern.

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Section: Resultssupporting

confidence: 94%

Passive samplers accurately predict PAH levels in resident crayfish

Paulik

Smith

Bergmann

et al. 2016

Science of The Total Environment

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“…Fresh-water crabs are at the top of the food chain in the aquatic environment and are among the commonest animals in rivers and estuaries. In crabs, the hepatopancreas-a large, fatty gland-is the major organ of metabolism and digestion (6). The induction of some forms of CYP by many foreign chemicals has been reported in several crab species.…”

mentioning

confidence: 99%

Alterations of Cytochrome P450-Dependent Monooxygenase Activities in Eriocheir japonicus in Response to Water Pollution

Ishizuka¹,

Hoshi²,

Minamoto³

et al. 1996

Environmental Health Perspectives

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“…In blue crab, PAHs are taken up primarily into the hepatopancreas, where they are metabolized and eliminated (20,39 (41). This may be one mechanism by which organic contaminants can become biologically unavailable for metabolism to reactive intermediates, thereby mitigating toxicity to the adult animal, but promoting toxicity in their offspring.…”

mentioning

confidence: 99%

Long-Term Pyrene Exposure of Grass Shrimp, Palaemonetes pugio, Affects Molting and Reproduction of Exposed Males and Offspring of Exposed Females

Oberdörster¹,

Brouwer²,

Hoexum-Brouwer³

et al. 2000

Environ Health Perspect

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The objective ofthis study was to investigate the impact oflong-term pyrene exposure on molting and reproduction in the model estuarine invertebrate, the grass shrimp (Palaemonetes pugto).Grass shrimp were exosed to measured concentrations of 5.1, 15.0, and 63.4 ppb (pg/L) pyrene for 6 wedss, during which time we determined molting and survivorship. At the end of the exposure, we immediately sacrificed some of the shrimp for biomarker (CYP1A and vitellin) analyses.The remaining shrimp were used to analyze fecundity and embryp survivorship during an additional 6 weeks after termination of pyrene exposure. Male shrimp at the highest pyrene dose (63 ppb) experienced a significant delay in molting and in time until reproduction, and showed elevated ethoxycoumarin o-deethylase (ECOD) activity immediately after the 6-week exposure period. In contrast, 63 ppb pyrene did not affect these parameters in female shrimp. Females produced the same number of eggs per body weight, with high egg viability (98-100%) at all exposure levels, but with decreased survival for the offspring ofthe 63-ppb pyrene-exposed females. In addition, vitellin levels were elevated only in females at 63 ppb pyrene after the 6-week exposure. We hypothesize that the elewted vitellin binds pyrene and keeps it biologically unavailable to adult females, resulting in materal transfer ofpyrene to the embryos. This would account for the lack of effect of pyrene exposure on ECOD activity, molting, and reproduction in the adult females, and for reduced survival of their offspring.

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Fate of petroleum hydrocarbons taken up from food and water by the blue crab Callinectes sapidus

Cited by 114 publications

References 17 publications

Passive samplers accurately predict PAH levels in resident crayfish

Passive samplers accurately predict PAH levels in resident crayfish

Alterations of Cytochrome P450-Dependent Monooxygenase Activities in Eriocheir japonicus in Response to Water Pollution

Long-Term Pyrene Exposure of Grass Shrimp, Palaemonetes pugio, Affects Molting and Reproduction of Exposed Males and Offspring of Exposed Females

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