Fate of U-¹⁴C-Gelatinized and U-¹⁴C-Retrograded Bean Starch in the Rat

Abstract

The fate of retrograded and gelatinized bean starch in the rat was followed using an in vivo radiolabel technique. [U-14 C]Bean starch was obtained by incubating excised unripe pods of broad beans with 14 CO 2 . After extraction and purification, [ 14 C]bean starch was either retrograded or gelatinized and fed to rats by gavage. Rats were housed individually in metabolic cages and were given free access to food and water. After 3 and 18 h of gavage, rats were killed by cervical dislocation and the radioactivity was measured in gut contents, tissues, feces, urine, and CO 2 . The rate and extent of degradation of [ 14 C]starch in the small intestine were lower for 14 C-retrograded starch, as indicated by the high amounts of R-glucans of degree of polymerization >70 in the distal fraction of the small intestine 3 h after gavage. After 18 h of gavage, the percentage of radioactivity present in tissues, feces, and urine was higher in rats fed 14 C-retrograded starch. The [ 14 C]starch degradation products were mainly metabolized by the carcass, pelt, liver and intestinal tissues.

Degradation and metabolism of 14C‐labelled proanthocyanidins from carob (Ceratonia siliqua) pods in the gastrointestinal tract of the rat

“…Animal experiments were performed according to UK Home Office guidelines. Male Wistar Hans rats (∼150 g), previously fed on a stock pelleted diet (CRM(x)), were used for the study 33. Rats (three per group) were dosed by gavage with 0.5 ml of an aqueous suspension of tannins.…”

Methodologies for Increasing the Resistant Starch Content of Food Starches: A Review

“…RS can also be estimated by performing a TS assay on rat fecal material; however, this method does not take into account fermentation, which would occur in the rat bowels (Hoover and Zhou ), thus lowering estimated RS. Additionally, RS may also be estimated by labelling the starch fraction with 13 C (Gerrits and others ) or 14 C (Abia and others ) used for feeding trials, thereby allowing the amount of RS to be quantified by the amount of the labeled carbon leaving the test animal relative to the amount that entered.…”

Metabolic Transformations of U-14C-Resistant and U-14C-Gelatinized Bean Starch in Rat Liver and Body

“…The metabolic transformations of 14 C metabolites produced during degradation of U-14 C-resistant bean starch have been studied in the liver and carcass by comparison with readily digestible U-14 C-gelatinized bean starch. Recent studies on rats fed U-14 C-resistant starch (Abia et al, 1996) have shown that most of the 14 C metabolized by rat tissues was diverted toward carcass (mainly muscle), pelt, and liver. The liver and carcass were therefore chosen for further investigation.…”

“…Preparation of U-14 C-Gelatinized and U-14 C-Resistant Bean Starch. Starch was prepared and purified according to the previous description (Abia et al, 1996). [U-14 C]Starch was obtained from half-filled pods of broad beans incubated under 14 CO2.…”

“…The animals were housed individually in metabolic cages with a broad-spaced grid to minimize coprophagy and were allowed free access to food and water. After 3 and 18 h of feeding, rats were killed by cervical dislocation and immediately dissected (Abia et al, 1996). The carcass (defined as muscle and bone), homogenized in a Waring blender, and the liver were used to study the metabolic fate of [ 14 C]starch degradation products.…”