2000
DOI: 10.1093/ps/79.8.1168
|View full text |Cite
|
Sign up to set email alerts
|

Fatty Acid Determination in Chicken Egg Yolk: A Comparison of Different Methods

Abstract: Four different methods (direct-methylation, saponification, chloroform-methanol extraction, and postextraction saponification) were compared to determine the fatty acids in egg yolk. About 50 mg of pooled egg yolk samples, with C23:0 as an internal standard, was used for all assays. No difference (P > 0.05) was observed among the four methods for C17:0, C18:0, C18:1, C20:1, C18:2n-6, and C22:6n-3 content of egg yolk. Direct saponification resulted in a lower (P < 0.05) content of C14:0, C16:1, C18:3n-3, and C2… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

2
43
0
6

Year Published

2003
2003
2017
2017

Publication Types

Select...
10

Relationship

1
9

Authors

Journals

citations
Cited by 83 publications
(55 citation statements)
references
References 10 publications
2
43
0
6
Order By: Relevance
“…These analyses were performed following the chloroformmethanol extraction method described by Wang et al (2000). A fused silica capillary column (Omegawax 320, 30 m × 0.32 mm ID, 0.25 μm) on a Shimadzu GC-14B chromatograph, equipped with an autosampler and fl ame ionization detector, was used to separate and quantify the fatty acid methyl esters.…”
Section: Fatty Acid Compositionmentioning
confidence: 99%
“…These analyses were performed following the chloroformmethanol extraction method described by Wang et al (2000). A fused silica capillary column (Omegawax 320, 30 m × 0.32 mm ID, 0.25 μm) on a Shimadzu GC-14B chromatograph, equipped with an autosampler and fl ame ionization detector, was used to separate and quantify the fatty acid methyl esters.…”
Section: Fatty Acid Compositionmentioning
confidence: 99%
“…The ether extract from ghee residue was utilized for the analysis of fatty acid composition using Gas Chromatography. The standardization of fatty acid profile was done with egg yolk lipid as per the procedure described by Wang, et al (2000). The fatty acids analyzed include Myristic acid, Palmitic acid, Palmitoleic acid, Stearic acid, Oleic acid, Linoleic acid, Linolenic acid, Arachidic acid, Behenic acid, Ecosapentaenoic acid and Docosahexaenoic acid, the values of which were expressed as per cent dry matter basis.…”
Section: Mineral Profile Of Ghee Residuementioning
confidence: 99%
“…Fatty acid methyl esters were identified by comparison with retention times of standard mixture. The fatty acid content of egg yolk was calculated using the following formula equation 2 [17,18]:…”
Section: Fatty Acids Determinationmentioning
confidence: 99%