Fatty Acid Profile Changes During Gradual Soil Water Depletion in Oats Suggests a Role for Jasmonates in Coping With Drought

Abstract

Although often investigated within the context of plant growth and development and/or seed composition, plant lipids have roles in responses to environment. To dissect changes in lipid and fatty acid composition linked to drought tolerance responses in oats, we performed a detailed profiling of (>90) different lipids classes during a time course of water stress. We used two oat cultivars, Flega and Patones previously characterized as susceptible and tolerant to drought, respectively. Significant differences in lipid classes (mono, di and triacylglycerols; [respectively MAG, DAG, and TAG] and free fatty acids [FFA]) and in their fatty acid (FA) composition was observed between cultivars upon drought stress. In Flega there was an increase of saturated FAs, in particular 16:0 in the DAG and TAG fractions. This led to significant lower values of the double bond index and polyunsaturated/saturated ratio in Flega compared with Patones. By contrast, Patones was characterized by the early induction of signaling-related lipids and fatty acids, such as DAGs and linolenic acid. Since the latter is a precursor of jasmonates, we investigated further changes of this signaling molecule. Targeted measurements of jasmonic acid (JA) and Ile-JA indicated early increases in the concentrations of these molecules in Patones upon drought stress whereas no changes were observed in Flega. Altogether, these data suggest a role for jasmonates and specific fatty acids in different lipid classes in coping with drought stress in oat.

Genome-wide association study identifies candidate genes related to oleic acid content in soybean seeds

“…Overexpression of BAG7 can increase plant sensitivity to temperature, and BAG4 encodes anti-apoptotic genes that have been reported to confer tolerance to salinity and drought stresses in transgenic tobacco [ 24 ]. Drought treatment at different growth stages also contributed to differences in fatty acids [ 25 ]. The fatty acid composition and amino acid composition were significantly affected by drought stress [ 26 ].…”

Deciphering Root Architectural Traits Involved to Cope With Water Deficit in Oat

“…Recent studies have tackled the drought tolerance response of oat. These covered different aspects focusing on adaptation and yield potential (Sánchez-Martín et al, 2014; Sadras et al, 2017; Sánchez-Martín et al, 2018), on the role of different metabolites during drought tolerance responses (Sánchez-Martín et al, 2015; Sánchez-Martín et al, 2017; Gao et al, 2018; Canales et al, 2019), or on the identification of physiological and biochemical markers to select drought tolerant genotypes (Rabiei et al, 2012; Sánchez-Martín et al, 2012; Marcinska et al, 2017; Rispail et al, 2018). However, and following the same trend as for other crops, very few information are available regarding root responses engaged for drought tolerance in oat.…”

Salicylic acid regulates polyamine biosynthesis during drought responses in oat

“…To test this hypothesis, oat plants from the resistant Patones and susceptible Flega cultivars were grown and subjected to drought as previously. 5,11,12 At the beginning of the drought treatment, the plants were sprayed with 100 μM SA or 4-hydroxibenzoic acid (4-hBA), an isomer of SA with no biological activity 13 as control. As previously, 5 we observed an alleviation of drought symptoms in SA-treated plants.…”