Fatty acid biosynthesis by isolated mitochondria from Drosophila melanogaster

Goldin, Herbert H.; Keith, Alec D.

doi:10.1016/0022-1910(68)90001-2

“…While primarily considered a microsomal enzyme, A9 desaturase activity has also been detected in the mitochondrial fraction of D. rnelunoguster [114] and of larval C. cupifutu [1l2]. Separate desaturase mechanisms are suggested by the differential effect of cyanide and carbon monoxide on desaturase activity associated with mitochondrial and microsomal fractions.…”

Section: Desaturation Of Fatty Acidsmentioning

confidence: 99%

“…In addition to direct desaturation, another pathway where the double bond is introduced during chain synthesis has been suggested for Culliphoru erythrocephulu [115,116] and D. rnelunoguster [104,114,117,118]. A similar pathway is proposed for anerobic bacteria; work is needed to confirm this pathway and gain an understanding of its significance in insects.…”

Section: Desaturation Of Fatty Acidsmentioning

confidence: 99%

Fatty acids in insects: Composition, metabolism, and biological significance

Stanley-Samuelson

¹

,

Jurenka

²

,

Cripps

³

et al. 1988

Arch Insect Biochem Physiol

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The composition, metabolism, and significance of fatty acids in insect biology are addressed. Fatty acids enter a number of metabolic pathways not directly related to energy storage and production; the unifying theme is that the fatty acids are not only structurally altered in these pathways, but that the alterations carry them from one area of biological significance into another. This theme is developed by offering a perspective on fatty acids in insects and then reviewing three major areas: 1) fatty acid composition, 2) biosynthesis of fatty acids (including polyunsaturated fatty acids and characteristics of certain biosynthetic enzymes), and 3) the biological significance of fatty acids. This last section includes discussions of the biochemistry of waxes, pheromones, and prostaglandins and the roles of fatty acids as components of defensive secretions. Little is known at the biochemical level about the regulation of fatty acid metabolism, and it is suggested that work in this area represents another frontier in insect biochemistry.

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“…Biosynthesis of haem, a co-factor of cytochrome P-450 enzymes, which modify the long carbon chains in the mitochondria or the smooth endoplasmatic reticulum (ER) (Capdevila, Falck & Estabrook, 1992), involves enzymes acting in the mitochondria. While the role of mitochondria in lipid metabolism is well understood (Goldin & Keith, 1968; Mesmin, 2016; Scharwey, Tatsuta & Langer, 2013; Tyurina et al, 2014; Voelker, 2004), it is not known whether mitochondrial genetic variation is associated with variation of lipid or CHC synthesis, in turn influencing cuticle barrier function.…”

Section: Introductionmentioning

confidence: 99%

The cuticle inward barrier inDrosophila melanogasteris shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet

Dong

¹

,

Dobler

²

,

Dowling

³

et al. 2019

PeerJ

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An important role of the insect cuticle is to prevent wetting (i.e., permeation of water) and also to prevent penetration of potentially harmful substances. This barrier function mainly depends on the hydrophobic cuticle surface composed of lipids including cuticular hydrocarbons (CHCs). We investigated to what extent the cuticle inward barrier function depends on the genotype, comprising mitochondrial and nuclear genes in the fruit fly Drosophila melanogaster, and investigated the contribution of interactions between mitochondrial and nuclear genotypes (mito-nuclear interactions) on this function. In addition, we assessed the effects of nutrition and sex on the cuticle barrier function. Based on a dye penetration assay, we find that cuticle barrier function varies across three fly lines that were captured from geographically separated regions in three continents. Testing different combinations of mito-nuclear genotypes, we show that the inward barrier efficiency is modulated by the nuclear and mitochondrial genomes independently. We also find an interaction between diet and sex. Our findings provide new insights into the regulation of cuticle inward barrier function in nature.

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“…Biosynthesis of haem, a co-factor of cytochrome P-450 enzymes, which modify the long carbon chains in the mitochondria or the smooth endoplasmatic reticulum (ER) (Capdevila et al, 1992), involves enzymes acting in the mitochondria. While the role of mitochondria in lipid metabolism is well understood (Goldin et al, 1968;Mesmin et al, 2016;Scharwey et al, 2013;Tyurinaet al,2014;Voelke,2004), it is not known whether mitochondrial genetic variation is associated with variation of lipid or CHC synthesis, in turn influencing cuticle barrier function.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #3 of "The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet (v0.1)"

2019

0

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An important role of the insect cuticle is to prevent wetting (i.e., permeation of water) and also to prevent penetration of potentially harmful substances. This barrier function mainly depends on the hydrophobic cuticle surface composed of lipids including cuticular hydrocarbons (CHCs). We investigated to what extent the cuticle inward barrier function depends on the genotype, comprising mitochondrial and nuclear genes in the fruit fly Drosophila melanogaster, and investigated the contribution of interactions between mitochondrial and nuclear genotypes (mito-nuclear interactions) on this function. In addition, we assessed the effects of nutrition and sex on the cuticle barrier function. Based on a dye penetration assay, we find that cuticle barrier function varies across three fly lines that were captured from geographically separated regions in three continents. Testing different combinations of mito-nuclear genotypes, we show that the inward barrier efficiency is modulated by the nuclear and mitochondrial genomes independently. We also find an interaction between diet and sex. Our findings provide new insights into the regulation of cuticle inward barrier function in nature.

show abstract

Fatty acid biosynthesis by isolated mitochondria from Drosophila melanogaster

Cited by 22 publications

References 21 publications

Fatty acids in insects: Composition, metabolism, and biological significance

Fatty acids in insects: Composition, metabolism, and biological significance

The cuticle inward barrier inDrosophila melanogasteris shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet

Peer Review #3 of "The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet (v0.1)"

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