Fatores de risco associados a doenças respiratórias em potros Puro Sangue Inglês do nascimento ao sexto mês de vida

Ribas, Leandro do Monte; Moraes, Carina Martins de; Lins, Luciana Araujo; Flores, Eduardo Furtado; Nogueira, Carlos Eduardo Wayne

doi:10.1590/s0103-84782009005000120

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…The disease has a low mortality rate, but high morbidity, which is highly significant where there are large concentrations of horses. In Brazil, the large number of animals coupled with the need for increased production leads to increased occurrence of this and other diseases and associated economic losses to the Equine (Silva and Vargas, 2006;Ribas et al, 2009). In the state of Rio Grande do Sul strangles outbreaks occur in various areas (Libardoni et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA

Moraes

Conceição

Rocha

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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Strangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, express and characterize the SeM protein of Streptococcus equi subsp. equi, evaluate its use as antigen in indirect ELISA and determine its performance to distinguish sera of negative, vaccinated and positive animals. This was initially performed by cloning the gene encoding the SeM protein and its expression in Escherichia coli. Subsequently, the protein produced was characterized and used as antigen in ELISA. Serum samples for evaluation were taken from 40 negative foals, 46 horses vaccinated with a commercial vaccine against strangles and 46 horses diagnosed with the disease. The test showed high specificity and sensitivity, allowing discrimination between negative and positive, positive and vaccinated animals, and vaccinated animals and negative sera. Thus, it was concluded that the protein produced rSeM, which can be used as antigen for disease diagnosis, and the described ELISA might be helpful to evaluate the immune status of the herd.

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Section: Discussionmentioning

confidence: 99%

Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA

Moraes

Conceição

Rocha

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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“…Respiratory disorders are common in young horses, especially in foals between one to six months of age, and are caused by different infectious agents (Cohen, 1994;Ribas et al, 2009). Foals are more susceptible to infection after six weeks of birth when there is a decrease in passive immunity.…”

Section: Introductionmentioning

confidence: 99%

Virulence, resistance, and genetic relatedness of Escherichia coli and Klebsiella sp. isolated from mule foals

Carneiro

Lessa

Guttmann

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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Respiratory diseases are common in young horses but little is known about such infections in mule foals. This study aimed to characterize Escherichia coli and Klebsiella sp. isolated from tracheal wash (TW) and fecal samples (FS) of mule foals, with or without cytological evidence of respiratory disease. Strains were analyzed against 13 antimicrobials, for presence of Extended spectrum beta-lactamase (ESBL), and virulence genes. Phylogrouping and Randomic (RAPD)-PCR profiles were used to evaluate their genetic relatedness. E. coli strains from TW and FS showed greatest resistance to tetracycline, while Klebsiella strains were mainly resistant to ampicillin; multidrug resistance and ESBL production were also detected. The blaCTX gene prevailed among the E. coli isolates, while the blaSHV gene was more frequently found in K. pneumoniae. The fimH gene was detected in most of the isolates and multiple virulence factors were identified in three E. coli isolates. Most of the E. coli isolates belonged to the B1 phylogroup, but B2 strains displayed more virulence genes. The RAPD assay revealed genetic diversity among strains and was able to distinguish FS isolates from TW isolates. Knowledge of the bacteria associated with the respiratory tract of mule foals is important in the treatment of sick animals.Keywords: Escherichia coli, Klebsiella, respiratory disease, virulence, antimicrobial resistance RESUMO Doenças respiratórias são comuns em potros de equinos, porém pouco se sabe sobre tais infecções em potros de muar. Este estudo buscou caracterizar Escherichia coli e Klebsiella sp. isolados de lavados traqueais (TW) e amostras fecais (FS) de potros de muar com e sem evidências citológicas de doença respiratória. As amostras bacterianas foram testadas contra 13 antimicrobianos, para a presença de genes de resistência estendida às betalactamases (ESBL) e de virulência. Filogrupagem e perfis de PCR randômicos (RAPD) foram usados para avaliar sua relação genética. As amostras de E. coli de TW e FS mostraram maior resistência à tetraciclina, enquanto as amostras de

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Chromobacterium violaceum Infection in a Horse

Hammerschmitt

Rolim

Snel

et al. 2017

Journal of Comparative Pathology

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Fatores de risco associados a doenças respiratórias em potros Puro Sangue Inglês do nascimento ao sexto mês de vida

Cited by 7 publications

References 15 publications

Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA

Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA

Virulence, resistance, and genetic relatedness of Escherichia coli and Klebsiella sp. isolated from mule foals

Chromobacterium violaceum Infection in a Horse

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