The present experiments were carried out during two successive seasons of 2007-2008 and 2008-2009 at the Mushroom Research Lab., Faculty of Agriculture, Cairo University. A mushroom strain of Flammulina velutipes (Curtis) Singer used in this study was provided by Fujian Agricultural Universiy, China. This study aimed to achieve the following goals:1-Using some agricultural wastes; i. e., rice straw, wheat straw, Faba bean stalks and maize cobs as well as soft sawdust for the cultivation of a strain of Flammulina velutipes. 2-Determinate the effect of these wastes on growth parameters and yield. 3-Evaluate the produced mushroom fruit bodies for their nutritional value. Maize cob wastes caused the highest values of total yield first and second flush weight as well as biological efficiency and number of days from spawning till first flush. Meanwhile, rice straw resulted in the lowest first flush percentage weight of clusters and branches. Weight of clusters was the highest when grown in maize cobs. Rice straw caused the lowest values of number and weight of clusters and branches. Flammulina velutipes grown on Faba bean stalks recorded the highest values of crude protein and fibers. Meanwhile, maize cobs resulted in the highest values of total carbohydrates.It was observed that there were changes in the chemical components of the substrates after cultivation as compared to those before cultivation. Crude protein and ash increased after cultivation. Meanwhile, fibers, fats, and total carbohydrates decreased after the substrate cultivation.