Fatness QTL on chicken chromosome 5 and interaction with sex

Abasht, Behnam; Pitel, Frédérique; Lagarrigue, Sandrine; Bihan-Duval, Élisabeth Le; Roy, Pascale Le; Demeure, Olivier; Vignoles, Florence; Simon, Jean; Cogburn, Larry A.; Aggrey, S. E.; Vignal, Alain; Douaire, Madeleine

doi:10.1186/1297-9686-38-3-297

Cited by 28 publications

(18 citation statements)

References 40 publications

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“…The assignment of the chicken Dicer gene to chromosome 5 at 1040 cRs is consistent with the chicken and human comparative maps and supports the conservation of synteny between the GGA5 and the human chromosomes (Appendix 1). Previous results indicated that a quantitative trait locus (QTL) affecting fatness in male chicken is identified on chromosome 5 (GGA5), and the female fatness QTL is assigned to less than 8 Mb at the distal part of GGA5 (Abasht et al, 2006). Dicer is a possible positional candidate gene.…”

Section: Resultsmentioning

confidence: 99%

Assignment of Dicer Gene to Chicken Chromosome 5 by Radiation Hybrid Panel Mapping

Tian

Liu

et al. 2007

Biochem Genet

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Section: Resultsmentioning

confidence: 99%

Assignment of Dicer Gene to Chicken Chromosome 5 by Radiation Hybrid Panel Mapping

Tian

Liu

et al. 2007

Biochem Genet

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“…Sex specific and fat related QTL have been described on chromosome 5 in chicken [33] and on several chromosomes in mouse [34]. Gene expression studies in male and female F 2 mice have shown a large degree of sexually dimorphic gene expression in several tissues [35,36].…”

Section: Resultsmentioning

confidence: 99%

Genome-wide mapping of Quantitative Trait Loci for fatness, fat cell characteristics and fat metabolism in three porcine F2 crosses

et al. 2010

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BackgroundQTL affecting fat deposition related performance traits have been considered in several studies and mapped on numerous porcine chromosomes. However, activity of specific enzymes, protein content and cell structure in fat tissue probably depend on a smaller number of genes than traits related to fat content in carcass. Thus, in this work traits related to metabolic and cytological features of back fat tissue and fat related performance traits were investigated in a genome-wide QTL analysis. QTL similarities and differences were examined between three F2 crosses, and between male and female animals.MethodsA total of 966 F2 animals originating from crosses between Meishan (M), Pietrain (P) and European wild boar (W) were analysed for traits related to fat performance (11), enzymatic activity (9) and number and volume of fat cells (20). Per cross, 216 (M × P), 169 (W × P) and 195 (W × M) genome-wide distributed marker loci were genotyped. QTL mapping was performed separately for each cross in steps of 1 cM and steps were reduced when the distance between loci was shorter. The additive and dominant components of QTL positions were detected stepwise by using a multiple position model.ResultsA total of 147 genome-wide significant QTL (76 at P < 0.05 and 71 at P < 0.01) were detected for the three crosses. Most of the QTL were identified on SSC1 (between 76-78 and 87-90 cM), SSC7 (predominantly in the MHC region) and SSCX (in the vicinity of the gene CAPN6). Additional genome-wide significant QTL were found on SSC8, 12, 13, 14, 16, and 18. In many cases, the QTL are mainly additive and differ between F2 crosses. Many of the QTL profiles possess multiple peaks especially in regions with a high marker density. Sex specific analyses, performed for example on SSC6, SSC7 and SSCX, show that for some traits the positions differ between male and female animals. For the selected traits, the additive and dominant components that were analysed for QTL positions on different chromosomes, explain in combination up to 23% of the total trait variance.ConclusionsOur results reveal specific and partly new QTL positions across genetically diverse pig crosses. For some of the traits associated with specific enzymes, protein content and cell structure in fat tissue, it is the first time that they are included in a QTL analysis. They provide large-scale information to analyse causative genes and useful data for the pig industry.

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“…Our preliminary analysis of the liver transcriptome in the FL and LL chickens during juvenile development revealed 1,805 differentially expressed (DE) genes [3]. Quantitative trait loci (QTL) analyses of an FL x LL intercross identified a major QTL for abdominal fatness at the distal end of chromosome 5 ( GGA 5) [16,17,33]. Further, the expression quantitative trait loci (eQTL) analysis of GGA 5, involving a three generation intercross of the FL x LL chickens, identified variations in expression of 660 hepatic genes that were correlated with abdominal fatness traits [19].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of abdominal fat in genetically fat and lean chickens reveals adipokines, lipogenic genes and a link between hemostasis and leanness

et al. 2013

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BackgroundThis descriptive study of the abdominal fat transcriptome takes advantage of two experimental lines of meat-type chickens (Gallus domesticus), which were selected over seven generations for a large difference in abdominal (visceral) fatness. At the age of selection (9 wk), the fat line (FL) and lean line (LL) chickens exhibit a 2.5-fold difference in abdominal fat weight, while their feed intake and body weight are similar. These unique avian models were originally created to unravel genetic and endocrine regulation of adiposity and lipogenesis in meat-type chickens. The Del-Mar 14K Chicken Integrated Systems microarray was used for a time-course analysis of gene expression in abdominal fat of FL and LL chickens during juvenile development (1–11 weeks of age).ResultsMicroarray analysis of abdominal fat in FL and LL chickens revealed 131 differentially expressed (DE) genes (FDR≤0.05) as the main effect of genotype, 254 DE genes as an interaction of age and genotype and 3,195 DE genes (FDR≤0.01) as the main effect of age. The most notable discoveries in the abdominal fat transcriptome were higher expression of many genes involved in blood coagulation in the LL and up-regulation of numerous adipogenic and lipogenic genes in FL chickens. Many of these DE genes belong to pathways controlling the synthesis, metabolism and transport of lipids or endocrine signaling pathways activated by adipokines, retinoid and thyroid hormones.ConclusionsThe present study provides a dynamic view of differential gene transcription in abdominal fat of chickens genetically selected for fatness (FL) or leanness (LL). Remarkably, the LL chickens over-express a large number of hemostatic genes that could be involved in proteolytic processing of adipokines and endocrine factors, which contribute to their higher lipolysis and export of stored lipids. Some of these changes are already present at 1 week of age before the divergence in fatness. In contrast, the FL chickens have enhanced expression of numerous lipogenic genes mainly after onset of divergence, presumably directed by multiple transcription factors. This transcriptional analysis shows that abdominal fat of the chicken serves a dual function as both an endocrine organ and an active metabolic tissue, which could play a more significant role in lipogenesis than previously thought.

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Fatness QTL on chicken chromosome 5 and interaction with sex

Cited by 28 publications

References 40 publications

Assignment of Dicer Gene to Chicken Chromosome 5 by Radiation Hybrid Panel Mapping

Assignment of Dicer Gene to Chicken Chromosome 5 by Radiation Hybrid Panel Mapping

Genome-wide mapping of Quantitative Trait Loci for fatness, fat cell characteristics and fat metabolism in three porcine F2 crosses

Transcriptional analysis of abdominal fat in genetically fat and lean chickens reveals adipokines, lipogenic genes and a link between hemostasis and leanness

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