Fatty Acid Composition in Ergosteryl Esters and Triglycerides from the Fungus <i>Ganoderma lucidum</i>

Deng, Zilong; Yuan, Jianping; Zhang, Yan; Xu, Xiao‐Ming; Wu, Chou-Fei; Peng, Juan; Wang, Jiang-Hai

doi:10.1007/s11746-013-2296-y

Cited by 8 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enriching the existing literature with new data on compounds produced by different isolates and on the strategy to extract them, especially from spores, may be valuable for the use of G. lucidum in the biomedical field. Although a few investigators have already studied the fatty acids in the triglycerides from G. lucidum [16], in general referred to the fruiting body [17], there are few information on the extraction processes.…”

Section: Introductionmentioning

confidence: 99%

Fatty Acids from Ganoderma lucidum Spores: Extraction, Identification and Quantification

et al. 2020

View full text Add to dashboard Cite

Ganoderma lucidum is a rich and promising source of bioactive compounds capable of positively influencing the human health. For this reason, G. lucidum is the target of vigorous investigational activities, focusing on the extraction of substances with potential biomedical applications, as well as on the improvement of analytical methodologies for their screening. In the present study, three different extraction processes (i.e., extraction with n-hexane, in Soxhlet apparatus with n-hexane and reflux with chloroform) were employed to evaluate the lipid content of G. lucidum spores. The experimental data from the produced extracts were compared with ones from the commercial liquid extract obtained with supercritical CO2 extraction of G. lucidum spores. To achieve this goal, the gas chromatography-mass spectrometry (GC-MS) methodology was optimized for an accurate and reliable identification and quantification of fatty acids in triglycerides of G. lucidum spores. Significant amounts of oleic and palmitic acids were found in the examined extracts. In particular, our findings show that economical and convenient extraction procedures, which are simple, low cost and no need for expensive tools, allow to produce extracts with a lipid composition similar to the one obtained with hard strategies of extraction.

show abstract

Section: Introductionmentioning

confidence: 99%

Fatty Acids from Ganoderma lucidum Spores: Extraction, Identification and Quantification

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Hence, this difference of stable carbon isotopes may reach a detectable level with the extension of time. Based on the above principle of isotope fractionation, we may study the metabolism and development of organisms as well as the authentication of biological products 6 , 24 – 26 , 34 – 36 .…”

Section: Discussionmentioning

confidence: 99%

Fungus-larva relation in the formation of Cordyceps sinensis as revealed by stable carbon isotope analysis

Guo¹,

Hong²,

Zhou³

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

For more than one thousand years, Cordyceps sinensis has been revered as a unique halidom in the Qinghai-Tibetan Plateau for its mysterious life history and predominant medicinal values. This mysterious fungus-larva symbiote also attracted the over-exploitation, while several problems on the initial colonization of Ophiocordyceps sinensis in the host larva have constrained artificial cultivation. In this work, stable carbon isotope analysis was employed to analyse the subsamples of C. sinensis from 5 representative habitats. The results demonstrated that these samples possessed similar δ13C profiles, i.e., a steady ascending trend from the top to the bottom of stroma, occurrence of the δ13C maximum at the head, a slight decrease from the head to the end of thorax, a sharply descent trend from the end of thorax to the forepart of abdomen, and maintenance of lower δ13C values in the rest parts of abdomen. Based on the data, we consider that the site near the head of the host larva may be the initial target attacked by O. sinensis, and the fungus growth is closely related to the digestive tract of its host larva. The growth stages of O. sinensis are accordingly speculated as the symptom-free, symptom-appearing, and stroma-germinating stages.

show abstract

“…As a component of cell membranes, sterols may affect various functions of cell membranes, such as maintaining the permeability and fluidity of cell membranes [ 15 ]. Ergosterols are found almost solely in fungi, enriched in the plasma membrane, and could be used as an indicator of fungal biomass.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Proteomic Profiling of Fungal Growth, Development, and Ochratoxin A Production in Aspergillus ochraceus on High- and Low-NaCl Cultures

Wang

Guan

Lin

et al. 2021

Toxins

View full text Add to dashboard Cite

Dry-cured meat products are worldwide food with high-salt content, and filamentous fungi are beneficial to the maturation process. However, some salt-tolerant strains of Aspergillus and Penicillium produce ochratoxin A (OTA) on these products and thus threaten food safety. In our study, proteomic analysis was performed to reveal the mechanism of adaptability to high-salt environment by Aspergillus ochraceus. Twenty g/L and 70 g/L NaCl substrates were used to provide medium- and high-NaCl content environments, respectively. The NaCl addition could induce fungal growth, but only 20 g/L NaCl addition could induce spore production while 70 g/L repressed it. Proteomics analysis identified 2646 proteins in A. ochraceus fc-1, of which 237 and 251 were differentially expressed with 20 g/L and 70 g/L NaCl addition, respectively. Potential factors affecting fungal growth and development were identified by GO and KEGG analyses of biological process, cellular component, and molecular function terms. The results revealed that ergosterol synthesis pathway was significantly upregulated with 20 g/L and 70 g/L NaCl addition. However, fungal growth and development including OTA production were complex processes associated with many factors including nutrient uptake, cell membrane integrity, cell cycle, energy metabolism, intracellular redox homeostasis, protein synthesis and processing, autophagy, and secondary metabolism. Reactive oxygen species may be an important window to understand the mechanism that medium-salt content was conducive to intracellular signal transduction while high-salt content caused oxidative stress. The findings would help to improve the processes and storage conditions of dry-cured meat products.

show abstract

Fatty Acid Composition in Ergosteryl Esters and Triglycerides from the Fungus Ganoderma lucidum

Cited by 8 publications

References 26 publications

Fatty Acids from Ganoderma lucidum Spores: Extraction, Identification and Quantification

Fatty Acids from Ganoderma lucidum Spores: Extraction, Identification and Quantification

Fungus-larva relation in the formation of Cordyceps sinensis as revealed by stable carbon isotope analysis

Quantitative Proteomic Profiling of Fungal Growth, Development, and Ochratoxin A Production in Aspergillus ochraceus on High- and Low-NaCl Cultures

Contact Info

Product

Resources

About