The present-day road traffic with the persistent change in the type and volume of vehicles needs to be specifically investigated for effective safety management of aging highway infrastructures. Actual traffic data can be implemented in refined procedures for stochastic simulation of road infrastructure performance, structural health monitoring (SHM), definition of weight limits on highways, and traffic-informed structural safety checks. While weigh-in-motion (WIM) systems had been widely used in many countries, their installation on Italian highways was mostly discussed and carried out only after the catastrophic collapse of the Polcevera bridge in 2018. This study presents a statistical data analysis, probabilistic models, and a simulation procedure for highway traffic, based on measurements of two WIM systems located along European route E45 close to Naples, Italy. Different limitations to maximum gross vehicle weight (GVW) were enforced at the locations of the two WIM systems, according to the Italian road code and the Italian guidelines for risk classification, safety assessment and monitoring of existing bridges, respectively. WIM data sets were filtered to exclude erroneous traffic data and vehicle classes defined according to the number of axles and axle distance were statistically characterised, allowing the derivation of probabilistic models for all traffic parameters of interest. A simulation methodology to generate random traffic load from the WIM data is also presented for its possible use in probabilistic performance assessment and traffic informed SHM of road infrastructures such as bridges.