Fatty Acid Pattern, Oxidation Product Development, and Antioxidant Loss in Muscle Tissue of Rainbow Trout and Dicentrarchus labrax during Growth

Abstract

The levels of hydrophilic, lipophilic, and enzymatic antioxidants, the oxidative damage to lipids and proteins, and the fatty acid patterns of triglyceride and phospholipid fractions were assayed in fresh muscle tissue of rainbow trouts (Oncorhynchus mykiss) and sea basses (Dicentrarchus labrax) during aging, to investigate the correlation between oxidative stress and aging processes in fish. The present studies suggests that lipid peroxidation and accumulation of oxidized proteins during in vivo aging are most likely to be linked with an age-dependent decline of lipophilic antioxidants (CoQH(2), CoQ, and vitamin E) and vitamin C contents in muscle tissue, whereas fish aging is not linked to a decline in antioxidant enzymes and reduced glutathione levels. Lipophilic antioxidant and vitamin C levels represent a reliable marker of oxidative stress during aging, and their determination might be useful for the assessment of fish age.

Oxidative status and histological changes in sea bass larvae muscle in response to high dietary content of docosahexaenoic acid DHA

“…Thiobarbituric acid reactive substances were significantly influenced by dietary DHA levels, being significantly increased in sea bass larvae fed the highest dietary DHA content, irrespective of vitamin E content suggesting its ineffective protective role against oxidation. Moreover, TBARS values obtained in this work were quite elevated compared with other studies in sea bass adults and juveniles exposed to different pollutants (Passi et al 2004;Almeida et al 2010). The fact that TBARS values were higher in this study could be attributed to the poor activity of the antioxidant system in fish larvae.…”

“…Moreover, TBARS values obtained in this work were quite elevated compared with other studies in sea bass adults and juveniles exposed to different pollutants (Passi et al . ; Almeida et al . ).…”

Age-dependent patterns of antioxidants in Arctica islandica from six regionally separate populations with different lifespans

“…This upward trend of antioxidants with age is rarely observed in ectotherms. SOD and glutathione peroxidase increased with age in the shrimp Aristeus antennatus (Mourente & Diaz-Salvago 1999), and glutathione concentration was higher in older than in younger Oncorhynchus mykiss (Passi et al 2004). This trend may indicate the increased demand for antioxidants at advanced age but could also be an effect of sampling the 'late survivors'.…”

Effect of dietary supplementation of probiotics and palm fruits extracts on the antioxidant enzyme gene expression in the mucosae of gilthead seabream (Sparus aurata L.)

“…Oxygen continuously generates ROS in many tissues as products of oxidative metabolism [57]. Furthermore, there are many natural sources of oxidative stress, for example, exposure to environmental oxidants, toxins like heavy metals, ionising and UV irradiation, heat shock and inflammation.…”